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Photobleaching and Sensitized Emission-Based Methods for the Detection of Förster Resonance Energy Transfer

Zimmermann, Timo·2019·Methods in Molecular Biology
DOI 10.1007/978-1-4939-9686-5_12
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Abstract

Förster resonance energy transfer (FRET) is a non-radiative interaction between two molecules that happens at distances in the range of a few nanometers. Using FRET interactions between suitably selected fluorophores allows to study molecular interactions or conformational changes of single molecules on fluorescence microscopes even though the optical resolution of the microscope is limited to distances that are almost two orders of magnitude higher.In this chapter several variants of FRET detection methods are described that are based either on the targeted photobleaching of one of the participating molecule species or on the direct detection of the fluorescence signal that is created as a result of the FRET interactions.

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Crossref · 10.1007/978-1-4939-9686-5_12

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  • For FRET analysis, donor-only (Cy3) and acceptor-only (Cy5) samples were imaged in each imaging session to calculate the donor-emission crosstalk with the acceptor emission (beta parameter) and the direct excitation of the acceptor by the donor excitation laser line (gamma parameter).

    “For FRET analysis, donor-only (Cy3) and acceptor-only (Cy5) samples were imaged in each imaging session to calculate the donor-emission crosstalk with the acceptor emission (beta parameter) and the direct excitation of the acceptor by the donor excitation laser line (gamma parameter) [75, 80].”

    From Transmembrane protein 97 is a potential synaptic amyloid beta receptor in human Alzheimer’s disease (2024) · cites this paper as [80]

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Metadata

Authors
Zimmermann, T.
Venue
Methods in Molecular Biology
Publisher
Springer New York
Published
2019
Pages
235-274
ISSN
  • 1064-3745
  • 1940-6029
DOI
10.1007/978-1-4939-9686-5_12
Cited by
5
References
0
Language
en
Licence
www.springer.com/tdm (tdm)

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