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Cited by 279

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Understanding Factors that Limit Enzymatic Hydrolysis of Biomass

Laureano-Perez, Lizbeth, Teymouri, Farzaneh, Alizadeh, Hasan, Dale, Bruce E.·2009·Twenty-Sixth Symposium on Biotechnology for Fuels and Chemicals

Abstract

Spectroscopic characterization of both untreated and treated material is being performed in order to determine changes in the biomass and the effects of pretreatment on crystallinity, lignin content, selected chemical bonds, and depolymerization of hemicellulose and lignin. The methods used are X-ray diffraction for determination of cellulose crystallinity (CrI); diffusive reflectance infrared (DRIFT) for changes in C-C and C-O bonds; and fluorescence to determine lignin content. Changes in spectral characteristics and crystallinity are statistically correlated with enzymatic hydrolysis results to identify and better understand the fundamental features of biomass that govern its enzymatic conversion to monomeric sugars. Models of the hydrolysis initial rate and 72 h extent of conversion were developed and evaluated. Results show that the hydrolysis initial rate is most influenced by the cellulose crystallinity, while lignin content most influences the extent of hydrolysis at 72 h. However, it should be noted that in this study only crystallinity, lignin, and selected chemical bonds were used as inputs to the models. The incorporation of additional parameters that affect the hydrolysis, like pore volume and size and surface area accessibility, would improve the predictive capability of the models.

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Metadata

Authors
Laureano-Perez, L., Teymouri, F., Alizadeh, H., & Dale, B. E.
Venue
Twenty-Sixth Symposium on Biotechnology for Fuels and Chemicals
Publisher
Humana Press
Published
2009-03-06
Pages
1081-1099
OpenAlex
W1552219067
Cited by
279
References
21
FWCI
43.12
Type
conference paper
Language
en
Open access
closed

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CoChat. (2026). Citation check: Understanding Factors that Limit Enzymatic Hydrolysis of Biomass. https://app.cochat.ai/papers/10.1007/978-1-59259-991-2_91
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Laureano-Perez, L., Teymouri, F., Alizadeh, H., & Dale, B. E. (2009). Understanding Factors that Limit Enzymatic Hydrolysis of Biomass. Twenty-Sixth Symposium on Biotechnology for Fuels and Chemicals. https://doi.org/10.1007/978-1-59259-991-2_91

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