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Transgenesis in Zebrafish with the Tol2 Transposon System
Abstract
The zebrafish (Danio rerio) is a useful model for genetic studies of vertebrate development. Its embryos are transparent and develop rapidly outside the mother, making it feasible to visualize and manipulate specific cell types in the living animal. Zebrafish is well suited for transgenic manipulation since it is relatively easy to collect large numbers of embryos from adult fish. Several approaches have been developed for introducing transgenes into the zebrafish germline, from the injection of naked DNA to transposon-mediated integration. In particular, the Tol2 transposable element has been shown to create insertions in the zebrafish genome very efficiently. By using Tol2, gene trap and enhancer trap vectors containing the GFP reporter gene or yeast transcription activator Gal4 gene have been developed. Here we outline methodology for creating transgenic zebrafish using Tol2 vectors, and their applications to visualization and manipulation of specific tissues or cells in vivo and for functional studies of vertebrate neural circuits.
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Bhatia, S., Bengani, H., Fish, M., Brown, A., Divizia, M. T., de Marco, R., Damante, G., Grainger, R., van Heyningen, V., & Kleinjan, D. A. (2013) · The American Journal of Human Genetics · doi:10.1016/j.ajhg.2013.10.028 · Cited by 196
Patterson, L. B., & Parichy, D. M. (2013) · PLoS Genetics · doi:10.1371/journal.pgen.1003561 · Cited by 170
In situ differentiation of iridophore crystallotypes underlies zebrafish stripe patterning
Gur, D., Bain, E. J., Johnson, K. R., Aman, A. J., Pasolli, H. A., Flynn, J. D., Allen, M. C., Deheyn, D. D., Lee, J. C., Lippincott-Schwartz, J., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-20088-1 · Cited by 69
Evolution of Endothelin signaling and diversification of adult pigment pattern in Danio fishes
Spiewak, J. E., Bain, E. J., Liu, J., Kou, K., Sturiale, S. L., Patterson, L. B., Diba, P., Eisen, J. S., Braasch, I., Ganz, J., et al. (2018) · PLOS Genetics · doi:10.1371/journal.pgen.1007538 · Cited by 58
Ephrin‐ A 1‐ EphA4 signaling negatively regulates myelination in the central nervous system
Harboe, M., Torvund‐Jensen, J., Kjaer‐Sorensen, K., & Laursen, L. S. (2018) · Glia · doi:10.1002/glia.23293 · Cited by 56
Longitudinal in vivo imaging of adult Danionella cerebrum using standard confocal microscopy
Lam, P.-Y. (2022) · Disease Models & Mechanisms · doi:10.1242/dmm.049753 · Cited by 33
Eom, D. S., Patterson, L. B., Bostic, R. R., & Parichy, D. M. (2021) · Developmental Biology · doi:10.1016/j.ydbio.2021.04.007 · Cited by 23
Huang, D., Kapadia, E. H., Liang, Y., Shriver, L. P., Dai, S., Patti, G. J., Humbel, B. M., Laudet, V., & Parichy, D. M. (2025) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2424180122 · Cited by 15
Metadata
- Authors
- Suster, M. L., Kikuta, H., Urasaki, A., Asakawa, K., & Kawakami, K.
- Venue
- Methods in Molecular Biology
- Publisher
- Humana Press
- Published
- 2009
- Volume
- 561
- Pages
- 41-63
- ISSN
- 1064-3745 (ISSN-L)
- 1940-6029
- OpenAlex
- W164317671
- Cited by
- 232
- References
- 0
- FWCI
- 16.79
- Type
- book chapter
- Language
- en
- Open access
- closed
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