The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Identification of ROS Using Oxidized DCFDA and Flow-Cytometry
Abstract
Cells constantly generate reactive oxygen species (ROS) during aerobic metabolism. The ROS generation plays an important protective and functional role in the immune system. The cell is armed with a powerful antioxidant defense system to combat excessive production of ROS. Oxidative stress occurs in cells when the generation of ROS overwhelms the cells' natural antioxidant defenses. ROS and the oxidative damage are thought to play an important role in many human diseases including cancer, atherosclerosis, other neurodegenerative diseases and diabetes. Thus, establishing their precise role requires the ability to measure ROS accurately and the oxidative damage that they cause. There are many methods for measuring free radical production in cells. The most straightforward techniques use cell permeable fluorescent and chemiluminescent probes. 2'-7'-Dichlorodihydrofluorescein diacetate (DCFH-DA) is one of the most widely used techniques for directly measuring the redox state of a cell. It has several advantages over other techniques developed. It is very easy to use, extremely sensitive to changes in the redox state of a cell, inexpensive and can be used to follow changes in ROS over time.
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Cites
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Quantitative flux analysis reveals folate-dependent NADPH production
Fan, J., Ye, J., Kamphorst, J. J., Shlomi, T., Thompson, C. B., & Rabinowitz, J. D. (2014) · Nature · doi:10.1038/nature13236 · Cited by 1,114
SIRT3 Opposes Reprogramming of Cancer Cell Metabolism through HIF1α Destabilization
Finley, L. W. S., Carracedo, A., Lee, J., Souza, A., Egia, A., Zhang, J., Teruya-Feldstein, J., Moreira, P. I., Cardoso, S. M., Clish, C. B., et al. (2011) · Cancer Cell · doi:10.1016/j.ccr.2011.02.014 · Cited by 782
Extramedullary Hematopoiesis Generates Ly-6C high Monocytes That Infiltrate Atherosclerotic Lesions
Robbins, C. S., Chudnovskiy, A., Rauch, P. J., Figueiredo, J.-L., Iwamoto, Y., Gorbatov, R., Etzrodt, M., Weber, G. F., Ueno, T., van Rooijen, N., et al. (2012) · Circulation · doi:10.1161/circulationaha.111.061986 · Cited by 482
Serine Catabolism Regulates Mitochondrial Redox Control during Hypoxia
Ye, J., Fan, J., Venneti, S., Wan, Y.-W., Pawel, B. R., Zhang, J., Finley, L. W. S., Lu, C., Lindsten, T., Cross, J. R., et al. (2014) · Cancer Discovery · doi:10.1158/2159-8290.cd-14-0250 · Cited by 452
“ROS Measurement and Cell Death Assay Cellular ROS was measured according to published protocols (47).”
MethodAlizadeh, D., Trad, M., Hanke, N. T., Larmonier, C. B., Janikashvili, N., Bonnotte, B., Katsanis, E., & Larmonier, N. (2014) · Cancer Research · doi:10.1158/0008-5472.can-13-1545 · Cited by 416
Metadata
- Authors
- Eruslanov, E., & Kusmartsev, S.
- Venue
- Methods in Molecular Biology
- Publisher
- Humana Press
- Published
- 2009-09-30
- Volume
- 594
- Pages
- 57-72
- ISSN
- 1064-3745 (ISSN-L)
- 1940-6029
- OpenAlex
- W2398664613
- Cited by
- 1,321
- References
- 8
- FWCI
- 24.41
- Type
- book chapter
- Language
- en
- Open access
- closed
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- Eruslanov, E., & Kusmartsev, S. (2010). Identification of ROS Using Oxidized DCFDA and Flow-Cytometry. Methods in Molecular Biology. https://doi.org/10.1007/978-1-60761-411-1_4
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