The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
Detection of Necrosis by Release of Lactate Dehydrogenase Activity
Open accessAbstract
Apoptosis and necrosis are two major forms of cell death observed in normal and disease pathologies. Although there are many assays for detection of apoptosis, relatively few assays are available for measuring necrosis. A key signature for necrotic cells is the permeabilization of the plasma membrane. This event can be quantified in tissue culture settings by measuring the release of the intracellular enzyme lactate dehydrogenase (LDH). When combined with other methods, measuring LDH release is a useful method for the detection of necrosis. In this chapter, we describe the step-by-step procedure for detection of LDH release from necrotic cells using a microtiter plate-based colorimetric absorbance assay.
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Cited by
Toxicity of Nanomaterials: Exposure, Pathways, Assessment, and Recent Advances
Ganguly, P., Breen, A., & Pillai, S. C. (2018) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.8b00068 · Cited by 341
Lactate dehydrogenase activity drives hair follicle stem cell activation
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Metadata
- Authors
- Chan, F. K.-M., Moriwaki, K., & De Rosa, M. J.
- Venue
- Methods in Molecular Biology
- Publisher
- Humana Press
- Published
- 2013
- Volume
- 979
- Pages
- 65-70
- ISSN
- 1064-3745 (ISSN-L)
- 1940-6029
- OpenAlex
- W1598408991
- Cited by
- 786
- References
- 11
- FWCI
- 44.51
- Type
- book chapter
- Language
- en
- Open access
- green
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