Reproductive Sciences in Animal Conservation

Resurrecting biodiversity: Advanced assisted reproductive technologies and biobanking

Bolton, R. L., Mooney, A., Pettit, M. T., Bolton, A. E., Morgan, L., Drake, G. J., Appeltant, R., Walker, S. L., Gillis, J. D., & Hvilsom, C. (2022) · Reproduction and Fertility · doi:10.1530/raf-22-0005 · Cited by 108 · see citing works

Zoo animal welfare assessment: where do we stand?

Tallo-Parra, O., Salas, M., & Manteca, X. (2023) · Animals · doi:10.3390/ani13121966 · Cited by 45 · see citing works

Healthy Cattle Microbiome and Dysbiosis in Diseased Phenotypes

Khalil, A., Batool, A., & Arif, S. (2022) · Ruminants · doi:10.3390/ruminants2010009 · Cited by 44 · see citing works

Modified, optimized method of determination of Tributyltin (TBT) contamination in coastal water, sediment and biota in Sri Lanka

Bandara, K. R. V., Chinthaka, S. D. M., Yasawardene, S. G., & Manage, P. M. (2021) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2021.112202 · Cited by 35 · see citing works

The Increasing Role of Short-Term Sperm Storage and Cryopreservation in Conserving Threatened Amphibian Species

Anastas, Z. M., Byrne, P. G., O’Brien, J. K., Hobbs, R. J., Upton, R. M. O., & Silla, A. J. (2023) · Animals · doi:10.3390/ani13132094 · Cited by 30 · see citing works

Understanding and Assisting Reproduction in Wildlife Species Using Microfluidics

Gac, S. L., Ferraz, M. D. A. M. M., Venzac, B., & Comizzoli, P. (2020) · Trends in biotechnology · doi:10.1016/j.tibtech.2020.08.012 · Cited by 25 · see citing works

The Chemical Ecology of Elephants: 21st Century Additions to Our Understanding and Future Outlooks

Schulte, B. A., & LaDue, C. A. (2021) · Animals · doi:10.3390/ani11102860 · Cited by 24 · see citing works

Ethical Analysis of the Application of Assisted Reproduction Technologies in Biodiversity Conservation and the Case of White Rhinoceros (Ceratotherium simum) Ovum Pick-Up Procedures

Biasetti, P., Hildebrandt, T. B., Göritz, F., Hermes, R. E., Holtze, S., Galli, C., Lazzari, G., Colleoni, S., Pollastri, I., Spiriti, M. M., et al. (2022) · Frontiers in Veterinary Science · doi:10.3389/fvets.2022.831675 · Cited by 20 · see citing works

The effect of Chlamydia infection on koala (Phascolarctos cinereus) semen quality

Hulse, L., Beagley, K. W., Larkin, R., Nicolson, V. N., Gosálvez, J., & Johnston, S. D. (2021) · Theriogenology · doi:10.1016/j.theriogenology.2021.03.016 · Cited by 20 · see citing works

Self-adaptive and efficient propulsion of Ray sperms at different viscosities enabled by heterogeneous dual helixes

Wang, P., Azad, M., Yang, X., Martelli, P. R., Cheung, K. Y., Shi, J., & Shen, Y. (2021) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2024329118 · Cited by 19 · see citing works

Allostatic Load Indices With Cholesterol and Triglycerides Predict Disease and Mortality Risk in Zoo-Housed Western Lowland Gorillas ( Gorilla gorilla gorilla )

Edes, A. N., Edwards, K. L., Wolfe, B. A., Brown, J. L., & Crews, D. E. (2020) · Biomarker Insights · doi:10.1177/1177271920914585 · Cited by 20 · see citing works

Opportunities and Limitations for Reproductive Science in Species Conservation

Holt, W. V., & Comizzoli, P. (2021) · Annual Review of Animal Biosciences · doi:10.1146/annurev-animal-013120-030858 · Cited by 19 · see citing works

First Evaluations and Cryopreservation of Semen Samples from Sunda Clouded Leopards (Neofelis diardi)

Zainuddin, Z. Z., Tarmizi, M. R. M., Yap, K. C., Comizzoli, P., & Sipangkui, S. (2020) · Animals · doi:10.3390/ani10061072 · Cited by 17 · see citing works

Natural marine products as antiprotozoal agents against amitochondrial parasites

Estrella-Parra, E. A., Arreola, R., Álvarez-Sánchez, M. E., Torres‐Romero, J. C., Rojas-Espinosa, O., la Cruz-Santiago, J. A. D., Martinez-Benitez, M. B., López‐Camarillo, C., Lara‐Riegos, J., Arana‐Argáez, V. E., et al. (2022) · International Journal for Parasitology Drugs and Drug Resistance · doi:10.1016/j.ijpddr.2022.05.003 · Cited by 15 · see citing works

Modelling Genetic Benefits and Financial Costs of Integrating Biobanking into the Captive Management of Koalas

Howell, L. G., Johnston, S. D., O’Brien, J. K., Frankham, R., Rodger, J. C., Ryan, S. A., Beranek, C. T., Clulow, J., Hudson, D. S., & Witt, R. R. (2022) · Animals · doi:10.3390/ani12080990 · Cited by 15 · see citing works

Paradigm shift in frog sperm cryopreservation: reduced role for non-penetrating cryoprotectants

Upton, R. M. O., Clulow, S., Colyvas, K., Mahony, M. J., & Clulow, J. (2023) · Reproduction · doi:10.1530/rep-22-0486 · Cited by 15 · see citing works

Cryopreservation Cooling Rate Impacts Post-Thaw Sperm Motility and Survival in Litoria booroolongensis

Hobbs, R. J., Upton, R. M. O., Calatayud, N. E., Silla, A. J., Daly, J., McFadden, M., & O’Brien, J. K. (2023) · Animals · doi:10.3390/ani13193014 · Cited by 13 · see citing works

Marsupials the alternative therians – From gametes to birth

Rodger, J. C. (2020) · Theriogenology · doi:10.1016/j.theriogenology.2020.02.027 · Cited by 12 · see citing works

Urine metabolomics reveals the effects of confined environment on mating choice in adult male giant pandas

Zhang, M., Wang, X., Zhang, X.-H., & Hou, R. (2022) · Physiology & Behavior · doi:10.1016/j.physbeh.2022.113744 · Cited by 12 · see citing works

Investigation of pathology associated with Chlamydia pecorum infection in the male reproductive tract, and the effect on spermatogenesis and semen quality in the koala (Phascolarctos cinereus)

Hulse, L., Palmieri, C., Beagley, K. W., Larkin, R., Keeley, T., Gosálvez, J., & Johnston, S. D. (2021) · Theriogenology · doi:10.1016/j.theriogenology.2021.12.011 · Cited by 11 · see citing works

Biobanks, offspring fitness and the influence of developmental plasticity in conservation biology

Holt, W. V. (2023) · Animal Reproduction · doi:10.1590/1984-3143-ar2023-0026 · Cited by 10 · see citing works

Optimal cooling rates for sperm cryopreservation in a threatened lizard conform to two-factor hypothesis of cryo-injury

Campbell, L., Clulow, J., Doody, J. S., & Clulow, S. (2021) · Cryobiology · doi:10.1016/j.cryobiol.2021.09.001 · Cited by 9 · see citing works

Cetacean conservation and the ethics of captivity

Muka, S. K., & Zarpentine, C. (2021) · Biological Conservation · doi:10.1016/j.biocon.2021.109303 · Cited by 9 · see citing works

A glimmer of hope for an Endangered temperate soft coral: the first observations of reproductive strategies and early life cycle of Dendronephthya australis (Octocorallia: Malacalcyonacea)

Larkin, M. F., Davis, T. R., Harasti, D., Smith, S. D. A., Ainsworth, T. D., & Benkendorff, K. (2023) · Marine Biology · doi:10.1007/s00227-023-04298-x · Cited by 8 · see citing works

The Concept of Agency, Animal Wellbeing, and the Practical Realities of Ex Situ Breeding Programs in Zoos and Aquariums

Norman, M., & Brando, S. (2024) · Journal of Zoological and Botanical Gardens · doi:10.3390/jzbg5040038 · Cited by 7 · see citing works

Resetting the paradigm of reproductive science and conservation

Rodger, J. C., & Clulow, J. (2021) · Animal Reproduction Science · doi:10.1016/j.anireprosci.2021.106911 · Cited by 7 · see citing works

Relationship between testosterone and sperm motility in Asian elephants (Elephas maximus): Potential implications during the sexual state of musth

LaDue, C. A., Brown, J. L., Davis, M., Kibe, G., & Kiso, W. K. (2024) · Theriogenology Wild · doi:10.1016/j.therwi.2024.100072 · Cited by 6 · see citing works

Hormonal induction and seasonal variation in male reproductive viability of the Southern Rocky Mountain boreal toad

Calatayud, N. E., Jacobs, L. E., Togna, G. D., Langhorne, C. J., Mullen, A. C., & Upton, R. M. O. (2024) · Animal Reproduction Science · doi:10.1016/j.anireprosci.2024.107678 · Cited by 5 · see citing works

Representations of human–elephant interactions on YouTube: Analyzing content and engagement to inform sustainable practices for wildlife tourism

LaDue, C. A., Vandercone, R. P. G., & Snyder, R. J. (2025) · Biological Conservation · doi:10.1016/j.biocon.2025.111240 · Cited by 5 · see citing works

Emerging arguments for reproductive technologies in wildlife and their implications for assisted reproduction and conservation of threatened marsupials

Howell, L. G., & Witt, R. R. (2022) · Theriogenology · doi:10.1016/j.theriogenology.2022.12.008 · Cited by 5 · see citing works

Conservation, Animal Wellbeing, and Indigenous Participation at an Elephant Sanctuary in Mondulkiri, Cambodia

Kopnina, H., & Baker, L. (2023) · Society and Animals · doi:10.1163/15685306-bja10126 · Cited by 4 · see citing works

Proteomic Insights into Seminal Plasma and Spermatozoa Proteins of Small-Spotted Catsharks, Scyliorhinus canicula: Implications for Reproductive Conservation in Aquariums

Muñoz-Baquero, M., Lorenzo-Rebenaque, L., García‐Domínguez, X., Valdés‐Hernández, J., García‐Párraga, D., Marín, C., García‐Vázquez, F. A., & Marco‐Jiménez, F. (2024) · Animals · doi:10.3390/ani14091281 · Cited by 4 · see citing works

Cryopreservation of Hydractinia symbiolongicarpus Sperm to Support Community-Based Repository Development for Preservation of Genetic Resources

Huene, A. L., Koch, J. C., Arregui, L., Liu, Y., Nicotra, M. L., Weis, V. M., & Tiersch, T. R. (2022) · Animals · doi:10.3390/ani12192537 · Cited by 4 · see citing works

Progesterone receptors in extratesticular ducts of the Amazonian stingray Potamotrygon wallacei: A potential role in sperm maturation and aggregate formation

Morales-Gamba, R. D., de Araújo, M. L. G., Barcellos, J. F. M., do Rêgo, M. G., Dias, L. C., & Marcon, J. L. (2023) · General and Comparative Endocrinology · doi:10.1016/j.ygcen.2023.114375 · Cited by 4 · see citing works

Revival of cryopreserved larvae from the important aquaculture species Pacific White Shrimp (Litopenaeus vannamei) using vitrification and ultra-rapid laser warming

Smith, K. T., Khosla, K., Han, G., Humphrey, T., Phelps, N. B. D., & Bischof, J. C. (2024) · Cryobiology · doi:10.1016/j.cryobiol.2024.105160 · Cited by 4 · see citing works

Obesity Prevalence and Associated Factors in Captive Asian Elephants (Elephas maximus) in China: A Body Condition Assessment Study

Tang, Y., Jia, T., Zhou, F., Wang, L., Chen, Z., & Zhang, L. (2024) · Animals · doi:10.3390/ani14243571 · Cited by 4 · see citing works

Of Elephants and Other Mammals: A Comparative Review of Reproductive Tumors and Potential Impact on Conservation

Abegglen, L. M., Harrison, T. M., Moresco, A., Fowles, J. S., Troan, B. V., Kiso, W. K., Schmitt, D. L., Boddy, A. M., & Schiffman, J. D. (2022) · Animals · doi:10.3390/ani12152005 · Cited by 5 · see citing works

Spawning and early-life history of the soft coral Sarcophyton cf. elegans (Octocorallia) in Okinawa Island, Japan, with notes on spawn predation by copepods

Hakim, A. A., Jamodiong, E. A., Manzano, G. G., Tabalanza, T. D., Komeda, S., Uyeno, D., Nakamura, T., Anraku, M., Kumekawa, M., Kurihara, H., et al. (2025) · Marine Biodiversity · doi:10.1007/s12526-025-01555-8 · Cited by 3 · see citing works

Protein biomarkers in serum as a conservation tool to assess reproduction: a case study on brown bears (Ursus arctos)

Wilson, A. E., Michaud, S. A., Jackson, A., Stenhouse, G. B., McClelland, C. J. R., Coops, N. C., & Janz, D. M. (2021) · Conservation Physiology · doi:10.1093/conphys/coab091 · Cited by 3 · see citing works

Nature without Suffering: Herbivorisation of Predator Species for the Compassionate Stewardship of Earth’s Ecosystems

Bruers, S., Davis, A. J., Hojjat, S., Koskensilta, A., Lepeltier, T., & Pearce, D. (2024) · Journal of Applied Animal Ethics Research · doi:10.1163/25889567-bja10051 · Cited by 4 · see citing works

Consequences of nescient mating: Artificial insemination increases cub rejection in the giant panda (Ailuropoda melanoleuca)

Li, M. F., Swaisgood, R. R., Owen, M. A., Zhang, H., Zhang, G., Qiu, J., & Martin‐Wintle, M. S. (2022) · Applied Animal Behaviour Science · doi:10.1016/j.applanim.2022.105565 · Cited by 3 · see citing works

Outcomes from an anti-gonadotropin-releasing hormone immunotherapeutic trial in large flying foxes (Pteropus vampyrus)

Greene, W., Wheaton, C. J., Daneault, R., Freeman, H. D., Rodriguez, C., Fredholm, D. V. E., Hall, N., & Mylniczenko, N. D. (2023) · American Journal of Veterinary Research · doi:10.2460/ajvr.22.10.0170 · Cited by 3 · see citing works

Vaginas

Keeffe, R., & Brennan, P. L. R. (2023) · Current Biology · doi:10.1016/j.cub.2023.05.012 · Cited by 3 · see citing works

Cryopreservation of Potamotrygon Stingrays’ Semen: Enhancing One Conservation Effort

Ramos, S. D., Jorge‐Neto, P. N., Colbachini, H., Gricio, E. A., Francisco, F. D. M., Padilha, F. L. A., Gutierrez, R. C., Requena, L. A., Reisfeld, L., Henrique, P. C., et al. (2024) · Journal of Zoological and Botanical Gardens · doi:10.3390/jzbg5020021 · Cited by 3 · see citing works

Widely Agreeable Moral Principles Support Efforts to Reduce Wild Animal Suffering

Katz, T. (2023) · Journal of Applied Animal Ethics Research · doi:10.1163/25889567-bja10038 · Cited by 3 · see citing works

Ethics at the Edge of Extinction: Assisted Reproductive Technologies (ART) in the Conservation of the Northern White Rhino

Biasetti, P., Hildebrandt, T. B., Göritz, F., Holtze, S., Stejskal, J., Galli, C., Čižmár, D., Simone, R., Seet, S., & de Mori, B. (2024) · Journal of Agricultural and Environmental Ethics · doi:10.1007/s10806-024-09943-z · Cited by 2 · see citing works

First Use of Free-Diving Photo-Identification of Porbeagle Shark (Lamna nasus) off the Brittany Coast, France

Jung, A. B. J., Ory, A., Abaut, P., & Zaccagnini, L. (2024) · Diversity · doi:10.3390/d16030155 · Cited by 2 · see citing works

Applying a heat transfer mathematical model for the cryopreservation of rainbow trout (Oncorhynchus mykiss) sperm: How straw location over liquid nitrogen level affects freezing rate and fertilization yield

Santos, M. V., Crichigno, S. A., Cussac, V. E., & Zaritzky, N. (2024) · Cryobiology · doi:10.1016/j.cryobiol.2024.104908 · Cited by 2 · see citing works

Characterisation of the urinary microbiome of a frog, and the effect of antibiotics on bacterial abundance and sperm viability during refrigerated storage

Anastas, Z. M., Byrne, P. G., & Silla, A. J. (2024) · Frontiers in Amphibian and Reptile Science · doi:10.3389/famrs.2024.1329888 · Cited by 1 · see citing works

Effects of Porcine Zona Pellucida Immunocontraception on Mare Body Condition and Foaling Season Length in Two Western Wild Horse Populations

Rutberg, A. T., & Grams, K. (2024) · Animals · doi:10.3390/ani14233550 · Cited by 1 · see citing works

Utilization of Urinary Neopterin Levels for Pregnancy Diagnosis in Mated Giant Pandas

Huang, H., Liu, Y., Kersey, D. C., Ye, Z., Hou, R., Hu, X., & Li, M. (2025) · Animals · doi:10.3390/ani15192796 · Cited by 1 · see citing works

Perceptions of assisted reproductive technologies in wildlife conservation: Public expectations and ethical implications across three EU countries

Biasetti, P., Hildebrandt, T., Seet, S., Stejskal, J., Giardullo, P., Göritz, F., Holtze, S., Galli, C., Šťastný, M., & de Mori, B. (2026) · PLoS ONE · doi:10.1371/journal.pone.0342094 · Cited by 1 · see citing works

Reproductive Characteristics of Pseudecheneis sulcatus (Siluriforms: Sisoridae) in the Lower Yarlung Zangbo River, Tibet

Lin, P., Hu, H., Gong, Z., Wang, J., & Gao, X. (2023) · Fishes · doi:10.3390/fishes8020106 · Cited by 1 · see citing works

Naive Boars and Dummy Sows: Porcine Sex and the Politics of Purity

Szczygielska, M., & Kowalewska, A. (2023) · BRILL eBooks · doi:10.1163/9789004679375_003 · Cited by 1 · see citing works

Books and Literature

Bucci, M. E. (2020) · Journal of Wildlife Management · doi:10.1002/jwmg.21819

Amphibians

Mylniczenko, N. D., & Clayton, L. A. (2023) · Elsevier eBooks · doi:10.1016/b978-0-323-83392-9.00003-4

Infant survival is significantly impacted by dam‐ and management‐related factors in zoo‐managed Eulemur populations

Robinson, C., McDonald, M. M., Stirk, J., Hoppe, P., & Ferrie, G. M. (2024) · Zoo Biology · doi:10.1002/zoo.21823

Seasonal breeding in three sympatric rodent species in semi-arid Tigray, northern Ethiopia

Welegerima, K., Makundi, R. H., Broecke, B. V., Mbije, N. E., & Meheretu, Y. (2024) · Mammalia · doi:10.1515/mammalia-2023-0100

Extracellular vesicles are involved in the paracrine communication between epithelial cells in different regions of the domestic cat epididymis

Sosnicki, D. M., Travis, A. J., & Comizzoli, P. (2024) · Biology of Reproduction · doi:10.1093/biolre/ioae129

From Pandas to Corals: Assessing the Animal Welfare Impacts of Assisted Reproduction Technologies

Sims, T., Franks, B., Ryan, E. B., & Sebo, J. (2025) · Journal of Applied Animal Ethics Research · doi:10.1163/25889567-bja10059

The use of a severity index to analyse impact of bacterial zoonoses on welfare of wildlife populations

Hirst, K., & Halsey, S. J. (2025) · Animal Welfare · doi:10.1017/awf.2025.10024

Metatheria: Marsupials

Witt, R. R., Hinds, L. A., & Rodger, J. C. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-21477-6.00425-9

Reproductive Technology - Captive Wildlife Breeding: Mammals & Birds

Penfold, L. M., & Gillis, J. D. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-21477-6.00536-8

Polymorphisms and Expression Characteristics of the ZSWIM7 Gene Are Associated with the Fertility of Male Allotetraploid of Red Crucian Carp × Common Carp

Dai, T., Dong, M., He, S., Qin, W., Yang, C., & Zhou, Y. (2026) · Animals · doi:10.3390/ani16020352

Reproductive Science as an Essential Component of Conservation Biology: New Edition

Comizzoli, P., Brown, J. L., & Holt, W. V. (2019) · Advances in experimental medicine and biology · doi:10.1007/978-3-030-23633-5_1 · Cited by 19 · see citing works

Conclusions: Environmental Change, Wildlife Conservation and Reproduction

Holt, W. V., Brown, J. L., & Comizzoli, P. (2014) · Advances in experimental medicine and biology · doi:10.1007/978-1-4939-0820-2_20 · Cited by 6 · see citing works

Reproductive Sciences in Animal Conservation Progress and Prospects

Holt, W. V., Brown, J. L., & Comizzoli, P. (2014) · Advances in experimental medicine and biology · doi:10.1007/978-1-4939-0820-2 · Cited by 20 · see citing works

Reproductive Science and Integrated Conservation

(2002) · Cambridge University Press eBooks · doi:10.1017/cbo9780511615016 · Cited by 140 · see citing works

Reproducción Animal y Biotecnologías Reproductivas en Especies Zootécnicas

Salinas, O. D. C., García, D. S. C., Mora, J. E. B., Lozano, N. V. A., Aguillón, J. R. V., Matías, J. C. V., Guadamud, W. W. A., & Masabanda, V. A. T. (2026) · Instituto de Investigaciones Transdisciplinarias Ecuador - BINARIO eBooks · doi:10.56846/bin.ec.cufu6546