Nuclear membrane-staining antinuclear antibody in patients with primary biliary cirrhosis
Abstract
An antinuclear antibody specific for nuclear membrane (ANMA) was observed by the immunofluorescence method in sera from patients with primary biliary cirrhosis (PBC). ANMA was present in 18 of 63 PBC sera (28.5) and in 1 of 431 control sera (0.2%). Its reaction appeared as a thin fluorescent ring confined to the nuclear envelope and was more evident when the sera were highly diluted and the fluorescence, due to frequently associated antimitochondrial antibody, faded. The ANMA fluorescent pattern was confirmed by indirect immunoperoxidase staining. ANMA was seen on both tissue cryostat sections and HEp-2 cells. It was a poorly or non-complement-fixing IgG, specific for an antigen resistant to DNase I, RNase, and trypsin. The significance of its presence in PBC in unknown at present. Identification of its antigen with one of the centromeric antigens is suggested.
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PBC is characterised serologically by autoantibodies specific for mitochondrial, nuclear and centromere antigens, some of which are unique to PBC.
“PBC is characterised serologically by autoantibodies specific for mitochondrial, nuclear and centromere antigens, some of which are unique to PBC.66–78”
From The British Society of Gastroenterology/UK-PBC primary biliary cholangitis treatment and management guidelines (2018) · cites this paper as [70]
They are present in ~95% (mitochondrial) and ~30% (nuclear) of patients.
“They are present in ~95% (mitochondrial) and ~30% (nuclear) of patients.”
From The British Society of Gastroenterology/UK-PBC primary biliary cholangitis treatment and management guidelines (2018) · cites this paper as [70]
Unlike in many other autoimmune diseases, these autoantibodies are, as a result of their sensitivity and specificity, extremely useful in diagnosis and have contributed significantly to the decline in the need for liver biopsy, at least for the purposes of diagnosis.
“Unlike in many other autoimmune diseases, these autoantibodies are, as a result of their sensitivity and specificity, extremely useful in diagnosis and have contributed significantly to the decline in the need for liver biopsy, at least for the purposes of diagnosis.”
From The British Society of Gastroenterology/UK-PBC primary biliary cholangitis treatment and management guidelines (2018) · cites this paper as [70]
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- Authors
- Ruffatti, A., Arslan, P., Floreani, A., Vestro, G. D. S., Calligaro, A., Naccarato, R., & Todesco, S.
- Affiliations
- University of Padua (IT)
- Azienda Ospedale - Università Padova (IT)
- Venue
- Journal of Clinical Immunology
- Published
- 1985-09
- Volume
- 5
- Issue
- 5
- Pages
- 357-361
- ISSN
- 0271-9142 (ISSN-L)
- 1573-2592
- OpenAlex
- W1964240493
- Cited by
- 45
- References
- 15
- Citations by year
- 2023: 2 · 2018: 1 · 2015: 1 · 2014: 1 · 2013: 2 · 2012: 2
- FWCI
- 1.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Journal of Clinical Immunology (publishedVersion)
- PubMed (publishedVersion)
- Padua Research Archive (University of Padova) (submittedVersion)
- Topics
- Health Sciences › Medicine › Hepatology › Liver Diseases and Immunity
- Health Sciences › Medicine › Rheumatology › Systemic Lupus Erythematosus Research
- Health Sciences › Medicine › Pathology and Forensic Medicine › Autoimmune Bullous Skin Diseases
- Keywords
- antinuclear antibodies, nuclear envelope, primary biliary cirrhosis, autoantibodies, antimitochondrial antibodies, HEp-2 cells, complement fixation, DNase I resistance
- MeSH terms
- Animals, Antibodies, Antigens, Surface, Carcinoma, Squamous Cell, Cell Line, Fluorescent Antibody Technique, Humans, Kidney, Liver, Liver Cirrhosis, Biliary, Nuclear Envelope, Staining and Labeling, Rats
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