Different in situ hybridization patterns of mitochondrial DNA in cytochrome c oxidase-deficient extraocular muscle fibres in the elderly
Abstract
Previous studies have revealed an increase of cytochrome c oxidase-deficient fibres/cells in the skeletal and heart muscle of humans during ageing. The enzyme defect is due to a lack of both mitochondrial and nuclear coded enzyme subunits. In the present investigation in situ hybridization of mitochondrial DNA (mtDNA) has been performed on extraocular muscles of humans over 70 years of age to show whether mutated mtDNA with the so called common deletion of 4,977 basepairs at position 8,482-13,460 of mtDNA accumulates in the cytochrome c oxidase-deficient fibres. The cytochrome c oxidase-deficient fibres revealed different hybridization patterns: a normal hybridization signal with three different mtDNA probes, a reduced or lacking signal with all three probes indicating depletion of mtDNA and a selective hybridization defect with the probe recognizing the "common deletion" region of mtDNA as evidence of mtDNA deletion. The results suggest that during ageing defects of cytochrome c oxidase are associated with different molecular alterations of mtDNA. Deletion and depletion of mtDNA are not the only nor probably the leading mechanisms responsible for the loss of respiratory chain capacity during ageing. The normal hybridization signal in most of the cytochrome c oxidase-deficient fibres and the loss of mitochondrial and nuclear protein subunits indicate the involvement of other, especially nuclear factors.
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Clonal expansion of mtDNA mutations has been described in numerous tissues from aged individuals, including skeletal muscle, substantia nigra neurons, heart and colonic crypts.
“Clonal expansion of mtDNA mutations has been described in numerous tissues from aged individuals, such as skeletal muscle (Fayet et al., 2002; Muller-Hocker et al., 1993), substantia nigra neurons (Cottrell et al., 2001; Kraytsberg et al., 2006), heart (Muller-Hocker, 1989) and colonic crypts (Taylor et al., 2003).”
From Role of the mitochondrial DNA replication machinery in mitochondrial DNA mutagenesis, aging and age-related diseases (2017) · cites this paper as Muller-Hocker et al., 1993
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- Authors
- Müller-Höcker, J., Seibel, P., Schneiderbanger, K., & Kadenbach, B.
- Affiliations
- Philipps University of Marburg (DE)
- Pathologisches Institut (DE)
- Venue
- Virchows Archiv A Pathological Anatomy and Histopathology
- Published
- 1993-01
- Volume
- 422
- Issue
- 1
- Pages
- 7-15
- ISSN
- 0945-6317 (ISSN-L)
- 0174-7398
- 1432-2307
- OpenAlex
- W2050821204
- Cited by
- 168
- References
- 77
- Citations by year
- 2026: 1 · 2025: 1 · 2022: 1 · 2021: 3 · 2020: 1 · 2019: 1 · 2017: 2 · 2016: 5 · 2015: 4 · 2014: 4 · 2013: 2 · 2012: 3
- FWCI
- 1.80
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Archiv für Pathologische Anatomie und Physiologie und für Klinische Medicin (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Coenzyme Q10 studies and effects
- Keywords
- cytochrome c oxidase deficiency, mitochondrial DNA, mitochondrial DNA deletions, extraocular muscles, aging, mitochondrial DNA depletion, electron transport chain, older adults
- MeSH terms
- Aged, Aging, DNA, Mitochondrial, Humans, Immunohistochemistry, Oculomotor Muscles, Staining and Labeling, Gene Deletion, In Situ Hybridization, Cytochrome-c Oxidase Deficiency
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