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Measurement of malondialdehyde by high performance liquid chromatography with fluorescence detection
Abstract
A method has been developed to measure malondial‐dehyde (MDA) in biological systems. MDA was reacted with 2‐thiobarbituric acid (TBA) in the presence of butylated hydroxytoluene (BHT) to minimize formation of artifacts. Initial separation of the TBA‐MDA adduct was accomplished by isobutanol extraction. Further elimination and separation of interfering substances was achieved by high performance liquid chromatography. The mobile phase consisted of a 1∶1 (v/v) mixture of methanol and water with 0.05% (w/v) tetrabutyl ammonium dihydrogen phosphate added as an ion pairing reagent. At a flow rate of 1 ml/min, the TBA‐MDA adduct was eluted from a 15‐cm, c‐18, reversed phase column in approximately 4.9 min. The TBA‐MDA adduct was quantitated with a fluorescence detector set at 515 nm excitation and 550 nm emission. Using this method, picomole quantities of MDA can be easily detected in plasma and liver samples.
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Cited by
Draper, H. H., Squires, E. J., Mahmoodi, H., Wu, J., Agarwal, S., & Hadley, M. (1993) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(93)90035-s · Cited by 914
Allopurinol Improves Endothelial Dysfunction in Chronic Heart Failure
Farquharson, C. A. J., Butler, R., Hill, A., Belch, J. J. F., & Struthers, A. D. (2002) · Circulation · doi:10.1161/01.cir.0000022140.61460.1d · Cited by 504
Allopurinol Normalizes Endothelial Dysfunction in Type 2 Diabetics With Mild Hypertension
Butler, R., Morris, A. D., Belch, J. J. F., Hill, A., & Struthers, A. D. (2000) · Hypertension · doi:10.1161/01.hyp.35.3.746 · Cited by 463
Trombino, S., Serini, S., Di Nicuolo, F., Celleno, L., Andò, S., Picci, N., Calviello, G., & Palozza, P. (2004) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf0303924 · Cited by 159
The inhibitory effects of coffee on radical-mediated oxidation and mutagenicity
Stadler, R. H., Turesky, R. J., Müller, O., Markovic, J., & Leong-Morgenthaler, P.-M. (1994) · Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis · doi:10.1016/0027-5107(94)90153-8 · Cited by 97
Metadata
- Authors
- Tatum, V. L., Changchit, C., & Chow, C. K.
- Venue
- Lipids
- Publisher
- Wiley
- Published
- 1990-04
- Volume
- 25
- Issue
- 4
- Pages
- 226-229
- ISSN
- 0024-4201 (ISSN-L)
- 1558-9307
- OpenAlex
- W2023632376
- Cited by
- 169
- References
- 0
- FWCI
- 5.54
- Type
- journal article
- Language
- en
- Open access
- closed
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- Tatum, V. L., Changchit, C., & Chow, C. K. (1990). Measurement of malondialdehyde by high performance liquid chromatography with fluorescence detection. Lipids. https://doi.org/10.1007/bf02535752
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
