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Measurement of malondialdehyde by high performance liquid chromatography with fluorescence detection
Abstract
A method has been developed to measure malondial‐dehyde (MDA) in biological systems. MDA was reacted with 2‐thiobarbituric acid (TBA) in the presence of butylated hydroxytoluene (BHT) to minimize formation of artifacts. Initial separation of the TBA‐MDA adduct was accomplished by isobutanol extraction. Further elimination and separation of interfering substances was achieved by high performance liquid chromatography. The mobile phase consisted of a 1∶1 (v/v) mixture of methanol and water with 0.05% (w/v) tetrabutyl ammonium dihydrogen phosphate added as an ion pairing reagent. At a flow rate of 1 ml/min, the TBA‐MDA adduct was eluted from a 15‐cm, c‐18, reversed phase column in approximately 4.9 min. The TBA‐MDA adduct was quantitated with a fluorescence detector set at 515 nm excitation and 550 nm emission. Using this method, picomole quantities of MDA can be easily detected in plasma and liver samples.
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Janero, D. R. (1990) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(90)90131-2 · Cited by 2,722
Draper, H. H., Squires, E. J., Mahmoodi, H., Wu, J., Agarwal, S., & Hadley, M. (1993) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(93)90035-s · Cited by 914
Improved analysis of malondialdehyde in human body fluids
Jentzsch, A. M., Bachmann, H., Fürst, P., & Biesalski, H. K. (1996) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(95)02043-8 · Cited by 802
Laguerre, M., Lecomte, J., & Villeneuve, P. (2007) · Progress in Lipid Research · doi:10.1016/j.plipres.2007.05.002 · Cited by 706
Allopurinol Improves Endothelial Dysfunction in Chronic Heart Failure
Farquharson, C. A. J., Butler, R., Hill, A., Belch, J. J. F., & Struthers, A. D. (2002) · Circulation · doi:10.1161/01.cir.0000022140.61460.1d · Cited by 504
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Butler, R., Morris, A. D., Belch, J. J. F., Hill, A., & Struthers, A. D. (2000) · Hypertension · doi:10.1161/01.hyp.35.3.746 · Cited by 463
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The inhibitory effects of coffee on radical-mediated oxidation and mutagenicity
Stadler, R. H., Turesky, R. J., Müller, O., Markovic, J., & Leong-Morgenthaler, P.-M. (1994) · Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis · doi:10.1016/0027-5107(94)90153-8 · Cited by 97
Metadata
- Authors
- Tatum, V. L., Changchit, C., & Chow, C. K.
- Venue
- Lipids
- Publisher
- Wiley
- Published
- 1990-04
- Volume
- 25
- Issue
- 4
- Pages
- 226-229
- ISSN
- 0024-4201 (ISSN-L)
- 1558-9307
- OpenAlex
- W2023632376
- Cited by
- 169
- References
- 0
- FWCI
- 5.54
- Type
- journal article
- Language
- en
- Open access
- closed
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