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Methods for methanolysis of sphingolipids and direct determination of long‐chain bases by gas chromatography
Abstract
A new, simple procedure is described for the direct gas chromatographic determination of sphingolipid bases as their trimethylsilyl derivatives. This method is used to study the nature of the mixture of bases produced by acidic methanolysis of ceramides, sphingomyelins, free sphingolipid bases, and N‐acetyl derivatives of the latter. Cleavage of sphingolipids with anhydrous methanolic hydrogen chloride results in the production of considerable quantities of secondary products from sphingosine, such as the O‐methyl ethers, which are not formed during methanolysis of free sphingosine. A modified reagent for methanolysis, containing methanol, water and hydrochloric acid, reduces the yield of these by‐products to low levels. Preliminary purification of the sphingolipid bases, before gas chromatography, is achieved by chromatographic procedures. Evidence is presented for the occurrence of C 16‐sphingosine in sphingomyelin from human blood plasma.
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Citations
Cited by
Shinitzky, M., & Barenholz, Y. (1974) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)42780-4 · Cited by 938
Quantitative determination of the neutral glycosyl ceramides in human blood
Vance, D. E., & Sweeley, C. C. (1967) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)38884-2 · Cited by 586
Self-citationDinkins, M. B., Dasgupta, S., Wang, G., Zhu, G., & Bieberich, E. (2014) · Neurobiology of Aging · doi:10.1016/j.neurobiolaging.2014.02.012 · Cited by 494
Ariga, T., Kohriyama, T., Freddo, L., Latov, N., Saito, M., Kon, K., Ando, S., Suzuki, M., Hemling, M. E., & Rinehart, K. L. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)75864-5 · Cited by 314
Covalently Bound Lipids of Human Stratum Corneum
Wertz, P. W., Madison, K. C., & Downing, D. T. (1989) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep13071317 · Cited by 205
Metadata
- Authors
- Gaver, R. C., & Sweeley, C. C.
- Venue
- Journal of the American Oil Chemists' Society
- Publisher
- Wiley
- Published
- 1965-04
- Volume
- 42
- Issue
- 4
- Pages
- 294-298
- ISSN
- 0003-021X (ISSN-L)
- 1558-9331
- OpenAlex
- W1964967794
- Cited by
- 533
- References
- 0
- FWCI
- 7.83
- Type
- journal article
- Language
- en
- Open access
- closed
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- Gaver, R. C., & Sweeley, C. C. (1965). Methods for methanolysis of sphingolipids and direct determination of long‐chain bases by gas chromatography. Journal of the American Oil Chemists' Society. https://doi.org/10.1007/bf02540132
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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