The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Quantitative determination of the neutral glycosyl ceramides in human blood
Abstract
A method is described for the qualitative and quantitative estimation of four neutral glycosyl ceramides from human plasma and erythrocytes. Total lipids extracted from 50 ml of plasma or packed erythrocytes were separated by silicic acid chromatography into neutral lipids, a fraction of mixed glycolipids that was eluted with acetone-methanol 9:1, and phospholipids. After mild alkali-catalyzed methanolysis to remove contaminants from the crude fraction of glycolipids, individual glycosyl ceramides were isolated by preparative thin-layer chromatography. The oligosaccharide portions of these lipids were characterized by cleavage with methanolic hydrogen chloride and gas chromatography of the O-trimethylsilyl methyl glycosides. It was possible to study the composition of the carbohydrate and sphingolipid base fractions in the same gas chromatographic analysis. With mannitol as an internal standard for gas chromatographic estimation of glucose, concentrations of each of the glycosyl ceramides were determined with a precision of about 10%. Recoveries of the lipids from plasma varied with the complexity of the oligosaccharide moiety and ranged from 94% with glucosyl ceramide to 71% with globoside. Concentrations of the four glycosyl ceramides in plasma and in erythrocytes were determined for samples from young, healthy males. Amounts of glycolipid as low as 0.1 micromole can be determined conveniently by this procedure.
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“The CMH of human plasma was comprised of both glucosyl-(GlcCer) and galactosylceramides (GalCer), as has been reported before (27)(28)(29), with higher proportions of GlcCer.”
From Lipidomics reveals a remarkable diversity of lipids in human plasma (2010) · cites this paper as [28]
Citations
Cites
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Self-citationImproved procedure for the extraction of lipids from human erythrocytes
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Self-citationCharacterization and quantification of red cell lipids in normal man
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Self-citationImproved reagent for trimethylsilylation of sphingolipid bases
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Cited by
Lipidomics reveals a remarkable diversity of lipids in human plasma
Quehenberger, O., Armando, A. M., Brown, A. H., Milne, S. B., Myers, D. S., Merrill, A. H., Bandyopadhyay, S., Jones, K. N., Kelly, S., Shaner, R. L., et al. (2010) · Journal of Lipid Research · doi:10.1194/jlr.m009449 · Cited by 1,341
Immunochemical Properties of Glycolipids and Phospholipids
Marcus, D. M., & Schwarting, G. A. (1976) · Advances in Immunology · doi:10.1016/s0065-2776(08)60321-1 · Cited by 116
Theda, C., Moser, A. B., Powers, J. M., & Moser, H. W. (1992) · Journal of the Neurological Sciences · doi:10.1016/0022-510x(92)90028-j · Cited by 99
Bizzozero, O. A., & Lees, M. B. (1986) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1986.tb13013.x · Cited by 40
Structures and fatty acid compositions of neutral glycosphingolipids of human plasma
Kościelak, J., Maśliński, W., Zieleński, J., Zdebska, E., Brudzyński, T., Miller-Podraza, H., & Cedergren, B. (1978) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(78)90158-3 · Cited by 14
Metadata
- Authors
- Vance, D. E., & Sweeley, C. C.
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 1967-11
- Volume
- 8
- Issue
- 6
- Pages
- 621-630
- ISSN
- 0022-2275
- Cited by
- 586
- References
- 35
- Language
- en
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- creativecommons.org/licenses/by/4.0/ (vor, 19332 d)
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- Vance, D. E., & Sweeley, C. C. (1967). Quantitative determination of the neutral glycosyl ceramides in human blood. Journal of Lipid Research. https://doi.org/10.1016/s0022-2275(20)38884-2
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