Plasma concentrations of glucagon during hyperglycemic clamp with or without somatostatin infusion in obese subjects
Abstract
The aim of the present study was to evaluate whether the inhibitory effect on pancreatic A-cell exerted by hyperglycemic hyperinsulinemia and/or by somatostatin administration is impaired in human obesity. For this purpose plasma glucagon concentrations were measured in 8 obese and 8 nonobese nondiabetic subjects during a 4-h hyperglycemic clamp. Synthetic cyclic somatostatin-14 was infused at the rate of 2.5 nmol/min during the third hour of the study. Fasting plasma glucagon was higher in obese than in nonobese subjects (242 +/- 32 vs 163 +/- 15 pg/ml, p less than 0.05) (mean +/- SEM). In the last 20 min of the glucose infusion period preceding somatostatin administration (100-120 min of the study) plasma glucagon averaged 195 +/- 26 pg/ml in obese and 122 +/- 13 pg/ml in nonobese subjects (p less than 0.05), with a reduction of 19 +/- 3% in the former and 28 +/- 4% in the latter (p = n.s.). In both groups somatostatin infusion did not result in a further decrease in plasma glucagon, which averaged 192 +/- 27 pg/ml in obese and 123 +/- 16 pg/ml in nonobese subjects (p less than 0.05) in the 160-180 min period of the study. Also after discontinuing somatostatin infusion plasma glucagon levels did not change. These results suggest that in human obesity hyperglycemic hyperinsulinemia has a normal inhibitory effect on pancreatic A-cell and that somatostatin administration has no additive effect on hyperglycemia and hyperinsulinemia in either obese or nonobese nondiabetic subjects.
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Surgery did not alter the inhibitory effects of glucose or insulin on α-cells.
“In our subjects, glucagon was suppressed by IV glucose in GB and control subjects alike, indicating that surgery did not alter the inhibitory effects of glucose or insulin on α-cells (26,27).”
From Gastric Bypass Surgery Enhances Glucagon-Like Peptide 1–Stimulated Postprandial Insulin Secretion in Humans (2011) · cites this paper as [26]
Cites
Glucose clamp technique: a method for quantifying insulin secretion and resistance.
DeFronzo, R. A., Tobin, J. D., & Andres, R. (1979) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1979.237.3.e214 · Cited by 7,425 · see citing works
Classification and diagnosis of diabetes mellitus and other categories of glucose intolerance.
National Diabetes Data Group (1979) · Diabetes · doi:10.2337/diab.28.12.1039 · Cited by 4,909 · see citing works
Immunoassay of insulin with insulin-antibody precipitate
Hales, C. N., & Pj, R. (1963) · Biochemical Journal · doi:10.1042/bj0880137 · Cited by 2,640 · see citing works
Studies of pancreatic alpha cell function in normal and diabetic subjects
Unger, R. H., Aguilar-Parada, E., Müller, W. A., & Eisentraut, A. M. (1970) · Journal of Clinical Investigation · doi:10.1172/jci106297 · Cited by 837 · see citing works
Insulin within islets is a physiologic glucagon release inhibitor
Maruyama, H., Hisatomi, A., Orci, L., Grodsky, G. M., & Unger, R. H. (1984) · Journal of Clinical Investigation · doi:10.1172/jci111658 · Cited by 303 · see citing works
Prager, R., Wallace, P., & Olefsky, J. M. (1986) · Journal of Clinical Investigation · doi:10.1172/jci112599 · Cited by 204 · see citing works
Blackard, W. G., Nelson, N. C., & Andrews, S. S. (1974) · Diabetes · doi:10.2337/diab.23.3.199 · Cited by 127 · see citing works
Response of circulating immunoreactive somatostatin to nutritional stimuli in normal subjects
Penman, E., Wass, J. A. H., Medbak, S., Morgan, L. D., Lewis, J. M., BESSER, G. M., & REES, L. H. (1981) · Gastroenterology · doi:10.1016/0016-5085(81)90493-5 · Cited by 116 · see citing works
Evaluation of Alpha-Cell Function by Infusion of Alanine in Normal, Diabetic and Obese Subjects
Wise, J. K., Hendler, R., & Felig, P. (1973) · New England Journal of Medicine · doi:10.1056/nejm197303082881003 · Cited by 114 · see citing works
Adrian, T. E., Barnes, A., LONG, R. G., O’Shaughnessy, D. J., Brown, M. R., Rivier, J., Vale, W., Blackburn, A. M., & Bloom, S. R. (1981) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-53-4-675 · Cited by 102 · see citing works
JIMENEZ, J., Zúñiga-Guajardo, S., Zinman, B., & Ángel, A. (1987) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-64-4-661 · Cited by 90 · see citing works
Somatostatin: Physiology and Clinical Applications
Gerich, J. E., & Patton, G. S. (1978) · Medical Clinics of North America · doi:10.1016/s0025-7125(16)31813-2 · Cited by 72 · see citing works
Bonora, E., Zavaroni, I., Bruschi, F., Alpi, O., Pezzarossa, A., GUERRA, L., Dall’Aglio, E., Coscelli, C., & BUTTURINI, U. (1984) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-59-6-1121 · Cited by 68 · see citing works
Self-citationPancreatic Somatostatin is a Mediator of Glucagon Inhibition by Hyperglycemia
Klaff, L. J., & Taborsky, G. J. (1987) · Diabetes · doi:10.2337/diab.36.5.592 · Cited by 59 · see citing works
Kalkhoff, R. K., Gossain, V. V., & Matute, M. (1973) · New England Journal of Medicine · doi:10.1056/nejm197308302890907 · Cited by 53 · see citing works
Borghi, V. C., Wajchenberg, B. L., & CESAR, F. P. (1984) · Metabolism · doi:10.1016/0026-0495(84)90089-1 · Cited by 40 · see citing works
Elevated Pancreatic Glucagon in Obesity
Starke, A. A. R., Erhardt, G., Berger, M., & Zimmermann, H. (1984) · Diabetes · doi:10.2337/diab.33.3.277 · Cited by 36 · see citing works
Role of Insulin and Glucagon in Obesity
Schade, D. S., & Eaton, R. P. (1974) · Diabetes · doi:10.2337/diab.23.8.657 · Cited by 34 · see citing works
Relative Influence of Obesity and Diabetes on Plasma Alpha-Cell Glucagon
Gossain, V. V., Matute, M., & Kalkhoff, R. K. (1974) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-38-2-238 · Cited by 33 · see citing works
Obesity: Evidence of Decreased Secretion of Glucagon
Wise, J. K., Hendler, R., & Felig, P. (1972) · Science · doi:10.1126/science.178.4060.513 · Cited by 32 · see citing works
Glucagon, insulin, and glucose responses to physiologic testing in normal and massively obese adults
Santiago, J. V., Haymond, M. W., Clarke, W. L., & Pagliara, A. S. (1977) · Metabolism · doi:10.1016/0026-0495(77)90038-5 · Cited by 29 · see citing works
Immunoreactive Glucagon Levels in Obese-hyperglycemic (ob/ob) Mice
Dubuc, P. U., Mobley, P. W., Mahler, R. J., & Ensinck, J. W. (1977) · Diabetes · doi:10.2337/diab.26.9.841 · Cited by 29 · see citing works
Souquet, J.-C., Rambliere, R., Riou, J. P., Beylot, M., Cohen, R., Mornex, R., & Chayvialle, J. A. (1983) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-56-5-1076 · Cited by 28 · see citing works
SCHUSDZIARRA, V., Lawecki, J., DITSCHUNEIT, H. H., Lukas, B., Maier, V., & Pfeiffer, E. F. (1985) · Diabetes · doi:10.2337/diab.34.6.595 · Cited by 26 · see citing works
Glucose Suppression of Glucagon: Relationship to Pancreatic Beta Cell Function?
Hatfield, H. H., BANASIAK, M. F., DRISCOLL, T., Kim, H., & Kalkhoff, R. K. (1977) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-44-6-1080 · Cited by 21 · see citing works
Glucose regulation of glucagon secretion independent of B cell activity
Asplin, C. M., Raghu, P., Dornan, T., & Palmer, J. P. (1983) · Metabolism · doi:10.1016/0026-0495(83)90195-6 · Cited by 16 · see citing works
MIHARA, S., Yanaihara, C., Nishiura, M., Ogawa, H., & Yanaihara, N. (1982) · RADIOISOTOPES · doi:10.3769/radioisotopes.31.5_240 · Cited by 10 · see citing works
Modulation of the catabolic activity of glucagon by endogenous insulin secretion in obese man
Schade, D. S., EATON, P. R., Pommer, I., & Temple, R. (1977) · Acta Diabetologica · doi:10.1007/bf02624664 · Cited by 7 · see citing works
Pancreatic glucagon response to glucose in obesity with normal and impaired carbohydrate tolerance.
Kp, R., Schulz, B., Witt, S., & Ziegler, M. (1981) · PubMed · Cited by 5 · see citing works
Erhöhte Plasma-Glucagonspiegel bei Adipositas
Starke, A. A. R., Starke, G., Jörgens, V., Berger, M., & Zimmermann, H. (2008) · DMW - Deutsche Medizinische Wochenschrift · doi:10.1055/s-2008-1070086 · Cited by 4 · see citing works
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Salehi, M., Prigeon, R. L., & D’Alessio, D. A. (2011) · Diabetes · doi:10.2337/db11-0203 · Cited by 323 · see citing works
Merino, B., Alonso‐Magdalena, P., Lluesma, M., Ñeco, P., González, A., Marroquí, L., García‐Arévalo, M., Nadal, Á., & Quesada, I. (2015) · Scientific Reports · doi:10.1038/srep11622 · Cited by 36 · see citing works
Lundqvist, M., Almby, K. E., Wiklund, U., Abrahamsson, N., Kamble, P. G., Pereira, M. J., & Eriksson, J. W. (2020) · Diabetologia · doi:10.1007/s00125-020-05332-z · Cited by 29 · see citing works
The human α cell in health and disease
Pettway, Y. D., Saunders, D. C., & Briššová, M. (2023) · Journal of Endocrinology · doi:10.1530/joe-22-0298 · Cited by 20 · see citing works
Pancreatic α‐cell mass in obesity
Ellenbroek, J. H., Töns, H. A. M., Hanegraaf, M. A. J., Rabelink, T. J., Engelse, M. A., Carlotti, F., & de Koning, E. J. P. (2017) · Diabetes Obesity and Metabolism · doi:10.1111/dom.12997 · Cited by 17 · see citing works
Fang, L., Zhang, S., Ou, K., Zuo, Z., Yu, A., & Wang, C. (2019) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2019.109875 · Cited by 12 · see citing works
Sui, H., Yi, Y., Yao, J., Liang, B., Sun, X., Hu, S., Uç, A., Nelson, D. J., Ode, K. L., Philipson, L. H., et al. (2014) · PLoS ONE · doi:10.1371/journal.pone.0090519 · Cited by 7 · see citing works
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- Authors
- Bonora, E., Moghetti, P., Cacciatori, V., Zenere, M., Tosi, F., Travia, D., Zoppini, G., Perobelli, L., & Muggeo, M.
- Affiliations
- University of Verona (IT)
- Azienda Ospedaliera Universitaria Integrata Verona (IT)
- Venue
- Acta Diabetologica Latina
- Published
- 1990-10
- Volume
- 27
- Issue
- 4
- Pages
- 309-314
- ISSN
- 0940-5429 (ISSN-L)
- 0001-5563
- 1432-5233
- OpenAlex
- W1966499297
- Cited by
- 8
- References
- 30
- Citations by year
- 2025: 1 · 2023: 1 · 2020: 1 · 2019: 1 · 2017: 1 · 2015: 1 · 2014: 1
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Acta Diabetologica (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Treatment and Management
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Keywords
- glucagon, obesity, somatostatin, hyperglycemic clamp, hyperinsulinemia, alpha cells, insulin-glucagon interaction
- MeSH terms
- Adult, Analysis of Variance, Female, Glucagon, Humans, Hyperglycemia, Male, Obesity, Somatostatin, Statistics as Topic
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