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Analytical fractionation of complex lipid mixtures: DEAE cellulose column chromatography combined with quantitative thin layer chromatography
Abstract
A quantitative chromatographic procedure for the fractionation of complex lipid mixtures is described. The method utilizes diethylaminoethyl (DEAE) cellulose column chromatography followed by thin layer chromatography (TLC). Spots produced in TLC are charred with sulfuric acid‐potassium dichromate and heat and are then measured by quantitative densitometry. Results obtained with beef brain and beef heart mitochondrial lipids are presented, and the close correspondence between column isolation procedures and the new procedure is demonstrated. Methods utilizing only column chromatography, column chromatography and TLC, and one‐ and two‐dimensional TLC without column chromatography are compared.
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Citations
Cited by
Chemistry and metabolism of lipids in the vertebrate retina
Fliesler, A. J., & Anderson, R. E. (1983) · Progress in Lipid Research · doi:10.1016/0163-7827(83)90004-8 · Cited by 1,052
[54] Thin-layer chromatography of lipids
(1969) · Methods in Enzymology · doi:10.1016/s0076-6879(69)14056-2 · Cited by 573
Quantitative densitometric thin-layer chromatography of lipids using copper acetate reagent
Fewster, M. E., Burns, B. J., & Mead, J. F. (1969) · Journal of Chromatography A · doi:10.1016/s0021-9673(00)99173-8 · Cited by 403
Lipid composition of mitochondria from bovine heart, liver, and kidney
Fleischer, S., Rouser, G., Fleischer, B., Casu, A., & Kritchevsky, G. (1967) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)40133-6 · Cited by 270
Self-citationLipid analysis by quantitative thin‐layer chromatography
Privett, O. S., Blank, M. L., Codding, D. W., & Nickell, E. C. (1965) · Journal of the American Oil Chemists' Society · doi:10.1007/bf02635573 · Cited by 172
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Metadata
- Authors
- Rouser, G., Galli, C., Lieber, E., Blank, M. L., & Privett, O. S.
- Venue
- Journal of the American Oil Chemists' Society
- Publisher
- Wiley
- Published
- 1964-12
- Volume
- 41
- Issue
- 12
- Pages
- 836-840
- ISSN
- 0003-021X (ISSN-L)
- 1558-9331
- OpenAlex
- W1984166098
- Cited by
- 210
- References
- 0
- FWCI
- 15.63
- Type
- journal article
- Language
- en
- Open access
- closed
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- Rouser, G., Galli, C., Lieber, E., Blank, M. L., & Privett, O. S. (1964). Analytical fractionation of complex lipid mixtures: DEAE cellulose column chromatography combined with quantitative thin layer chromatography. Journal of the American Oil Chemists' Society. https://doi.org/10.1007/bf02663969
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
