The DOI matches its Crossref record; 13 of the 14 references it cites were found in a registry or an index.
New strategies for the diagnosis and screening of malaria
Abstract
Thin and thick blood film microscopy are the "gold standard" for malaria diagnosis. In recent years, there have been important developments in malaria diagnostic tests including fluorescence microscopy of malaria parasites stained with acridine orange, dipstick immunoassays that detect species-specific parasite antigens, and more recently, detection of parasite nucleic acids after amplification by PCR. With some of these methods, sensitivities and specificities approaching and even exceeding those of the thin and thick film can be attained. In particular, PCR-based tests for plasmodium DNA or RNA are more sensitive and specific than other tests for malarial parasites. A specific application for PCR diagnosis of malaria could be blood donor screening. Clinical trials of blood donor sreening for malarial parasites by PCR are being conducted, in which pooled donor samples are screened to increase efficiency and reduce costs. Some of the new diagnostic methods may have specific applications in particular settings, depending on the purpose and location of testing, and other factors such as cost, desired sensitivity and specificity, speed and ease of use.
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“Also, DNA-based molecular assays have demonstrated a possible chronic detection in regions of the world where malaria is endemic, which may be due to very low parasitemias in immune individuals (3, 19, 34).”
From Real-Time PCR for Detection and Identification of Plasmodium spp (2005) · cites this paper as [19]
Citations
Cites
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Self-citationDetection of Plasmodium falciparum by Polymerase Chain Reaction in a Field Study
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Quantitative Buffy Coat Analysis
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Cited by
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Clinical review: Severe malaria.
Trampuž, A., Jereb, M., Muzlovič, I., & Prabhu, R. M. (2003) · Critical Care · doi:10.1186/cc2183 · Cited by 623
Real-Time PCR for Detection and Identification of Plasmodium spp
Mangold, K. A., Manson, R. U., Koay, E. S. C., Stephens, L., Regner, M., Thomson, R. B., Peterson, L. R., & Kaul, K. L. (2005) · Journal of Clinical Microbiology · doi:10.1128/jcm.43.5.2435-2440.2005 · Cited by 278
Self-citationNdao, M., Bandyayera, E., Kokoskin, E., Gyorkos, T. W., MacLean, J. D., & Ward, B. J. (2004) · Journal of Clinical Microbiology · doi:10.1128/jcm.42.6.2694-2700.2004 · Cited by 137
Evaluation of the RealArt Malaria LC Real-Time PCR Assay for Malaria Diagnosis
Farcas, G. A., Zhong, K. J. Y., Mazzulli, T., & Kain, K. C. (2004) · Journal of Clinical Microbiology · doi:10.1128/jcm.42.2.636-638.2004 · Cited by 87
Jiram, A. I., Ooi, C. H., Rubio, J. M., Hisam, S. R., Karnan, G., Sukor, N. M., Artic, M. M., Ismail, N. P., & Alias, N. W. (2019) · Malaria Journal · doi:10.1186/s12936-019-2786-y · Cited by 63
Metadata
- Authors
- Lee, S., Kara, U., Koay, E., Lee, M., Lam, S., & Teo, D.
- Affiliations
- National University Hospital (SG)
- ORCID
- Teo, D
- Venue
- International Journal of Hematology
- Published
- 2002-01
- Volume
- 76
- Issue
- S1
- Pages
- 291-293
- ISSN
- 0925-5710 (ISSN-L)
- 1865-3774
- OpenAlex
- W2073185482
- Cited by
- 24
- References
- 14
- Citations by year
- 2024: 2 · 2021: 1 · 2019: 2 · 2018: 1 · 2016: 1 · 2015: 2 · 2014: 2
- FWCI
- 2.09
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- International Journal of Hematology (publishedVersion)
- PubMed (publishedVersion)
- National University of Singapore (submittedVersion)
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
- Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
- Physical Sciences › Computer Science › Computer Vision and Pattern Recognition › Digital Imaging for Blood Diseases
- Keywords
- malaria diagnosis, malaria screening, blood donor screening, PCR diagnosis, dipstick immunoassay, parasite antigens, sensitivity and specificity
- MeSH terms
- Blood Donors, Humans, Malaria, Mass Screening, Polymerase Chain Reaction, Clinical Laboratory Techniques
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