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Autophagy upregulation promotes macrophages to escape mesoporous silica nanoparticle (MSN)-induced NF-κB-dependent inflammation

Xi, Chen, Zhou, Jie, Du, Shuzhang, Peng, Shaojun·2016·Inflammation Research
DOI 10.1007/s00011-016-0919-0
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Abstract

Background Our previous studies (Int J Nanomed 10:22, 2015) have indicated that a single large dose of mesoporous silica nanoparticles (MSNs) can induce severe and selective nephrotoxicity, which is closely related to inflammation mediated by the NF-κB pathway. However, the effect of MSNs on other organs and the interactions of nanomaterials with biological systems remain rudimentary. Objective This study aimed to clarify the biological behaviour and influence of MSNs on macrophages. Methods The mice received a single intraperitoneal injection of a suspension of 150, 300 of 600 mg/kg MSNs, and RAW 264.7 cells were treated with MSNs at various concentrations and times. Cell viability was determined by MTT assay and LDH release assay. The NF-κB pathway and the target proinflammatory cytokines IL-1β and TNF-α were determined by western blotting or ELISA. Autophagy is considered as an emerging mechanism of nanomaterials. So the autophagic ultrastructural analysis, the determination of Beclin-1 and LC3 expression, and the calculation of LC3II dots were employed to verify autophagy activation. In addition, RNA interference, autophagy agonist and inhibitor were used to explore the role of autophagy in inflammation. Results The results indicated that MSNs are internalized into macrophages and induce cytotoxicity in a dose- and time-dependent manner. The NF-κB pathway, IL-1β and TNF-α were induced and released by MSNs. The levels of Beclin-1 and LC3II dots were obviously up-regulated…

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Metadata

Authors
Xi, C., Zhou, J., Du, S., & Peng, S.
Venue
Inflammation Research
Publisher
Springer Science and Business Media LLC
Published
2016-02-09
Volume
65
Issue
4
Pages
325-341
ISSN
  • 1023-3830
  • 1420-908X
DOI
10.1007/s00011-016-0919-0
Cited by
48
References
1
Language
en
Licence
www.springer.com/tdm (tdm)

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CoChat. (2026). Citation check: Autophagy upregulation promotes macrophages to escape mesoporous silica nanoparticle (MSN)-induced NF-κB-dependent inflammation. https://app.cochat.ai/papers/10.1007/s00011-016-0919-0
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Xi, C., Zhou, J., Du, S., & Peng, S. (2016). Autophagy upregulation promotes macrophages to escape mesoporous silica nanoparticle (MSN)-induced NF-κB-dependent inflammation. Inflammation Research. https://doi.org/10.1007/s00011-016-0919-0