CoChat
Sign Up
Cited by 71Nitric Oxide and Endothelin EffectsDiabetes, Cardiovascular Risks, and LipoproteinsGenetic Associations and Epidemiology

Association of (−)786T–C mutation of endothelial nitric oxide synthase gene with insulin resistance

Open access
K., Ohtoshi, Y., Yamasaki, S., Gorogawa, R., Hayaishi-Okano, K., Node, M., Matsuhisa, Y., Kajimoto, M., Hori·2002·Diabetologia
DOI 10.1007/s00125-002-0922-6
Open this paper in CoChat — free accountOpen PDF

Abstract

Aims/hypothesis Endothelial derived nitric oxide synthase ( eNOS) gene polymorphisms affect eNOS activity and are associated with abnormal vasomotility and impaired local blood flow. A decrease in local blood flow has been reported to cause insulin resistance. The aim of this study was to examine a possible association of two eNOS polymorphisms, Glu298Asp (G894T) in exon 7 and (-)786T-C mutation with insulin resistance.Methods Genotypes of both Glu298Asp and (-)786T-C mutation were examined by the PCR-RFLP method. Plasma nitrate and nitrite concentrations were also measured.Results The allele frequencies of both polymorphisms showed no considerable differences in 233 non-diabetic subjects and 301 patients with Type II (non-insulin-dependent) diabetes mellitus. Non-diabetic subjects with the (-)786C allele had (p<0.05) higher fasting plasma insulin and homeostasis model assessment of insulin resistance than those with the (-)786T/ (-)786T genotype. Diabetic subjects with (-)786C allele showed higher HbA(1c) than those with the (-)786T/(-)786T genotype. A euglycaemic hyperinsulinemic clamp study done on 71 of the 301 patients showed a lower glucose infusion rate in diabetic patients with the (-)786C allele than those without it. In diabetic patients with the (-)786C allele, plasma nitrate and nitrite concentrations were lower than in subjects without it (p=0.026). No differences were observed between mutant carriers of Glu298Asp and non-carriers among both non-diabetic subjects and Type II diabetic patients.Conclusions/interpretation The (-)786T-C mutation of the eNOS gene is associated with insulin resistance in both Japanese non-diabetic subjects and Type II diabetic patients.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1007/s00125-002-0922-6

Cites

48 of 48 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Several studies have associated a T(−786)C variant of the NOS3 gene with insulin resistance.

    “Most notably, several studies have associated a T(−786)C variant of the NOS3 gene with insulin resistance [318-320].”

    From Regulation of obesity and insulin resistance by nitric oxide (2014) · cites this paper as [318]

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
K., O., Y., Y., S., G., R., H.-O., K., N., M., M., Y., K., & M., H.
Venue
Diabetologia
Publisher
Springer Science and Business Media LLC
Published
2002-11-01
Volume
45
Issue
11
Pages
1594-1601
ISSN
  • 0012-186X (ISSN-L)
  • 1432-0428
DOI
10.1007/s00125-002-0922-6
OpenAlex
W1983844941
Cited by
71
References
48
Citations by year
2024: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 3 · 2018: 1 · 2017: 3 · 2016: 2 · 2014: 4 · 2013: 3 · 2012: 7
FWCI
2.39
Type
journal article
Language
en
Open access
Licence
www.springer.com/tdm (tdm)
Locations
  • Diabetologia (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes, Cardiovascular Risks, and Lipoproteins
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Associations and Epidemiology
Keywords
endothelial nitric oxide synthase, insulin resistance, type 2 diabetes, Glu298Asp polymorphism, nitric oxide, HbA1c, glucose infusion rate, Japanese population
MeSH terms
Adult, Aged, Base Sequence, Blood Pressure, Cytidine, Diabetes Mellitus, Type 2, Exons, Female, Gene Frequency, Humans, Insulin Resistance, Japan, Male, Middle Aged, Nitrates, Nitrites, Reference Values, Thymine, Point Mutation, DNA Primers, Nitric Oxide Synthase, Polymorphism, Single Nucleotide, Asian People, Nitric Oxide Synthase Type III

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review