Prepubertal exposure to commercial formulation of the herbicide glyphosate alters testosterone levels and testicular morphology
Abstract
Glyphosate is a herbicide widely used to kill weeds both in agricultural and non-agricultural landscapes. Its reproductive toxicity is related to the inhibition of a StAR protein and an aromatase enzyme, which causes an in vitro reduction in testosterone and estradiol synthesis. Studies in vivo about this herbicide effects in prepubertal Wistar rats reproductive development were not performed at this moment. Evaluations included the progression of puberty, body development, the hormonal production of testosterone, estradiol and corticosterone, and the morphology of the testis. Results showed that the herbicide (1) significantly changed the progression of puberty in a dose-dependent manner; (2) reduced the testosterone production, in semineferous tubules' morphology, decreased significantly the epithelium height (P < 0.001; control = 85.8 +/- 2.8 microm; 5 mg/kg = 71.9 +/- 5.3 microm; 50 mg/kg = 69.1 +/- 1.7 microm; 250 mg/kg = 65.2 +/- 1.3 microm) and increased the luminal diameter (P < 0.01; control = 94.0 +/- 5.7 microm; 5 mg/kg = 116.6 +/- 6.6 microm; 50 mg/kg = 114.3 +/- 3.1 microm; 250 mg/kg = 130.3 +/- 4.8 microm); (4) no difference in tubular diameter was observed; and (5) relative to the controls, no differences in serum corticosterone or estradiol levels were detected, but the concentrations of testosterone serum were lower in all treated groups (P < 0.001; control = 154.5 +/- 12.9 ng/dL; 5 mg/kg = 108.6 +/- 19.6 ng/dL; 50 mg/dL = 84.5 +/- 12.2 ng/dL; 250 mg/kg = 76.9 +/- 14.2 ng/dL). These results suggest that commercial formulation of glyphosate is a potent endocrine disruptor in vivo, causing disturbances in the reproductive development of rats when the exposure was performed during the puberty period.
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R appears to be a sex endocrine disruptor in vivo, also in males.
“In addition, R appears to be a sex endocrine disruptor in vivo, also in males (Romano et al., 2010).”
From RETRACTED: Long term toxicity of a Roundup herbicide and a Roundup-tolerant genetically modified maize (2012) · cites this paper as Romano et al., 2010
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Glyphosate-based herbicides are toxic and endocrine disruptors in human cell lines
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“In addition, R appears to be a sex endocrine disruptor in vivo, also in males (Romano et al., 2010).”
BackgroundRetractedDefarge, N., Takács, E., Lozano, V., Mesnage, R., Spiroux de Vendômois, J., Séralini, G.-E., & Székács, A. (2016) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph13030264 · Cited by 205 · see citing works
Glyphosate impairs male offspring reproductive development by disrupting gonadotropin expression
Romano, M. A., Romano, R. M., Santos, L. D., Wisniewski, P., Campos, D. A., de Souza, P. B., Viau, P., Bernardi, M. M., Nunes, M. T., & de Oliveira, C. A. (2012) · Archives of Toxicology · doi:10.1007/s00204-011-0788-9 · Cited by 196 · see citing works
Self-citation
- Authors
- Romano, R. M., Romano, M. A., Bernardi, M. M., Furtado, P. V., & Oliveira, C. A.
- Affiliations
- Universidade de São Paulo (BR)
- Corresponding author
- Romano, R. M.
- Venue
- Archives of Toxicology
- Published
- 2009-12-12
- Volume
- 84
- Issue
- 4
- Pages
- 309-317
- ISSN
- 0340-5761 (ISSN-L)
- 1432-0738
- OpenAlex
- W2089279540
- Cited by
- 204
- References
- 45
- Citations by year
- 2026: 6 · 2025: 11 · 2024: 11 · 2023: 15 · 2022: 13 · 2021: 21 · 2020: 21 · 2019: 20 · 2018: 17 · 2017: 12 · 2016: 9 · 2015: 7 · 2014: 9 · 2013: 10 · 2012: 14
- FWCI
- 8.90
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Archives of Toxicology (publishedVersion)
- PubMed (publishedVersion)
- Scientific Electronic Library Online (São Paulo Research Foundation, Latin American and Caribbean Center on Health Sciences Information, Conselho Nacional de Desenvolvimento Científico e Tecnológico) (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Pesticide Exposure and Toxicity
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Effects and risks of endocrine disrupting chemicals
- Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
- Keywords
- glyphosate, herbicide toxicity, endocrine disruptors, reproductive toxicity, prepubertal exposure, puberty, testosterone, testicular morphology, seminiferous tubules, corticosterone, estradiol, Wistar rats
- MeSH terms
- Glyphosate, Adrenal Glands, Animals, Body Weight, Corticosterone, Environmental Exposure, Estradiol, Glycine, Herbicides, Male, Organ Size, Testis, Testosterone, Rats, Wistar, Growth and Development, Rats
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