Cannflavins isolated from Cannabis sativa impede Caenorhabditis elegans response to noxious heat
Abstract
Cannflavins, flavonoids abundantly present in Cannabis sativa, possess a distinct chemical structure comprising a vanillyl group. Notably, the capsaicin structure also contains a vanillyl group, which is considered essential for interacting with the vanilloid receptor. The vanilloid receptor plays a crucial role in the perception of pain, heat, and inflammation and mediates the analgesic effects of capsaicin. Therefore, we postulated that prolonged exposure to cannflavin A (Can A) and cannflavin B (Can B) would provoke vanilloid receptor desensitization and hinder nocifensive responses to noxious thermal stimuli. C. elegans wild-type (N2) and mutants were exposed to Can A and Can B solutions for 60 min and then aliquoted on Petri dishes divided into quadrants for thermal stimulation. We then determined the thermal avoidance index for each C. elegans experimental group. Proteomics was performed to identify proteins and pathways associated with Can A or B treatment. Prolonged exposure to Can A and Can B hindered heat avoidance (32-35 °C) in C. elegans. No antinociceptive effect was observed 6 h post Can A or B exposure. Proteomics and Reactome pathway enrichment analyses identified hierarchical differences between Can A- and B-treated nematodes. However, both treatments were related to eukaryotic translation initiation (R-CEL-72613) and metabolic processes strongly associated with pain development. Our study aids in characterizing the pharmacological activity of cannflavins isolated from Cannabis sativa and outlines a possible application as pain therapy.
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Cannflavin A and B showed anti-nociceptive activity in C. elegans heat-avoidance assay at 1 μM (1 h treatment).
“Cannflavin A and B showed anti-nociceptive activity in Caenorhabditis elegans in a heat-avoidance assay at concentrations of 1 μM (1 h treatment) [109].”
From Exploring the Lesser-Known Bioactive Natural Products of Plant Species of the Genus Cannabis L.: Alkaloids, Phenolic Compounds, and Their Therapeutic Potential (2025) · cites this paper as [109]
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- Authors
- Lahaise, M., Boujenoui, F., & Beaudry, F.
- Affiliations
- Université de Montréal (CA)
- Corresponding author
- Beaudry, Francis
- ORCID
- Beaudry, Francis
- Venue
- Naunyn-Schmiedeberg's Archives of Pharmacology
- Published
- 2023-07-22
- Volume
- 397
- Issue
- 1
- Pages
- 535-548
- ISSN
- 0028-1298 (ISSN-L)
- 1432-1912
- OpenAlex
- W4385075416
- Cited by
- 6
- References
- 77
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 1
- FWCI
- 0.94
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Naunyn-Schmiedeberg s Archives of Pharmacology (publishedVersion)
- PubMed (publishedVersion)
- Papyrus : Institutional Repository (Université de Montréal) (submittedVersion)
- Funders
- Natural Sciences and Engineering Research Council of Canada (Undergraduate Student Research Awards, RGPIN-2020-05228)
- Canada Foundation for Innovation (36706)
- Canada Research Chairs (CRC-2021-00160)
- Topics
- Health Sciences › Medicine › Pharmacology › Cannabis and Cannabinoid Research
- Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
- Health Sciences › Medicine › Physiology › Pain Mechanisms and Treatments
- Keywords
- Cannabis sativa, vanilloid receptor, TRPV1 desensitization, antinociception, pain management, Caenorhabditis elegans, eukaryotic translation initiation
- MeSH terms
- Animals, Cannabis, Capsaicin, Hot Temperature, Pain, Caenorhabditis elegans, Flavones, TRPV Cation Channels
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