Cited by 8Protein Hydrolysis and Bioactive PeptidesEchinoderm biology and ecologyAlzheimer's disease research and treatments

Characterization of β-secretase inhibitory extracts from sea cucumber (Stichopus japonicus) hydrolysis with their cellular level mechanism in SH-SY5Y cells

Ma, Eui-Hyoun, Rathnayake, A. U., Lee, Jung Kwon, Lee, Sang-Min, Byun, Hee-Guk·2021·European Food Research and Technology

Abstract

Alzheimer’s disease (AD) is the main type of dementia found in the elderly population. The prevention of β-amyloid (Aβ) production by inhibiting β-secretase, which is required for Aβ generation, is the major focus of research in AD therapeutics. In this study, enzymatic extracts obtained from sea cucumber (Stichopus japonicus) hydrolysis were investigated for β-secretase inhibitory activities. Sea cucumber hydrolysates were prepared using various enzymes (Alcalase, α-chymotrypsin, Neutrase, papain, pepsin, and trypsin). Trypsin hydrolysate (IC₅₀, 93.59 ± 0.68 μg/ml) with the highest β-secretase inhibitory activity was separated into crude polysaccharide (TCP) and protein (TP) by ethanol precipitation. TCP (IC₅₀, 16.13 ± 1.15 μg/ml) exhibited higher β-secretase inhibitory activity than trypsin hydrolysate and TP (IC₅₀, 563.50 ± 25.18 μg/ml). Total sugar content in TCP was 11.58 ± 0.32 g/100 g. Major monosaccharides present in S. japonicus were arabinose, fucose, galactose, mannose and ramnose at 1.76 mg/g, 11.98 mg/g, 1.33 mg/g and 0.1 mg/g, respectively. Sulfate and uronic acid contents were 5.69 ± 0.15 g/100 g and 0.49 ± 0.01 g/100 g. Cytotoxicity of TCP different concentrations were tested in SH-SY5Y cells using MTT assay. Treated concentrations at 1, 5, 10, 30, 50, and 100 µg/ml concentrations were not toxic at cellular level. Further, quantitative protein expression reduction of BACE, sAPPβ, β-amyloid, p-JNK, and p-p38 in SH-SY5Y cells were confirmed by Western blot analysis. Therefore, results of this study suggest the potential application of S. japonicus in food and biomedicine industries.

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Crossref · 10.1007/s00217-021-03770-6

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Authors
Ma, E.-H., Rathnayake, A. U., Lee, J. K., Lee, S.-M., & Byun, H.-G.
Affiliations
Gangneung–Wonju National University (KR)
Corresponding author
Byun, Hee-Guk
Venue
European Food Research and Technology
Published
2021-06-10
Volume
247
Issue
8
Pages
2039-2052
ISSN
  • 0373-0174 (ISSN-L)
  • 1438-2377
  • 1438-2385
OpenAlex
W3171403924
Cited by
8
References
48
Citations by year
2025: 1 · 2024: 3 · 2023: 2 · 2022: 1 · 2021: 1
FWCI
0.55
Type
journal article
Language
en
Open access
closed
Locations
European Food Research and Technology (publishedVersion)
Funders
  • Korea Institute of Marine Science and Technology promotion (20140441)
  • Ministry of Oceans and Fisheries
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Hydrolysis and Bioactive Peptides
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Echinoderm biology and ecology
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
Keywords
beta-secretase inhibition, Apostichopus japonicus, trypsin hydrolysate, polysaccharides, Alzheimer's disease, amyloid-beta, BACE, SH-SY5Y cells, monosaccharide composition, uronic acid, cytotoxicity, marine bioactive peptides

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