The DOI matches its Crossref record; 79 of the 79 references it cites were found in a registry or an index.
Advances in recombinant antibody manufacturing
Open accessAbstract
Since the first use of Chinese hamster ovary (CHO) cells for recombinant protein expression, production processes have steadily improved through numerous advances. In this review, we have highlighted several key milestones that have contributed to the success of CHO cells from the beginning of their use for monoclonal antibody (mAb) expression until today. The main factors influencing the yield of a production process are the time to accumulate a desired amount of biomass, the process duration, and the specific productivity. By comparing maximum cell densities and specific growth rates of various expression systems, we have emphasized the limiting parameters of different cellular systems and comprehensively described scientific approaches and techniques to improve host cell lines. Besides the quantitative evaluation of current systems, the quality-determining properties of a host cell line, namely post-translational modifications, were analyzed and compared to naturally occurring polyclonal immunoglobulin fractions from human plasma. In summary, numerous different expression systems for mAbs are available and also under scientific investigation. However, CHO cells are the most frequently investigated cell lines and remain the workhorse for mAb production until today.
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Fisher, A. C., Kamga, M.-H., Agarabi, C., Brorson, K., Lee, S. L., & Yoon, S. (2019) · Trends in Biotechnology · doi:10.1016/j.tibtech.2018.08.008 · Cited by 180
Animal immunization, in vitro display technologies, and machine learning for antibody discovery
Laustsen, A. H., Greiff, V., Karatt-Vellatt, A., Muyldermans, S., & Jenkins, T. P. (2021) · Trends in Biotechnology · doi:10.1016/j.tibtech.2021.03.003 · Cited by 147
Amann, T., Schmieder, V., Faustrup Kildegaard, H., Borth, N., & Andersen, M. R. (2019) · Biotechnology and Bioengineering · doi:10.1002/bit.27101 · Cited by 46
Schulze, M., Lemke, J., Pollard, D., Wijffels, R. H., Matuszczyk, J., & Martens, D. E. (2021) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2021.06.011 · Cited by 34
Metadata
- Authors
- Kunert, R., & Reinhart, D.
- Venue
- Applied Microbiology and Biotechnology
- Published
- 2016-03-03
- Volume
- 100
- Issue
- 8
- Pages
- 3451-3461
- ISSN
- 0175-7598 (ISSN-L)
- 1432-0614
- OpenAlex
- W2289965054
- Cited by
- 417
- References
- 79
- FWCI
- 20.01
- Type
- journal article
- Language
- en
- Open access
- hybrid
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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