Tunable recombinant protein expression in E. coli: Promoter systems and genetic constraints

Marschall, Lukas; Sagmeister, Patrick; Herwig, Christoph

doi:10.1007/s00253-016-8045-z

Cited by 55Microbial Metabolic Engineering and BioproductionBacterial Genetics and BiotechnologyFungal and yeast genetics research

Tunable recombinant protein expression in E. coli: Promoter systems and genetic constraints

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Marschall, Lukas, Sagmeister, Patrick, Herwig, Christoph·2017·Applied Microbiology and Biotechnology

DOI 10.1007/s00253-016-8045-zPMC PMC5566544

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Abstract

Tuning of transcription is a promising strategy to overcome challenges associated with a non-suitable expression rate like outgrowth of segregants, inclusion body formation, metabolic burden and inefficient translocation. By adjusting the expression rate-even on line-to purposeful levels higher product titres and more cost-efficient production processes can be achieved by enabling culture long-term stability and constant product quality. Some tunable systems are registered for patents or already commercially available. Within this contribution, we discuss the induction mechanisms of various Escherichia coli inherent promoter systems with respect to their tunability and review studies using these systems for expression tuning. According to the current level of knowledge, some promoter systems were successfully used for expression tuning, and in some cases, analytical evidence on single-cell level is still pending. However, only a few studies using tunable strains apply a suitable process control strategy. So far, expression tuning has only gathered little attention, but we anticipate that expression tuning harbours great potential for enabling and optimizing the production of a broad spectrum of products in E. coli.

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Protein secretion can be effectively modulated at the transcriptional level by modifying the promoters in the expression vector.
“Protein secretion can be effectively modulated at the transcriptional level by modifying the promoters in the expression vector [145].”
From Evolution of Escherichia coli Expression System in Producing Antibody Recombinant Fragments (2020) · cites this paper as [145]
The Introduction describes tuning recombinant protein expression in E. coli through promoter systems, and the prpBCDE operon section reports different expression levels with different promoter-based vectors. These passages concern protein expression, not secretion; the cited paper does not establish that modifying vector promoters effectively modulates secretion. (LLM, conf=0.86)
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New tools for recombinant protein production in <i>Escherichia coli</i>: A 5‐year update
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Authors: Marschall, L., Sagmeister, P., & Herwig, C.
Affiliations: TU Wien (AT)
Corresponding author: Herwig, Christoph
ORCID: Herwig, Christoph
Venue: Applied Microbiology and Biotechnology
Publisher: Springer Science and Business Media LLC
Published: 2016-12-21
Volume: 101
Issue: 2
Pages: 501-512
ISSN: 0175-7598 (ISSN-L)
1432-0614
DOI: 10.1007/s00253-016-8045-z
PMC: PMC5566544
OpenAlex: W2565652467
Cited by: 55
References: 108
Citations by year: 2026: 4 · 2025: 2 · 2024: 4 · 2023: 6 · 2022: 7 · 2021: 8 · 2020: 7 · 2019: 10 · 2018: 5 · 2017: 1
FWCI: 0.48
Type: journal article
Language: en
Open access: hybrid
doi.org
Licence: cc-by
creativecommons.org/licenses/by/4.0 (unspecified)
Locations: Applied Microbiology and Biotechnology (publishedVersion, cc-by)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
reposiTUm (TU Wien) (submittedVersion, cc-by)
Funders: TU Wien (TUW)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Keywords: recombinant protein expression, Escherichia coli, transcriptional regulation, inducible promoters, inclusion bodies, metabolic burden, process control, single-cell gene expression
MeSH terms: Escherichia coli, Promoter Regions, Genetic, Recombinant Proteins, Transcriptional Activation, Gene Expression Regulation, Bacterial

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Tunable recombinant protein expression in E. coli: Promoter systems and genetic constraints

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