Cited by 294Mesenchymal stem cell researchHematopoietic Stem Cell TransplantationPluripotent Stem Cells Research

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Donor age and long-term culture affect differentiation and proliferation of human bone marrow mesenchymal stem cells

Zaim, Merve, Karaman, Serap, Cetin, Guven, Isik, Sevim·2012·Annals of Hematology

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Abstract

Bone marrow-derived human mesenchymal stem cells (BM-hMSCs) represent a promising cell-based therapy for a number of degenerative conditions. Many applications require cell expansion and involve the treatment of diseases and conditions found in an aging population. Therefore, the effects of donor age and long-term passage must be clarified. In this study, the effects of donor age and long-term passage on the morphology, proliferation potential, characteristics, mesodermal differentiation ability, and transdifferentiation potential of hMSCs towards neurogenic lineage were evaluated. Cells from child donors (0-12 years, n = 6) maintained their fibroblast-like morphology up to higher passages and proliferated in a greater number than those from adult (25-50 years, n = 6) and old (over 60 years, n = 6) donors. Adipogenic, osteogenic, and neurogenic differentiation potential decreased with age, while chondrogenic potential did not change. Long-term passage affected the morphology and proliferation of hMSCs from all ages. With increasing passage number, proliferation rate decreased and cells lost their typical morphology. Expression levels of neural markers (β III tubulin and NSE) and topo II isoforms in populations of nondifferentiated hMSCs were investigated by reverse transcription polymerase chain reaction analysis. While neural marker and topo IIβ expression levels increased due to increasing passage number in adult hMSCs compared to child hMSCs, topo IIα decreased in both. These results indicated that, even under highly standardized culture conditions, donor age and long-term passage have effects on hMSC characteristics, which should be taken into account prior to stem cell-based therapies.

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Additional file 1 of Recovery of ovarian function by human embryonic stem cell-derived mesenchymal stem cells in cisplatin-induced premature ovarian failure in mice
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“However, MSCs are essential partners in organ or tissue regeneration; the availability of MSCs obtainable from donors, especially in elders, remains limited 30–32 .”
Cord-Derived Stem Cells for Pediatric Autism Spectrum Disorder: Biological Rationale and Source-Specific Trial Design
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Authors: Zaim, M., Karaman, S., Cetin, G., & Isik, S.
Affiliations: Fatih University (TR)
Şişli Etfal Eğitim ve Araştırma Hastanesi (TR)
ORCID: Zaim, Merve
Karaman, Serap
Venue: Annals of Hematology
Publisher: Springer Science and Business Media LLC
Published: 2012-03-08
Volume: 91
Issue: 8
Pages: 1175-1186
ISSN: 0939-5555 (ISSN-L)
1432-0584
DOI: 10.1007/s00277-012-1438-x
OpenAlex: W1978468353
Cited by: 294
References: 41
Citations by year: 2026: 8 · 2025: 15 · 2024: 15 · 2023: 24 · 2022: 19 · 2021: 35 · 2020: 27 · 2019: 23 · 2018: 19 · 2017: 28 · 2016: 23 · 2015: 18 · 2014: 24 · 2013: 12 · 2012: 4
FWCI: 8.84
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Annals of Hematology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
Keywords: mesenchymal stem cells, bone marrow, donor age, cellular aging, cell proliferation, multilineage differentiation, neurogenic differentiation, transdifferentiation, topoisomerase II, neural markers, stem cell therapy
MeSH terms: Adult, Age Factors, Aged, Aging, Bone Marrow Cells, Cell Differentiation, Cells, Cultured, Child, Child, Preschool, Female, Humans, Male, Middle Aged, Time Factors, Tissue Donors, Cell Proliferation, Mesenchymal Stem Cells, Primary Cell Culture

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Donor age and long-term culture affect differentiation and proliferation of human bone marrow mesenchymal stem cells

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