Cited by 294Mesenchymal stem cell researchHematopoietic Stem Cell TransplantationPluripotent Stem Cells Research

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Donor age and long-term culture affect differentiation and proliferation of human bone marrow mesenchymal stem cells

Zaim, Merve, Karaman, Serap, Cetin, Guven, Isik, Sevim·2012·Annals of Hematology

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Abstract

Bone marrow-derived human mesenchymal stem cells (BM-hMSCs) represent a promising cell-based therapy for a number of degenerative conditions. Many applications require cell expansion and involve the treatment of diseases and conditions found in an aging population. Therefore, the effects of donor age and long-term passage must be clarified. In this study, the effects of donor age and long-term passage on the morphology, proliferation potential, characteristics, mesodermal differentiation ability, and transdifferentiation potential of hMSCs towards neurogenic lineage were evaluated. Cells from child donors (0-12 years, n = 6) maintained their fibroblast-like morphology up to higher passages and proliferated in a greater number than those from adult (25-50 years, n = 6) and old (over 60 years, n = 6) donors. Adipogenic, osteogenic, and neurogenic differentiation potential decreased with age, while chondrogenic potential did not change. Long-term passage affected the morphology and proliferation of hMSCs from all ages. With increasing passage number, proliferation rate decreased and cells lost their typical morphology. Expression levels of neural markers (β III tubulin and NSE) and topo II isoforms in populations of nondifferentiated hMSCs were investigated by reverse transcription polymerase chain reaction analysis. While neural marker and topo IIβ expression levels increased due to increasing passage number in adult hMSCs compared to child hMSCs, topo IIα decreased in both. These results indicated that, even under highly standardized culture conditions, donor age and long-term passage have effects on hMSC characteristics, which should be taken into account prior to stem cell-based therapies.

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Background
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“age upon the same differentiation cue (6, 8).”
Background
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“Also the differentiation of hMSCs into neural cells [33] and endothelial cells [36] has been achieved in vitro, and the differentiation of hMSCs into cardiomyocytes in vivo and in vitro has also been discussed [37,38].”
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“claimed that donor age and long-term culture of bone marrow-derived stem cells impair the stem cells’ ability to differentiate into other mesenchymal lineages [26].”
Background
The Effect of Culture on Human Bone Marrow Mesenchymal Stem Cells: Focus on DNA Methylation Profiles
Bentivegna, A., Roversi, G., Riva, G., Paoletta, L., Redaelli, S., Miloso, M., Tredici, G., & Dalprà, L. (2016) · Stem Cells International · doi:10.1155/2016/5656701 · Cited by 24
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Background
Generation and miRNA Characterization of Equine Induced Pluripotent Stem Cells Derived from Fetal and Adult Multipotent Tissues
Pessôa, L. V. D. F., Pires, P. R. L., del Collado, M., Pieri, N. C. G., Recchia, K., Souza, A. F., Perecin, F., da Silveira, J. C., de Andrade, A. F. C., Ambrosio, C. E., et al. (2019) · Stem Cells International · doi:10.1155/2019/1393791 · Cited by 29
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“However, BMSCs collected from the elderly are not entirely suitable owing to their limited ability to differentiate and low rate of proliferation (Pittenger et al., 1999; Zaim et al., 2012).”
Background
iPSC-derived mesenchymal stem cells attenuate cerebral ischemia-reperfusion injury by inhibiting inflammatory signaling and oxidative stress
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“However, comparisons of the biological characteristics between BMSCs and ADSCs have been controversial; this is because many factors, including health status and aging, can influence the biological characteristics of these cell types [15,16].”
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“Moreover, the proliferation ability and differentiation potential of human bone marrow-derived MSCs (hBM-MSCs) decrease with age [12,13].”
Background
FAM96B inhibits the senescence of dental pulp stem cells
Liang, H., Li, W., Yang, H., Cao, Y., Ge, L., Shi, R., Fan, Z., Dong, R., & Zhang, C. (2020) · Cell Biology International · doi:10.1002/cbin.11319 · Cited by 21
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“The advantage of bone marrow-derived allogeneic cells is the possibility of cryopreservation and higher potential for regeneration and immunomodulation, when isolated from young healthy donors [22,32].”
Background
Age-Related Alterations Affecting the Chondrogenic Differentiation of Synovial Fluid Mesenchymal Stromal Cells in an Equine Model
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“Indeed, multiple influences of age on stem cell function have been confirmed, including effects on stem cell number, proliferation potential, senescence and apoptosis, replicative capacity, and in vitro differentiation potential [30].”
Background
Maintenance of Neurogenic Differentiation Potential in Passaged Bone Marrow-Derived Human Mesenchymal Stem Cells Under Simulated Microgravity Conditions
Koaykul, C., Kim, M., Kawahara, Y., Yuge, L., & Kino‐oka, M. (2019) · Stem Cells and Development · doi:10.1089/scd.2019.0146 · Cited by 22
“…expansion under conventional gravity culture conditions, cells with an increased passage number can lose their abilities of proliferation and potency of multilineage differentiation [4–6], along with a reduction in neurogenic gene expressions in the late-passaged differentiated neural cells [7].”
Mesenchymal stem cells derived from iPSCs expressing interleukin-24 inhibit the growth of melanoma in the tumor-bearing mouse model
Wu, Z., Liu, W. Y., Wang, Z., Zeng, B., Peng, G., Niu, H., Chen, L., Liu, C., Hu, Q., Zhang, Y., et al. (2020) · Cancer Cell International · doi:10.1186/s12935-020-1112-7 · Cited by 20
Genetic profiling of human bone marrow and adipose tissue-derived mesenchymal stem cells reveals differences in osteogenic signaling mediated by graphene
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Connective Tissue Growth Factor reporter mice label a subpopulation of mesenchymal progenitor cells that reside in the trabecular bone region
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“Nevertheless, scalability remains limited because successive replications of MSCs and long-term culture to acquire large quantities of cells may affect their properties (Lee et al., 2009; Zaim et al., 2012).”
Background
New insights into the immunomodulatory role of exosomes in cardiovascular disease
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“However, major concerns such as the formation of teratomas (Jeong et al., 2011), immune rejection (Lalit et al., 2014), risk of embolization (Furlani et al., 2009), and low survival (Zaim et al., 2012) have hindered their clinical application.”
Background
Downregulated CXCL12 expression in mesenchymal stem cells associated with severe aplastic anemia in children
Chao, Y., Wu, K., Chiou, S.-H., Chiang, S., Huang, K. C., Yang, H.-C., Chan, C.-K., Peng, C., Wu, H., Chow, K., et al. (2014) · Annals of Hematology · doi:10.1007/s00277-014-2159-0 · Cited by 19
Increased proliferation and differentiation capacity of placenta-derived mesenchymal stem cells from women of median maternal age correlates with telomere shortening
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“years) [23] possibly due to the source of MSCs [32].”
BackgroundResultInfluential
Effect of Donor Age on the Proportion of Mesenchymal Stem Cells Derived from Anterior Cruciate Ligaments
Lee, D., Ng, J., Kim, S.-B., Sonn, C. H., Lee, K., & Han, S.-B. (2015) · PLoS ONE · doi:10.1371/journal.pone.0117224 · Cited by 18
“1 × 10(5) P1 cells were seeded into a 100-mm diameter culture dish containing 10 mL of MSCGM, incubated in 37°C, 5% CO2 incubator, and harvested after 7 days to get P2 cells[16,17].”
Method
Quality Evaluation of Human Bone Marrow Mesenchymal Stem Cells for Cartilage Repair
Shiraishi, K., Kamei, N., Takeuchi, S., Yanada, S., Mera, H., Wakitani, S., Adachi, N., & Ochi, M. (2017) · Stem Cells International · doi:10.1155/2017/8740294 · Cited by 19
“There have been reports that differentiation and proliferation capacity decrease with age [14, 15] and, consequently, the use of autologous MSCs for tissue repair, which in some indications concerns elderly patients, has certain limits [16].”
Background
Common and different alterations of bone marrow mesenchymal stromal cells in myelodysplastic syndrome and multiple myeloma
Choi, H., Kim, Y., Kang, D., Kwon, A., Kim, J., Kim, J. M., Park, S., Kim, Y., Min, C., & Kim, M. (2020) · Cell Proliferation · doi:10.1111/cpr.12819 · Cited by 18
“MM12 76 F MM IgA(κ) 31 42,X,-X,i(1)(q10),i(5)(p10),i(9)(q10),-13,-14,-16,add(22)(q13)[21]/46,XX[9] 4”
Autologous stem cell transplantation for patients with viral hepatitis-induced liver cirrhosis: a systematic review and meta-analysis
Chen, B., Pang, L., Cao, H., Wu, D., Wang, Y., Tao, Y., Wang, M., & Chen, E. (2019) · European Journal of Gastroenterology & Hepatology · doi:10.1097/meg.0000000000001455 · Cited by 14
Comparison of the Characteristics of Breast Milk-derived Stem Cells with the Stem Cells Derived from the Other Sources: A Comparative Review
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Human Bone Marrow-Derived Mesenchymal Stem Cell Applications in Neurodegenerative Disease Treatment and Integrated Omics Analysis for Successful Stem Cell Therapy
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Immunomodulatory Effects of Mesenchymal Stromal Cells in Crohn’s Disease
Molendijk, I., Duijvestein, M., Jong, A. E. V. D. M., van Deen, W. K., Swets, M., Hommes, D. W., & Verspaget, H. W. (2012) · Journal of Allergy · doi:10.1155/2012/187408 · Cited by 17
“[65] M.”
Background
DNA topoisomerase IIβ stimulates neurite outgrowth in neural differentiated human mesenchymal stem cells through regulation of Rho-GTPases (RhoA/Rock2 pathway) and Nurr1 expression
Zaim, M., & Işık, S. (2018) · Stem Cell Research & Therapy · doi:10.1186/s13287-018-0859-4 · Cited by 17
“On the other hand, increasing donor age accelerates changes in hMSC morphology, and cells become enlarged in the culture even at early passages [31].”
Self-citation
DONOR VARIABILITY IN HUMAN MESENCHYMAL STEM CELL OSTEOGENIC RESPONSE AS A FUNCTION OF PASSAGE CONDITIONS AND DONOR SEX
Kolliopoulos, V., Tiffany, A., Polanek, M., & Harley, B. A. C. (2023) · doi:10.1101/2023.11.12.566781 · Cited by 17
Characterization of Induction and Targeting of Senescent Mesenchymal Stromal Cells
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“9 To leverage the therapeutic potential of autologous cells, it is essential to develop effective methods that target and instruct MSCs for improved bone formation in the elderly.”
Biology and therapeutic potential of mesenchymal stem cell extracellular vesicles in axial spondyloarthritis
Tavasolian, F., & Inman, R. D. (2023) · Communications Biology · doi:10.1038/s42003-023-04743-z · Cited by 17
Pooling umbilical cord-mesenchymal stromal cells derived from selected multiple donors reduces donor-dependent variability and improves their immunomodulatory properties
Mebarki, M., Moine-Picard, C., Enjaume-Rauch, R., Laurent-Puig, A., Suissa, A., Feyants, V., Larghero, J., & Cras, A. (2025) · Stem Cell Research & Therapy · doi:10.1186/s13287-025-04361-y · Cited by 16
“Indeed, the extensive ex vivo expansion over several passages may introduce the risk of genetic instability and chromosomal abnormalities as well as an alteration of the biological properties of the MSCs [42].”
The Variable Role of SIRT1 in the Maintenance and Differentiation of Mesenchymal Stem Cells
Zainabadi, K. (2018) · Regenerative Medicine · doi:10.2217/rme-2017-0128 · Cited by 18
Optimizing In Vitro Osteogenesis in Canine Autologous and Induced Pluripotent Stem Cell-Derived Mesenchymal Stromal Cells with Dexamethasone and BMP-2
Gasson, S. B., Dobson, L. K., Chow, L., DOW, S. W., Gregory, C. A., & Saunders, W. B. (2020) · Stem Cells and Development · doi:10.1089/scd.2020.0144 · Cited by 16
A Preclinical Study of Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells for Treating Detrusor Underactivity by Chronic Bladder Ischemia
Yu, H. Y., Shin, J. H., Yun, H., Ryu, C., Lee, S., Heo, J., Lim, J., Park, J., Hong, K., Chung, H., et al. (2021) · Stem Cell Reviews and Reports · doi:10.1007/s12015-021-10204-z · Cited by 16
Expression of tenocyte lineage-related factors from tonsil-derived mesenchymal stem cells
Yu, Y., Lee, S. Y., Yang, E.-J., Kim, H. Y., Jo, I., & Shin, S.-J. (2016) · Tissue Engineering and Regenerative Medicine · doi:10.1007/s13770-016-9134-x · Cited by 16
“Furthermore, donor age may also affect the differentiation potential of MSCs [26-28].”
From skeletal development to the creation of pluripotent stem cell-derived bone-forming progenitors
Tam, W. L., Luyten, F. P., & Roberts, S. J. (2018) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2017.0218 · Cited by 15
Correlation between in vitro expansion-related cell stiffening and differentiation potential of human mesenchymal stem cells
LeBlon, C. E., Casey, M. E., Fodor, C. R., Zhang, T., Zhang, X. H., & Jedlicka, S. S. (2015) · Differentiation · doi:10.1016/j.diff.2015.08.002 · Cited by 15
An in Vitro and in Vivo Study of the Effect of Dexamethasone on Immunoinhibitory Function of Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells
Wang, D., Sun, Y., Gao, W., Fan, X., Shi, J., & Fu, Q. (2018) · Cell Transplantation · doi:10.1177/0963689718780194 · Cited by 15
Recombinant expression a novel fibronectin—collage fusion peptide modulating stem cell stemness via integrin β3
Luo, X., Geng, D., Zhang, Q., Ye, T., Zhang, Y., Li, Z., Huang, Y., & Xiang, Q. (2022) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-022-11965-4 · Cited by 15
“However, multiple passages of in vitro cultivation gradually decrease the therapeutic properties of stem cells by weakening their proliferation and multipotent differentiation potential, eventually leading to treatment failure (Truong et al. 2019; Zaim et al. 2012).”
Background
Donor Sex and Passage Conditions Influence MSC Osteogenic Response in Mineralized Collagen Scaffolds
Kolliopoulos, V., Tiffany, A. S., Polanek, M., & Harley, B. A. C. (2024) · Advanced Healthcare Materials · doi:10.1002/adhm.202400039 · Cited by 32
Treatment of Osteochondritis Dissecans of the Talus in Skeletally Immature Population
Buda, R., Pagliazzi, G., Castagnini, F., Cavallo, M., & Giannini, S. (2016) · Foot & Ankle Specialist · doi:10.1177/1938640016640889 · Cited by 14
“The use of undifferentiated cells could probably achieve the best outcome, since multipotent cells have an high capability of proliferating and differentiating in younger people.(25) Although pediatric treatments require very long follow-ups to be judged appropriate, another major critical point is the lack of long-te…”
Background
Nrf2 modulates immunosuppressive ability and cellular senescence of human umbilical cord mesenchymal stem cells
Zhang, J., Yao, H., Wu, M., Li, Y., Huafeng, P., & Yang, K. (2020) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2020.03.175 · Cited by 14
Research Progress on Strategies that can Enhance the Therapeutic Benefits of Mesenchymal Stromal Cells in Respiratory Diseases With a Specific Focus on Acute Respiratory Distress Syndrome and Other Inflammatory Lung Diseases
Enes, S. R., Krasnodembskaya, A. D., English, K., dos Santos, C. C., & Weiss, D. J. (2021) · Frontiers in Pharmacology · doi:10.3389/fphar.2021.647652 · Cited by 15
“It is well known that MSC properties are affected by factors such as donor age, gender, and harvest site (Zaim et al., 2012; Martin et al., 2016; Rolandsson Enes et al., 2016; Mehrian et al., 2020).”
Background
Multinuclear MRI Reveals Early Efficacy of Stem Cell Therapy in Stroke
Helsper, S., Yuan, X., Bagdasarian, F. A., Athey, J., Li, Y., Borlongan, C. V., & Grant, S. C. (2022) · Translational Stroke Research · doi:10.1007/s12975-022-01057-w · Cited by 14
“Indeed, significant work in the literature has been dedicated to evaluating therapeutic variability based on donor variance [10–12] and/or cell handing procedures [13, 14].”
Background
A bone matrix‐simulating scaffold to alleviate replicative senescence of mesenchymal stem cells during long‐term expansion
Su, X., Jing, H., Yu, W., Lei, F., Wang, R., Hu, C., Li, M., Lin, T., Zhou, H., Wang, F., et al. (2020) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.36958 · Cited by 13
Restoration of FVIII Function and Phenotypic Rescue in Hemophilia A Mice by Transplantation of MSCs Derived From F8-Modified iPSCs
Qiu, L., Xie, M., Zhou, M., Liu, X., Hu, Z., & Wu, L. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.630353 · Cited by 13
“…used in preclinical studies and clinical trials are mainly isolated from bone marrow and adipose tissue; these sources may be limited in a single donor and may cause heterogeneity (Phinney et al., 1999; Pevsner-Fischer et al., 2011; Zaim et al., 2012; McLeod and Mauck, 2017; Huang et al., 2019).”
Background
Characterization and Angiogenic Potential of Human Neonatal and Infant Thymus Mesenchymal Stromal Cells
Wang, S., Mundada, L. V., Johnson, S., Wong, J. H., Witt, R. G., Ohye, R. G., & Si, M. (2015) · Stem Cells Translational Medicine · doi:10.5966/sctm.2014-0240 · Cited by 14
Chorionic villi derived mesenchymal like stem cells and expression of embryonic stem cells markers during long-term culturing
Katsiani, E., Garas, A., Skentou, C., Tsezou, A., Messini, C. I., Dafopoulos, K. C., Daponte, A. I., & Messinis, I. (2016) · Cell and Tissue Banking · doi:10.1007/s10561-016-9559-4 · Cited by 13
The effect of medium composition on deposition of collagen type 1 and expression of osteogenic genes in mesenchymal stem cells derived from human adipose tissue and bone marrow
Szöke, K., Daňková, J., Buzgo, M., Amler, E., Brinchmann, J. E., & Østrup, E. (2016) · Process Biochemistry · doi:10.1016/j.procbio.2016.10.011 · Cited by 12
Non-Genomic Hallmarks of Aging—The Review
Holmannová, D., Borský, P., Kovaříková, H., Štveráková, T., Vošmik, M., Hruška, L., Fiala, Z., & Borská, L. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms242015468 · Cited by 13
“They found that the potential for adipogenic, osteogenic and neurogenic differentiation declines with age, whereas chondrogenic differentiation is preserved [170].”
Background
Human hair follicle-derived mesenchymal stem cells improve ovarian function in cyclophosphamide-induced POF mice
Mo, J., Hu, H., Li, P., Yang, Y., Chen, W., Chen, L., Qiao, J., Zhao, X., Yan, Q., & Chen, C. (2025) · Stem Cell Research & Therapy · doi:10.1186/s13287-024-04097-1 · Cited by 12
“They also have a limited ability for proliferative senescence and are more likely to be pro-inflammatory when generated from older donors [31– 33].”
Harnessing stem cell therapeutics in LPS-induced animal models: mechanisms, efficacies, and future directions
Wang, C., Ge, F., Ge, F., Xu, Z., & Jiang, J. (2025) · Stem Cell Research & Therapy · doi:10.1186/s13287-025-04290-w · Cited by 13
“Donor age can also affect the proliferation and differentiation potential of MSCs, with MSCs from younger donors exhibiting less damage and better proliferation [108].”
Autologous Bone Marrow Stem Cell Transplantation in Patients with Liver Failure: A Meta-Analytic Review
Wang, K., Chen, X., & Ren, J. (2014) · Stem Cells and Development · doi:10.1089/scd.2014.0337 · Cited by 12
“Age, an important confounding factor for liver failure, is associated with severity of clinical complications and functional activity of stem cells [41–43].”
Heterogeneity of baseline neural marker expression by undifferentiated mesenchymal stem cells may be correlated to donor age
Heng, B. C., Saxena, P., & Fussenegger, M. (2014) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2014.01.021 · Cited by 12
Polymer-Coated Surface as an Enzyme-Free Culture Platform to Improve Human Mesenchymal Stem Cell (hMSC) Characteristics in Extended Passaging
Choi, G., Cho, Y., Yu, S. J., Baek, J., Lee, M.-S., Kim, Y., Lee, E., & Im, S. G. (2020) · ACS Applied Bio Materials · doi:10.1021/acsabm.0c00844 · Cited by 12
From Transcriptome to Behavior: Intranasal Injection of Late Passage Human Olfactory Stem Cells Displays Potential in a Rat Model of Parkinson’s Disease
Alizadeh, R., Boroujeni, M. E., Kamrava, S. K., Tehrani, A. M., Bagher, Z., Heidari, F., Bluyssen, H. A. R., & Farhadi, M. R. (2021) · ACS Chemical Neuroscience · doi:10.1021/acschemneuro.1c00225 · Cited by 12
Aged human mesenchymal stem cells: the duration of bone morphogenetic protein-2 stimulation determines induction or inhibition of osteogenic differentiation
Heggebö, J., Haasters, F., Polzer, H. W., Schwarz, C., Saller, M. M., Mutschler, W., Schieker, M., & Prall, W. C. (2014) · Orthopedic Reviews · doi:10.4081/or.2014.5242 · Cited by 13
Multiple treatments with human embryonic stem cell-derived mesenchymal progenitor cells preserved the fertility and ovarian function of perimenopausal mice undergoing natural aging
Shin, E., Jeong, S., Lee, J. E., Jeong, D. S., Han, D. K., Hong, S., & Lee, D. R. (2024) · Stem Cell Research & Therapy · doi:10.1186/s13287-024-03684-6 · Cited by 11
“Furthermore, MPCs have a limited cell growth capacity in culture; therefore, it is difficult to obtain sufficient quantities for clinical use [8–10].”
Background
Female Age Affects the Mesenchymal Stem Cell Characteristics of Aspirated Follicular Cells in the In Vitro Fertilization Programme
Virant‐Klun, I., Omejec, S., Štimpfel, M., Škerl, P., Novaković, S., Jančar, N., & Bokal, E. V. (2019) · Stem Cell Reviews and Reports · doi:10.1007/s12015-019-09889-0 · Cited by 11
Mesenchymal stromal cell therapy for coronavirus disease 2019: which? when? and how much?
Shahani, P., & Datta, I. (2021) · Cytotherapy · doi:10.1016/j.jcyt.2021.04.004 · Cited by 12
“Christy et al. [100] showed that although AD-MSCs and BM-MSCs both expressed TF/CD142, the expression varied drastically between donors and population doublings, indicating a pro-coagulant phenotype.”
Influential
Epigenetic Analysis and Suitability of Amniotic Fluid Stem Cells for Research and Therapeutic Purposes
Phermthai, T., Suksompong, S., Tirawanchai, N., Issaragrisil, S., Julavijitphong, S., Wichitwiengrat, S., Silpsorn, D., & Pokathikorn, P. (2012) · Stem Cells and Development · doi:10.1089/scd.2012.0371 · Cited by 11
“Previous studies have reported the loss of differentiation potential in stem cells cultured for extended passages derived from various sources, such as embryonic stem cells [4], MSCs obtained from adipose [5], and bone marrow [6].”
Orthogonal potency analysis of mesenchymal stromal cell function during ex vivo expansion
Khong, D., Li, M., Singleton, A., Chin, L.-Y., Mukundan, S., & Parekkadan, B. (2017) · Experimental Cell Research · doi:10.1016/j.yexcr.2017.11.007 · Cited by 11
Mesenchymal Endometrial Stem/Stromal Cells for Hard Tissue Engineering: A Review of in Vitro and in Vivo Evidence
Tabatabaei, F. S., & Ai, J. (2017) · Regenerative Medicine · doi:10.2217/rme-2017-0029 · Cited by 11
“Therefore, identification of other sources of MSC in hard tissue engineering could help to sorting out these difficulties [19,20].”
Human umbilical cord-derived mesenchymal stromal cells for the treatment of steroid refractory grades III-IV acute graft-versus-host disease with long-term follow-up
Niu, J.-W., Li, Y., Chen, X., Sheng, H., Tian, C., Ning, H., Hu, J., Chen, J., Li, B., Wang, J., et al. (2024) · Frontiers in Immunology · doi:10.3389/fimmu.2024.1436653 · Cited by 10
Metformin acts on miR-181a-5p/PAI-1 axis in stem cells providing new strategies for improving age-related osteogenic differentiation decline
Hong, G., Zhou, Y., Yang, S., Yan, S., Lu, J., Xu, B., Zhan, Z., Jiang, H.-S., Wei, B., & Wang, J. (2024) · Stem Cells · doi:10.1093/stmcls/sxae057 · Cited by 10
Mesenchymal stromal cell therapies for traumatic neurological injuries
Wang, X., Wang, Q., Xia, Z., Yang, Y., Dai, X., Zhang, C., Wang, J., & Xu, Y. (2024) · Journal of Translational Medicine · doi:10.1186/s12967-024-05725-3 · Cited by 10
“The collection of BM requires an invasive procedure and the age of the BM donor affects the proliferation rate and long-term differentiation capacity of BM-MSC [101].”
Secreted matrix metalloproteinase-14 is a predictor for antifibrotic effect of IC-2-engineered mesenchymal stem cell sheets on liver fibrosis in mice
Fukushima, K., Itaba, N., Kono, Y., Okazaki, S., Enokida, S., Kuranobu, N., Murakami, J., Enokida, M., Nagashima, H., Kanzaki, S., et al. (2021) · Regenerative Therapy · doi:10.1016/j.reth.2021.08.004 · Cited by 11
“It is generally supposed that the quality of MSCs, assessed by proliferative and differentiation abilities, declines with age [46,47].”
Background
Proof of Concept on Functionality Improvement of Mesenchymal Stem-Cells, in Postmenopausal Osteoporotic Women Treated with Teriparatide (PTH1-34), After Suffering Atypical Fractures
Casado‐Díaz, A., Dorado, G., Giner, M., Montoya-García, M.-J., Navarro-Valverde, C., Díez‐Pérez, A., & Quesada‐Gómez, J. M. (2019) · Calcified Tissue International · doi:10.1007/s00223-019-00533-0 · Cited by 10
Hypoxic Preconditioning as a Strategy to Maintain the Regenerative Potential of Mesenchymal Stem Cells
Bahir, B., Choudhery, M. S., & Hussain, I. (2020) · IntechOpen eBooks · doi:10.5772/intechopen.93217 · Cited by 12
Higher Levels of Circulating Osteoprogenitor Cells Are Associated With Higher Bone Mineral Density and Lean Mass in Older Adults: A Cross‐Sectional Study
Feehan, J., Smith, C., Tripodi, N., Degabrielle, E., Saedi, A. A., Vogrin, S., Duque, G., & Levinger, I. (2021) · JBMR Plus · doi:10.1002/jbm4.10561 · Cited by 10
“Age-related deterioration of MPCs leads to reduced proliferation,((3)) diminished osteogenesis,((4)) and an increased tendency toward adipogenic differentiation,((5)) with detrimental effects on musculoskeletal health such as onset of frailty, osteoporosis, and sarcopenia.”
Background
Stem cell-based hydrogel for the repair and regeneration of cartilage
Yao, Z., You, X., Wang, X., Kang, Y., Kang, Y., & Zhang, Z. (2024) · Chinese Chemical Letters · doi:10.1016/j.cclet.2024.110607 · Cited by 9
Effects of Ginsenoside Rg1 on the Biological Behavior of Human Amnion-Derived Mesenchymal Stem/Stromal Cells (hAD-MSCs)
Li, L., Han, S., Huang, Y., Hou, J., & Hua, Y. (2023) · Stem Cells International · doi:10.1155/2023/7074703 · Cited by 9

Authors: Zaim, M., Karaman, S., Cetin, G., & Isik, S.
Affiliations: Fatih University (TR)
Şişli Etfal Eğitim ve Araştırma Hastanesi (TR)
ORCID: Zaim, Merve
Karaman, Serap
Venue: Annals of Hematology
Publisher: Springer Science and Business Media LLC
Published: 2012-03-08
Volume: 91
Issue: 8
Pages: 1175-1186
ISSN: 0939-5555 (ISSN-L)
1432-0584
DOI: 10.1007/s00277-012-1438-x
OpenAlex: W1978468353
Cited by: 294
References: 41
Citations by year: 2026: 8 · 2025: 15 · 2024: 15 · 2023: 24 · 2022: 19 · 2021: 35 · 2020: 27 · 2019: 23 · 2018: 19 · 2017: 28 · 2016: 23 · 2015: 18 · 2014: 24 · 2013: 12 · 2012: 4
FWCI: 8.84
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Annals of Hematology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
Keywords: mesenchymal stem cells, bone marrow, donor age, cellular aging, cell proliferation, multilineage differentiation, neurogenic differentiation, transdifferentiation, topoisomerase II, neural markers, stem cell therapy
MeSH terms: Adult, Age Factors, Aged, Aging, Bone Marrow Cells, Cell Differentiation, Cells, Cultured, Child, Child, Preschool, Female, Humans, Male, Middle Aged, Time Factors, Tissue Donors, Cell Proliferation, Mesenchymal Stem Cells, Primary Cell Culture

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Donor age and long-term culture affect differentiation and proliferation of human bone marrow mesenchymal stem cells

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