Cited by 68Antimicrobial Peptides and ActivitiesPlant-Microbe Interactions and ImmunityTransgenic Plants and Applications

Expression of the antimicrobial peptides in plants to control phytopathogenic bacteria and fungi

Oard, S. V., Enright, F. M.·2006·Plant Cell Reports

Abstract

Three antimicrobial peptides exhibiting in vitro antifungal activity were expressed in Arabidopsis to compare their in planta activity. Beta-Purothionin, cecropin B, and phor21 were expressed under an endogenous promoter with moderate-level activity and excreted extracellularly. Expression of beta-purothionin rendered the greatest antibacterial and antifungal resistance while cecropin B enhanced only antibacterial activity and phor21 did not improve antimicrobial resistance. The transgenic beta-purothionin arrested fungal growth on leaf surfaces and infection of stomata. Leaf extracts from plants producing beta-purothionin and cecropin B displayed membrane permeabilizing activity. The in planta antimicrobial activity of the tested peptides was consistent with previously reported in vitro experiments. The expression strategy allowed enhanced antifungal resistance without high-level transgene expression.

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Crossref · 10.1007/s00299-005-0102-5

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  • Antimicrobial peptides could be expressed from a single heterologous gene, as has been shown in the nucleus and chloroplast of plants.

    “As an alternative, antimicrobial peptides (AMPs) could be expressed from a single heterologous gene, as has been shown in the nucleus and chloroplast of plants [74,75].”

    From Biofuels from algae: Challenges and potential (2010) · cites this paper as [75]

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Authors
Oard, S. V., & Enright, F. M.
Affiliations
Louisiana State University (US)
Corresponding author
Oard, S. V.
Venue
Plant Cell Reports
Published
2006-02-03
Volume
25
Issue
6
Pages
561-572
ISSN
  • 0721-7714 (ISSN-L)
  • 1432-203X
OpenAlex
W2054434785
Cited by
68
References
44
Citations by year
2026: 2 · 2025: 4 · 2024: 2 · 2023: 4 · 2022: 3 · 2021: 2 · 2020: 3 · 2019: 3 · 2018: 4 · 2017: 2 · 2016: 1 · 2015: 2 · 2014: 5 · 2013: 3 · 2012: 8
FWCI
3.26
Type
journal article
Language
en
Open access
closed
Locations
  • Plant Cell Reports (publishedVersion)
  • PubMed (publishedVersion)
  • Civil War Book Review (submittedVersion)
Funders
University of Cambridge
Topics
  • Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
Keywords
antimicrobial peptides, cecropin B, transgenic Arabidopsis, antifungal activity, antibacterial activity, plant pathogenic fungi, plant pathogenic bacteria, membrane permeabilization, endogenous promoter, plant disease resistance
MeSH terms
Fusarium, Plant Proteins, Promoter Regions, Genetic, Rhizoctonia, Arabidopsis, Plant Leaves, Insect Proteins, Antimicrobial Cationic Peptides, Plants, Genetically Modified, Pseudomonas syringae

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