Expression of the antimicrobial peptides in plants to control phytopathogenic bacteria and fungi
Abstract
Three antimicrobial peptides exhibiting in vitro antifungal activity were expressed in Arabidopsis to compare their in planta activity. Beta-Purothionin, cecropin B, and phor21 were expressed under an endogenous promoter with moderate-level activity and excreted extracellularly. Expression of beta-purothionin rendered the greatest antibacterial and antifungal resistance while cecropin B enhanced only antibacterial activity and phor21 did not improve antimicrobial resistance. The transgenic beta-purothionin arrested fungal growth on leaf surfaces and infection of stomata. Leaf extracts from plants producing beta-purothionin and cecropin B displayed membrane permeabilizing activity. The in planta antimicrobial activity of the tested peptides was consistent with previously reported in vitro experiments. The expression strategy allowed enhanced antifungal resistance without high-level transgene expression.
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Antimicrobial peptides could be expressed from a single heterologous gene, as has been shown in the nucleus and chloroplast of plants.
“As an alternative, antimicrobial peptides (AMPs) could be expressed from a single heterologous gene, as has been shown in the nucleus and chloroplast of plants [74,75].”
From Biofuels from algae: Challenges and potential (2010) · cites this paper as [75]
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Odintsova, T. I., Shiyan, A. N., & Slezina, M. P. (2025) · Russian Journal of Genetics · doi:10.1134/s102279542570111x · Cited by 1 · see citing works
Molecular Characterization and SNP Markers of the β-purothionin Gene in Einkorn Wheats
Jing-qiong, L. I., Zheng, Y., & Wei, Y. (2009) · Agricultural Sciences in China · doi:10.1016/s1671-2927(08)60309-0 · Cited by 1 · see citing works
Natural Antimicrobial Peptides: An Emerging Therapeutic Agent against Pathogens
Kumar, A., Yadav, S., Sankhwar, R., & Gupta, R. K. (2020) · International Journal of TROPICAL DISEASE & Health · doi:10.9734/ijtdh/2020/v41i1530354 · Cited by 1 · see citing works
Gujjar, R. S., Joshi, D., Srivast, S., KUMAR, R. R., Singh, S., Shiv, A., Goswami, S. K., Pathak, A. D., & Viswanathan, R. (2023) · Research Square · doi:10.21203/rs.3.rs-2558383/v1 · Cited by 1 · see citing works
Small cysteine-rich proteins from plants: a rish resource of antimicrobial agents
Bhave, M. R., & Methuku, D. R. (2011) · Swinburne figshare (Swinburne University of Technology)
STER1, a novel receptor-like kinase, functions in MAMP signalling in Arabidopsis
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Gujjar, R. S., KUMAR, R. R., Goswami, S. K., Joshi, D., Srivast, S., Singh, S., Shiv, A., Mall, A. K., Kumar, S., Kumar, A., et al. (2023) · Research Square · doi:10.21203/rs.3.rs-2720810/v1
Chromosome-Specific Expansion and Diversification of the Thionin Gene Family in BarleyPreprint
Fu, Y., Russell, J., Schreiber, M., & Bos, J. I. B. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.06.19.733385
Antimicrobial peptides in agriculture: Enhancing crop protection and food security
Kumar, S., Jaiwal, Y. K., Jaiwal, R., Chaudhary, D., Chhillar, A. K., Ahlawat, Y. K., & Jaiwal, P. K. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-36694-9.00015-1
- Authors
- Oard, S. V., & Enright, F. M.
- Affiliations
- Louisiana State University (US)
- Corresponding author
- Oard, S. V.
- Venue
- Plant Cell Reports
- Published
- 2006-02-03
- Volume
- 25
- Issue
- 6
- Pages
- 561-572
- ISSN
- 0721-7714 (ISSN-L)
- 1432-203X
- OpenAlex
- W2054434785
- Cited by
- 68
- References
- 44
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 2 · 2023: 4 · 2022: 3 · 2021: 2 · 2020: 3 · 2019: 3 · 2018: 4 · 2017: 2 · 2016: 1 · 2015: 2 · 2014: 5 · 2013: 3 · 2012: 8
- FWCI
- 3.26
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Plant Cell Reports (publishedVersion)
- PubMed (publishedVersion)
- Civil War Book Review (submittedVersion)
- Funders
- University of Cambridge
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
- Keywords
- antimicrobial peptides, cecropin B, transgenic Arabidopsis, antifungal activity, antibacterial activity, plant pathogenic fungi, plant pathogenic bacteria, membrane permeabilization, endogenous promoter, plant disease resistance
- MeSH terms
- Fusarium, Plant Proteins, Promoter Regions, Genetic, Rhizoctonia, Arabidopsis, Plant Leaves, Insect Proteins, Antimicrobial Cationic Peptides, Plants, Genetically Modified, Pseudomonas syringae
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