Inhibition of the gene expression for granule-bound starch synthase I by RNA interference in sweet potato plants
Abstract
Granule-bound starch synthase I (GBSSI) is one of the key enzymes catalyzing the formation of amylose, a linear alpha(1,4)D-glucan polymer, from ADP-glucose. Amylose-free transgenic sweet potato plants were produced by inhibiting sweet potato GBSSI gene expression through RNA interference. The gene construct consisting of an inverted repeat of the first exon separated by intron 1 of GBSSI driven by the CaMV 35S promoter was integrated into the sweet potato genome by Agrobacterium tumefaciens-mediated transformation. In over 70% of the regenerated transgenic plants, the expression of GBSSI was inactivated giving rise to storage roots containing amylopectin but not amylose. Electrophoresis analysis failed to detect the GBSSI protein, suggesting that gene silencing of the GBSSI gene had occurred. These results clearly demonstrate that amylose synthesis is completely inhibited in storage roots of sweet potato plants by the constitutive production of the double-stranded RNA of GBSSI fragments. We conclude that RNA interference is an effective method for inhibiting gene expression in the starch metabolic pathway.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Several genes were confirmed to influence starch content or composition in RNA interference studies.
“Several genes were confirmed to influence starch content or composition in RNA interference studies (Shimada et al., 2006; Kitahara et al., 2007; Otani et al., 2007).”
From Comparative Transcriptome Analysis Reveals Critical Function of Sucrose Metabolism Related-Enzymes in Starch Accumulation in the Storage Root of Sweet Potato (2017) · cites this paper as Otani et al., 2007
The starch content is reduced in the storage roots of transgenic sweet potato plants in which the expression of SBEII or GBSSI is suppressed by RNA interference.
“The starch content is reduced in the SRs of transgenic sweet potato plants in which the expression of SBEII (Shimada et al., 2006) or GBSSI (Otani et al., 2007) is suppressed by RNA interference.”
From Comparative Transcriptome Analysis Reveals Critical Function of Sucrose Metabolism Related-Enzymes in Starch Accumulation in the Storage Root of Sweet Potato (2017) · cites this paper as Otani et al., 2007
In sweet potato, amylose-free and high-amylose transgenic sweet potato plants have been produced by inhibiting the expression of genes encoding sweet potato GBSSI and SBEII through RNA interference, respectively.
“In sweet potato, amylose-free and high-amylose transgenic sweet potato plants have been produced by inhibiting the expression of genes encoding sweet potato GBSSI and SBEII through RNA interference, respectively (Shimada et al., 2006; Kitahara et al., 2007; Otani et al., 2007).”
From Comparative Transcriptome Analysis Reveals Critical Function of Sucrose Metabolism Related-Enzymes in Starch Accumulation in the Storage Root of Sweet Potato (2017) · cites this paper as Otani et al., 2007
Cites
A simple and efficient method for isolating RNA from pine trees
Chang, S., Puryear, J. D., & Cairney, J. (1993) · Plant Molecular Biology Reporter · doi:10.1007/bf02670468 · Cited by 3,559 · see citing works
Organic Growth Factor Requirements of Tobacco Tissue Cultures
Linsmaier-Bednar, E. M., & Skoog, F. K. (1965) · Physiologia Plantarum · doi:10.1111/j.1399-3054.1965.tb06874.x · Cited by 3,491 · see citing works
Visser, R. G. F., Somhorst, I., Kuipers, G. J., Ruys, N. J., Feenstra, W. J., & Jacobsen, E. (1991) · Molecular and General Genetics MGG · doi:10.1007/bf00269861 · Cited by 289 · see citing works
hpRNA-Mediated Targeting of the Arabidopsis FAD2 Gene Gives Highly Efficient and Stable Silencing
Stoutjesdijk, P. A., Singh, S. P., Liu, Q., Hurlstone, C., Waterhouse, P. M., & Green, A. G. (2002) · PLANT PHYSIOLOGY · doi:10.1104/pp.006353 · Cited by 175 · see citing works
Kuipers, A., Jacobsen, E., & Visser, R. G. F. (1994) · The Plant Cell · doi:10.1105/tpc.6.1.43 · Cited by 166 · see citing works
Ogita, S., Uefuji, H., Morimoto, M., & Sano, H. (2004) · Plant Molecular Biology · doi:10.1007/s11103-004-0393-x · Cited by 160 · see citing works
Physicochemical properties of amylose-free starch from transgenic sweet potato
Noda, T. (2002) · Carbohydrate Polymers · doi:10.1016/s0144-8617(01)00343-5 · Cited by 87 · see citing works
Kim, S.-H. (2002) · Plant and Cell Physiology · doi:10.1093/pcp/pcf043 · Cited by 84 · see citing works
Self-citationHeeres, P., Schippers, M. G. M., Jacobsen, E., & Visser, R. G. F. (2002) · Euphytica · doi:10.1023/a:1015689112703 · Cited by 78 · see citing works
Shimada, T., Otani, M., Hamada, T., & Kim, S.-H. (2006) · Plant Biotechnology · doi:10.5511/plantbiotechnology.23.85 · Cited by 77 · see citing works
Self-citationShimada, H., Tada, Y., Kawasaki, T., & Fujimura, T. (1993) · Theoretical and Applied Genetics · doi:10.1007/bf00222654 · Cited by 74 · see citing works
Kimura, T., Otani, M., Noda, T., Ideta, O., Shimada, T., & Saito, A. (2001) · Plant Cell Reports · doi:10.1007/s002990100376 · Cited by 64 · see citing works
Self-citationOtani, M., Shimada, T., Kimura, T., & Saito, A. (1998) · Plant Biotechnology · doi:10.5511/plantbiotechnology.15.11 · Cited by 58 · see citing works
Self-citationKuipers, A. G. J., Soppe, W. J. J., Jacobsen, E., & Visser, R. G. F. (1995) · Molecular and General Genetics MGG · doi:10.1007/bf00290722 · Cited by 46 · see citing works
Kuipers, A. G. J., Soppe, W. J. J., Jacobsen, E., & Visser, R. G. F. (1994) · Plant Molecular Biology · doi:10.1007/bf00019490 · Cited by 45 · see citing works
Heilersig, H. J. B., Loonen, A. E. H. M., Bergervoet, M., Wolters, A. A., & Visser, R. G. F. (2006) · Plant Molecular Biology · doi:10.1007/s11103-005-5280-6 · Cited by 43 · see citing works
Otani, M., Wakita, Y., & Shimada, T. (2003) · Ikushugaku zasshi · doi:10.1270/jsbbs.53.145 · Cited by 37 · see citing works
Self-citationEfficient embryogenic callus formation in sweet potato(Ipomoea batatas(L.) Lam.).
Otani, M., & Shimada, T. (1996) · Ikushugaku zasshi · doi:10.1270/jsbbs1951.46.257 · Cited by 27 · see citing works
Self-citationHamada, T., Kodama, H., Nishimura, M., & Iba, K. (1996) · Transgenic Research · doi:10.1007/bf01969429 · Cited by 24 · see citing works
Self-citationKimura, T., Ideta, O., & SAITO, A. (2000) · Plant Biotechnology · doi:10.5511/plantbiotechnology.17.247 · Cited by 21 · see citing works
Induction of citric acid overproduction in Aspergillus niger on beet molasses
Podgórski, W., & Leśniak, W. (2000) · Progress in biotechnology · doi:10.1016/s0921-0423(00)80075-1 · Cited by 3 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
RNA interference: concept to reality in crop improvement
Saurabh, S., Vidyarthi, A. S., & Prasad, D. (2014) · Planta · doi:10.1007/s00425-013-2019-5 · Cited by 252 · see citing works
Seung, D., Soyk, S., Coiro, M., Maier, B. A., Eicke, S., & Zeeman, S. C. (2015) · PLoS Biology · doi:10.1371/journal.pbio.1002080 · Cited by 199 · see citing works
Impact of amylose content on starch physicochemical properties in transgenic sweet potato
Zhou, W., Yang, J., Hong, Y., Liu, G., Zheng, J., Gu, Z., & Zhang, P. (2015) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2014.11.003 · Cited by 170 · see citing works
Wang, H., Wu, Y., Zhang, Y., Yang, J. M., Fan, W., Zhang, H., Zhao, S., Yuan, L., & Zhang, P. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20194702 · Cited by 156 · see citing works
Improvement for agronomically important traits by gene engineering in sweetpotato
Liu, Q. (2017) · Ikushugaku zasshi · doi:10.1270/jsbbs.16126 · Cited by 112 · see citing works
Exploring and exploiting genetics and genomics for sweetpotato improvement: Status and perspectives
Yan, M., Nie, H., Wang, Y., Wang, X., Jarret, R. L., Zhao, J., Wang, H., & Yang, J. M. (2022) · Plant Communications · doi:10.1016/j.xplc.2022.100332 · Cited by 104 · see citing works
Lai, Y. C., Wang, S. Y., Gao, H. Y., Nguyen, K. M., Nguyen, C. H., Shih, M. C., & Lin, K. H. (2016) · Food Chemistry · doi:10.1016/j.foodchem.2015.12.053 · Cited by 100 · see citing works
Zhang, K., Wu, Z., Tang, D., Luo, K., Lu, H., Liu, Y., Dong, J., Wang, X., Lv, C., Wang, J., et al. (2017) · Frontiers in Plant Science · doi:10.3389/fpls.2017.00914 · Cited by 88 · see citing works
Zhao, S., Dufour, D., Sánchez, T. S., Ceballos, H., & Zhang, P. (2011) · Biotechnology and Bioengineering · doi:10.1002/bit.23120 · Cited by 84 · see citing works
Bharathi, J. K., Anandan, R., Benjamin, L. K., Muneer, S., & Prakash, M. (2022) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2022.11.035 · Cited by 75 · see citing works
Exogenous sucrose utilization and starch biosynthesis among sweetpotato cultivars
Ahn, Y. O., Kim, S. H., Kim, C. Y., Lee, J.-S., Kwak, S., & Haeng-Soon, L. E. E. (2009) · Carbohydrate Research · doi:10.1016/j.carres.2009.08.025 · Cited by 65 · see citing works
Self-citationMarsolais, F., Pająk, A., Yin, F., Taylor, M., Gabriel, M. A., Merino, D. M., Vanessa, M., Kameka, A., Vijayan, P., Pham, H., et al. (2010) · Journal of Proteomics · doi:10.1016/j.jprot.2010.03.013 · Cited by 63 · see citing works
Lyu, R., Ahmed, S., Fan, W., Yang, J. M., Wu, X., Zhou, W., Zhang, P., Yuan, L., & Wang, H. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22179533 · Cited by 67 · see citing works
Carbohydrate components in sweetpotato storage roots: their diversities and genetic improvement
Kitahara, K., Nakamura, Y., Otani, M., Hamada, T., Nakayachi, O., & Takahata, Y. (2017) · Ikushugaku zasshi · doi:10.1270/jsbbs.16135 · Cited by 62 · see citing works
Self-citationTakahata, Y., Tanaka, M., Otani, M., Katayama, ., Kitahara, K., Nakayachi, O., Nakayama, H., & Yoshinaga, M. (2010) · Plant Cell Reports · doi:10.1007/s00299-010-0842-8 · Cited by 60 · see citing works
Self-citationRen, Z., He, S., Zhao, N., Zhai, H., & Liu, Q. (2018) · Plant Biotechnology Journal · doi:10.1111/pbi.12944 · Cited by 61 · see citing works
Yang, J. M., Bi, H., Fan, W., Zhang, M., Wang, H., & Zhang, P. (2011) · Plant Science · doi:10.1016/j.plantsci.2011.01.005 · Cited by 55 · see citing works
Molecular Regulation of Storage Root Formation and Development in Sweet Potato
Ravi, V., Chakrabarti, S. K., Makeshkumar, T., & Saravanan, R. (2014) · Horticultural Reviews: Volume 42 · doi:10.1002/9781118916827.ch03 · Cited by 53 · see citing works
Gao, M., Zhang, S., Luo, C., He, X., Wei, S., Jiang, W. J., He, F., Lin, Z., Yan, M., & Dong, W. (2018) · Gene · doi:10.1016/j.gene.2018.01.075 · Cited by 45 · see citing works
Potato: from functional genomics to genetic improvement
Li, Q., Huang, X., Su, X., Zhu, G., Zheng, L., Lin, J., Wang, J., & Xue, H. (2024) · Molecular Horticulture · doi:10.1186/s43897-024-00105-3 · Cited by 46 · see citing works
Miao, H., Sun, P., Liu, W., Xu, B., & Jin, Z. (2014) · PLoS ONE · doi:10.1371/journal.pone.0088077 · Cited by 38 · see citing works
Reinvention of starch for oral drug delivery system design
Ab’lah, N. N., Yusuf, C. Y. L., Rojsitthisak, P., & Wong, T. W. (2023) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2023.124506 · Cited by 27 · see citing works
Wang, L., Liu, L., Zhao, J., Li, C., Wu, H., Zhao, H., & Wu, Q. (2023) · Plant Science · doi:10.1016/j.plantsci.2023.111843 · Cited by 27 · see citing works
Lee, B.-S., Koo, K. M., Ryu, J., Hong, M. J., Kim, S. H., Kwon, S., Kim, J., Choi, J., & Ahn, J. (2020) · Algal Research · doi:10.1016/j.algal.2020.101825 · Cited by 24 · see citing works
Self-citationCRISPR/Cas mediated genome editing in potato: Past achievements and future directions
Tuncel, A., & Qi, Y. (2022) · Plant Science · doi:10.1016/j.plantsci.2022.111474 · Cited by 23 · see citing works
Three strategies of transgenic manipulation for crop improvement
Yu, H., Yang, Q., Fu, F., & Li, W. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.948518 · Cited by 19 · see citing works
Dong, W., He, F., HuiPing, J., Liu, L., & Qiu, Z. (2021) · Frontiers in Genetics · doi:10.3389/fgene.2021.771081 · Cited by 18 · see citing works
Occurrence of internal browning in tuberous roots of sweetpotato and its related starch biosynthesis
Fukuoka, N., Miyata, M., Hamada, T., & Takeshita, E. (2018) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2018.12.007 · Cited by 17 · see citing works
Self-citationNakkanong, K., Yang, J. H., & Zhang, M. F. (2012) · Scientia Horticulturae · doi:10.1016/j.scienta.2011.12.020 · Cited by 17 · see citing works
Wirojsirasak, W., Kalapanulak, S., & Saithong, T. (2019) · PLoS ONE · doi:10.1371/journal.pone.0210481 · Cited by 12 · see citing works
Modification of Starch Composition Using RNAi Targeting Soluble Starch Synthase I in Japonica Rice
Lee, H., Jee, M.-G., Kim, J., Nogoy, F. M., o, M. C. N., Yu, D.-A., Kim, M. S., Sun, M., Kang, K.-K., Nou, I., et al. (2014) · Plant Breeding and Biotechnology · doi:10.9787/pbb.2014.2.3.301 · Cited by 12 · see citing works
Lesponne, I., Naar, J. P., Planchon, S., Serchi, T., & Montano, M. (2018) · Veterinary Sciences · doi:10.3390/vetsci5030063 · Cited by 11 · see citing works
Bansal, A., Kumari, V., Taneja, D., Sayal, R., & Das, N. (2011) · Plant Cell Tissue and Organ Culture (PCTOC) · doi:10.1007/s11240-011-0090-9 · Cited by 11 · see citing works
Zhang, K., Luo, K., Li, S., Peng, D., Tang, D., Lu, H., Zhao, Y., Lv, C., & Wang, J. (2020) · Agronomy · doi:10.3390/agronomy10050627 · Cited by 12 · see citing works
Haque, E., Shirasawa, K., Suematsu, K., Tabuchi, H., Isobe, S., & Tanaka, M. (2023) · Frontiers in Plant Science · doi:10.3389/fpls.2023.1181909 · Cited by 13 · see citing works
RNA interference: a promising technique for the improvement of traditional crops
Katoch, R., & Thakur, N. (2012) · International Journal of Food Sciences and Nutrition · doi:10.3109/09637486.2012.713918 · Cited by 10 · see citing works
Unveiling the Benefits of Resistant Starch in Legumes: Overview of Current and Future Prospective
Ghare, M., Vishwakarma, K., Tripathi, S., DIXIT, G. P., & Soren, K. R. (2024) · Starch - Stärke · doi:10.1002/star.202300212 · Cited by 9 · see citing works
Expression profiles of genes involved in starch synthesis in non-waxy and waxy wheat
Cao, Y. N., Hu, W. G., & Wang, C. S. (2012) · Russian Journal of Plant Physiology · doi:10.1134/s1021443712050056 · Cited by 9 · see citing works
Zhang, E.-J., Shen, W., Jiang, W., Li, W., Wan, X.-P., Yu, X., & Xiong, F. (2023) · PeerJ · doi:10.7717/peerj.15400 · Cited by 8 · see citing works
Transgenic Research in Tuber and Root Crops
Si, H., Zhang, N., Tang, X., Yang, J., Wen, Y., Wang, L., & Zhou, X. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-810439-2.00011-8 · Cited by 8 · see citing works
The use of RNA interference for the metabolic engineering of plants (Review)
Борисовна, Р. Е., Alekseeva, V. V., & Buryanov, Y. I. (2010) · Russian Journal of Bioorganic Chemistry · doi:10.1134/s1068162010020020 · Cited by 8 · see citing works
Kou, M., Su, Z.-X., Zhang, Y., & Li, Q. (2020) · Plant Biotechnology Reports · doi:10.1007/s11816-020-00647-0 · Cited by 7 · see citing works
Kumari, N., Kumar, A., Sharma, S., Thakur, P., Chadha, S., & Dhiman, A. (2024) · Journal of Plant Biochemistry and Biotechnology · doi:10.1007/s13562-024-00878-0 · Cited by 6 · see citing works
Seo, S.-G., Bea, S.-H., Jun, B.-K., Kim, S. T., Kwon, S., & Kim, S. (2015) · Genes & Genomics · doi:10.1007/s13258-015-0289-y · Cited by 6 · see citing works
Self-citationImpacts of cultivation practices on internal browning in sweetpotato
Fukuoka, N., Miyata, M., Hamada, T., Takeshita, E., & Masuda, D. (2019) · The Journal of Horticultural Science and Biotechnology · doi:10.1080/14620316.2019.1625289 · Cited by 4 · see citing works
Self-citationSun, Z., Li, Z., Lin, X., Hu, Z., Jiang, M., Tang, B., Zhao, Z., Xing, M., Yang, X., & Zhu, H. (2024) · Genes · doi:10.3390/genes15040400 · Cited by 3 · see citing works
Biofortified sweet potato—an ideal source of mitigating hidden hunger
Hamda, Ahmad, M. Q., Saleem, A., Yan, H., & Li, Q. (2024) · Elsevier eBooks · doi:10.1016/b978-0-323-91735-3.00013-3 · Cited by 3 · see citing works
Plant RNAi and Crop Improvement
Isshiki, M., & Kodama, H. (2009) · Springer eBooks · doi:10.1007/978-90-481-2967-6_28 · Cited by 2 · see citing works
Kim, M., Ahn, Y.-O., Kim, Y., Kim, C.-Y., Lee, J.-J., Jeong, J.-C., Haeng-Soon, L. E. E., Mok, I.-G., & Kwak, S. (2009) · Journal of Plant Biotechnology · doi:10.5010/jpb.2009.36.3.197 · Cited by 2 · see citing works
Current status on metabolic engineering of starch in sweetpotato
Ahn, Y. O., Yang, K.-S., Kim, S., Kwak, S., & Haeng-Soon, L. E. E. (2009) · Journal of Plant Biotechnology · doi:10.5010/jpb.2009.36.3.207 · Cited by 2 · see citing works
Self-citation(2011) · Journal of Applied Glycoscience · doi:10.5458/jag.jag.jag-2011_010 · Cited by 2 · see citing works
Quality evaluation of sweet potato products
Nakamura, Y. (2019) · Elsevier eBooks · doi:10.1016/b978-0-12-813637-9.00013-2 · Cited by 2 · see citing works
Genetic Modification of Tropical Root and Tuber Crops: Prospects and Perspectives
Parvathy, S. T., & Sheela, M. N. (2024) · Springer eBooks · doi:10.1007/978-981-97-3119-0_6 · Cited by 2 · see citing works
MORPHOLOGICAL AND THERMAL PROPERTIES OF MAIZE STARCH
Popescu, E. C., Stoica, A., Bărăscu, E., & Iordan, M. (2010) · DOAJ (DOAJ: Directory of Open Access Journals) · Cited by 1 · see citing works
Peng, M., Liao, Z., Yue, Z., Yang, C., Zeng, L., Chen, M., Ming, X., & Lan, X. (2011) · Journal of Medicinal Plants Research · doi:10.5897/jmpr.9000577 · Cited by 1 · see citing works
Characterization of Genes and Promoters, Transformation and Transgenic Development in Sweet Potato
Mohan, C., & Nair, A. G. H. (2012) · Cited by 1 · see citing works
Carbohydrate Components Associated with Sweetness of Cooked Storage Roots of Sweet Potato Cultivars
Nakamura, Y. (2020) · NIPPON SHOKUHIN KOGYO GAKKAISHI · doi:10.3136/nskkk.67.305 · Cited by 1 · see citing works
Wally, O. S. D., Jayaraj, J., & Punja, Z. K. (2011) · Taylor & Francis eBooks · doi:10.1201/b10952-3 · Cited by 1 · see citing works
Kim, H. S., Yoon, U.-H., Lee, C.-J., Kim, S.-E., Ji, C. Y., & Kwak, S. (2018) · Journal of Plant Biotechnology · doi:10.5010/jpb.2018.45.3.196 · Cited by 1 · see citing works
Self-citationCloning of Granule-Bound Starch Synthase Gene and Construction of Its RNAi Vector in Potato Tuber
Liu, Y., Li, W. A. N. G., Yang, H., Yu, B., Li, Y., Zhang, J., & Wang, L. (2013) · ACTA AGRONOMICA SINICA · doi:10.3724/sp.j.1006.2012.01187 · Cited by 1 · see citing works
Virulence of Fusarium spp. Causing Wilt Disease of Guava with Special Reference to RNAi
Jain, P., Manimaran, A., Turner, K., O’Donovan, A., & Tuohy, M. G. (2011) · CRC Press eBooks · doi:10.1201/b10882-19
RNA Interference and Wheat Functional Genomics
Sharma, P. K., & Rajender, S. (2011) · CRC Press eBooks · doi:10.1201/b10882-16
Phytomicrobiome: ecology, physiology, and emerging trends in microbial applications
Mathur, A., Kohli, D., Badhwar, U., Singh, R., & Khan, M. S. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-32932-6.00016-3
Transgenic modified natural starch
Zhang, P., Wang, Y., Qiao, Y., Osei, C. Y., Guo, S., & Ma, Q. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-36324-5.00003-8
- Authors
- Otani, M., Hamada, T., Katayama, K., Kitahara, K., Kim, S.-H., Takahata, Y., Suganuma, T., & Shimada, T.
- Affiliations
- Ishikawa Prefectural University (JP)
- Kyushu Okinawa Agricultural Research Center (JP)
- Kagoshima University (JP)
- University of Seoul (KR)
- Corresponding author
- Otani, Motoyasu
- ORCID
- Kim, Sun-Hyung
- Venue
- Plant Cell Reports
- Published
- 2007-07-12
- Volume
- 26
- Issue
- 10
- Pages
- 1801-1807
- ISSN
- 0721-7714 (ISSN-L)
- 1432-203X
- OpenAlex
- W2021140426
- Cited by
- 74
- References
- 21
- Citations by year
- 2026: 2 · 2024: 6 · 2023: 13 · 2022: 4 · 2021: 2 · 2020: 4 · 2019: 4 · 2018: 6 · 2017: 3 · 2015: 3 · 2014: 6 · 2013: 1 · 2012: 4
- FWCI
- 3.23
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Plant Cell Reports (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Keywords
- granule-bound starch synthase I, RNA interference, gene silencing, Ipomoea batatas, amylose synthesis, amylopectin, starch metabolism, transgenic plants, Agrobacterium-mediated transformation, CaMV 35S promoter, double-stranded RNA
- MeSH terms
- Amylose, Starch Synthase, Transformation, Genetic, Gene Expression Regulation, Plant, Ipomoea batatas, Plants, Genetically Modified, RNA Interference, Plant Tubers
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review