Cited by 74Plant Molecular Biology ResearchPlant nutrient uptake and metabolismPlant tissue culture and regeneration

Inhibition of the gene expression for granule-bound starch synthase I by RNA interference in sweet potato plants

Otani, Motoyasu, Hamada, Tatsuro, Katayama, Kenji, Kitahara, Kakefumi, Kim, Sun-Hyung, Takahata, Yasuhiro, Suganuma, Toshihiko, Shimada, Takiko·2007·Plant Cell Reports

Abstract

Granule-bound starch synthase I (GBSSI) is one of the key enzymes catalyzing the formation of amylose, a linear alpha(1,4)D-glucan polymer, from ADP-glucose. Amylose-free transgenic sweet potato plants were produced by inhibiting sweet potato GBSSI gene expression through RNA interference. The gene construct consisting of an inverted repeat of the first exon separated by intron 1 of GBSSI driven by the CaMV 35S promoter was integrated into the sweet potato genome by Agrobacterium tumefaciens-mediated transformation. In over 70% of the regenerated transgenic plants, the expression of GBSSI was inactivated giving rise to storage roots containing amylopectin but not amylose. Electrophoresis analysis failed to detect the GBSSI protein, suggesting that gene silencing of the GBSSI gene had occurred. These results clearly demonstrate that amylose synthesis is completely inhibited in storage roots of sweet potato plants by the constitutive production of the double-stranded RNA of GBSSI fragments. We conclude that RNA interference is an effective method for inhibiting gene expression in the starch metabolic pathway.

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Crossref · 10.1007/s00299-007-0396-6

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Authors
Otani, M., Hamada, T., Katayama, K., Kitahara, K., Kim, S.-H., Takahata, Y., Suganuma, T., & Shimada, T.
Affiliations
  • Ishikawa Prefectural University (JP)
  • Kyushu Okinawa Agricultural Research Center (JP)
  • Kagoshima University (JP)
  • University of Seoul (KR)
Corresponding author
Otani, Motoyasu
Venue
Plant Cell Reports
Published
2007-07-12
Volume
26
Issue
10
Pages
1801-1807
ISSN
  • 0721-7714 (ISSN-L)
  • 1432-203X
OpenAlex
W2021140426
Cited by
74
References
21
Citations by year
2026: 2 · 2024: 6 · 2023: 13 · 2022: 4 · 2021: 2 · 2020: 4 · 2019: 4 · 2018: 6 · 2017: 3 · 2015: 3 · 2014: 6 · 2013: 1 · 2012: 4
FWCI
3.23
Type
journal article
Language
en
Open access
closed
Locations
  • Plant Cell Reports (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
Keywords
granule-bound starch synthase I, RNA interference, gene silencing, Ipomoea batatas, amylose synthesis, amylopectin, starch metabolism, transgenic plants, Agrobacterium-mediated transformation, CaMV 35S promoter, double-stranded RNA
MeSH terms
Amylose, Starch Synthase, Transformation, Genetic, Gene Expression Regulation, Plant, Ipomoea batatas, Plants, Genetically Modified, RNA Interference, Plant Tubers

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