Cited by 126CRISPR and Genetic EngineeringPlant Genetic and Mutation StudiesGenetically Modified Organisms Research

The DOI matches its Crossref record; 79 of the 79 references it cites were found in a registry or an index.

The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato

Open access

Veillet, Florian, Chauvin, Laura, Kermarrec, Marie-Paule, Sevestre, François, Merrer, Mathilde, Terret, Zoé, Szydlowski, Nicolas, Devaux, Pierre, Gallois, Jean-Luc, Chauvin, Jean-Eric·2019·Plant Cell Reports

DOI 10.1007/s00299-019-02426-w

Published version of a preprint

Open this paper in CoChat — free account Open PDF

Abstract

Key message The StGBSSI gene was successfully and precisely edited in the tetraploid potato using gene and base-editing strategies, leading to plants with impaired amylose biosynthesis. Genome editing has recently become a method of choice for basic research and functional genomics, and holds great potential for molecular plant-breeding applications. The powerful CRISPR-Cas9 system that typically produces double-strand DNA breaks is mainly used to generate knockout mutants. Recently, the development of base editors has broadened the scope of genome editing, allowing precise and efficient nucleotide substitutions. In this study, we produced mutants in two cultivated elite cultivars of the tetraploid potato (Solanum tuberosum) using stable or transient expression of the CRISPR-Cas9 components to knock out the amylose-producing StGBSSI gene. We set up a rapid, highly sensitive and cost-effective screening strategy based on high-resolution melting analysis followed by direct Sanger sequencing and trace chromatogram analysis. Most mutations consisted of small indels, but unwanted insertions of plasmid DNA were also observed. We successfully created tetra-allelic mutants with impaired amylose biosynthesis, confirming the loss of function of the StGBSSI protein. The second main objective of this work was to demonstrate the proof of concept of CRISPR-Cas9 base editing in the tetraploid potato by targeting two loci encoding catalytic motifs of the StGBSSI enzyme. Using a cytidine base editor (CBE), we efficiently and precisely induced DNA substitutions in the KTGGL-encoding locus, leading to discrete variation in the amino acid sequence and generating a loss-of-function allele. The successful application of base editing in the tetraploid potato opens up new avenues for genome engineering in this species.

Integrity signals

Registry

Crossref · 10.1007/s00299-019-02426-w

Cites

79 of 79 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
Primer3—new capabilities and interfaces
Untergasser, A., Cutcutache, I., Kõressaar, T., Ye, J., Faircloth, B., Remm, M., & Rozen, S. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks596 · Cited by 9,165
Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis
Yoo, S.-D., Cho, Y.-H., & Sheen, J. (2007) · Nature Protocols · doi:10.1038/nprot.2007.199 · Cited by 5,104
Comparative Protein Structure Modeling Using MODELLER
Webb, B., & Šali, A. (2016) · Current Protocols in Bioinformatics · doi:10.1002/cpbi.3 · Cited by 4,404
Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage
Gaudelli, N. M., Komor, A. C., Rees, H. A., Packer, M. S., Badran, A. H., Bryson, D. I., & Liu, D. R. (2017) · Nature · doi:10.1038/nature24644 · Cited by 4,184
Statistical potential for assessment and prediction of protein structures
Shen, M., & Šali, A. (2006) · Protein Science · doi:10.1110/ps.062416606 · Cited by 2,294
Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR
Haeussler, M., Schönig, K., Eckert, H., Eschstruth, A., Mianné, J., Renaud, J.-B., Schneider-Maunoury, S., Shkumatava, A., Teboul, L., Kent, J., et al. (2016) · Genome biology · doi:10.1186/s13059-016-1012-2 · Cited by 1,756
Evolved Cas9 variants with broad PAM compatibility and high DNA specificity
Hu, J. H., Miller, S. M., Geurts, M. H., Tang, W., Chen, L., Sun, N., Zeina, C. M., Gao, X., Rees, H. A., Lin, Z., et al. (2018) · Nature · doi:10.1038/nature26155 · Cited by 1,552
Improving physical realism, stereochemistry, and side‐chain accuracy in homology modeling: Four approaches that performed well in CASP8
Krieger, E., Joo, K., Lee, J., Lee, J., Raman, S., Thompson, J., Tyka, M., Baker, D. A., & Karplus, K. J. (2009) · Proteins Structure Function and Bioinformatics · doi:10.1002/prot.22570 · Cited by 1,396
Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems
Nishida, K., Arazoe, T., Yachie, N., Banno, S., Kakimoto, M., Tabata, M., Mochizuki, M., Miyabe, A., Araki, M., Hara, K. Y., et al. (2016) · Science · doi:10.1126/science.aaf8729 · Cited by 1,349

Flagged first, then by citations

Show all 79 references

Sorted by citations

Precise plant genome editing using base editors and prime editors
Molla, K. A., Sretenovic, S., Bansal, K. C., & Qi, Y. (2021) · Nature Plants · doi:10.1038/s41477-021-00991-1 · Cited by 387
Reduced Enzymatic Browning in Potato Tubers by Specific Editing of a Polyphenol Oxidase Gene via Ribonucleoprotein Complexes Delivery of the CRISPR/Cas9 System
González, M. N., Massa, G. A., Andersson, M., Turesson, H., Olsson, N., Fält, A., Storani, L., Oneto, C. A. D., Hofvander, P., & Feingold, S. E. (2020) · Frontiers in Plant Science · doi:10.3389/fpls.2019.01649 · Cited by 285
Targeted genome-modification tools and their advanced applications in crop breeding
Li, B., Sun, C., Li, J., & Gao, C. (2024) · Nature Reviews Genetics · doi:10.1038/s41576-024-00720-2 · Cited by 269
Application of CRISPR/Cas9 in Crop Quality Improvement
Liu, Q., Yang, F., Zhang, J., Liu, H., Rahman, S., Islam, S., Ma, W., & She, M. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22084206 · Cited by 239
Development of Plant Prime-Editing Systems for Precise Genome Editing
Xu, R., Li, J., Liu, X., Shan, T., Qin, R., & Wei, P. (2020) · Plant Communications · doi:10.1016/j.xplc.2020.100043 · Cited by 209
Can gene editing reduce postharvest waste and loss of fruit, vegetables, and ornamentals?
Shipman, E. N., Yu, J., Zhou, J., Albornoz, K., & Beckles, D. M. (2020) · Horticulture Research · doi:10.1038/s41438-020-00428-4 · Cited by 141
Multiplex genome-editing technologies for revolutionizing plant biology and crop improvement
Abdelrahman, M., Wei, Z., Rohila, J. S., & Zhao, K. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.721203 · Cited by 125
CRISPR-Based Genome Editing for Nutrient Enrichment in Crops: A Promising Approach Toward Global Food Security
Kumar, D., Yadav, A., Ahmad, R., Dwivedi, U. N., & Yadav, K. (2022) · Frontiers in Genetics · doi:10.3389/fgene.2022.932859 · Cited by 139
Prime Editing in the model plant Physcomitrium patens and its potential in the tetraploid potato
Perroud, P., Guyon‐Debast, A., Veillet, F., Kermarrec, M.-P., Chauvin, L., Chauvin, J.-É., Gallois, J., & Nogué, F. (2021) · Plant Science · doi:10.1016/j.plantsci.2021.111162 · Cited by 130
Self-citation
Advances in Crop Breeding Through Precision Genome Editing
Nerkar, G., Devarumath, S., Purankar, M. V., Kumar, A., Valarmathi, R., Devarumath, R. M., & Appunu, C. (2022) · Frontiers in Genetics · doi:10.3389/fgene.2022.880195 · Cited by 124
Genome editing of polyploid crops: prospects, achievements and bottlenecks
Schaart, J. G., van de Wiel, C. C. M., & SMULDERS, M. J. M. (2021) · Transgenic Research · doi:10.1007/s11248-021-00251-0 · Cited by 113
Toward an understanding of potato starch structure, function, biosynthesis, and applications
Tong, C., Ma, Z., Chen, H., & Gao, H. (2023) · Food Frontiers · doi:10.1002/fft2.223 · Cited by 100
Mechanistic Concept of Physiological, Biochemical, and Molecular Responses of the Potato Crop to Heat and Drought Stress
Lal, M. K., Tiwari, R. K., Kumar, A., Dey, A., Kumar, R., Kumar, D., Jaiswal, A. K., Changan, S. S., Raigond, P., Dutt, S., et al. (2022) · Plants · doi:10.3390/plants11212857 · Cited by 107
Green Revolution to Gene Revolution: Technological Advances in Agriculture to Feed the World
Hamdan, M. F., Noor, S. N. M., Abd-Aziz, N., Pua, T., & Tan, B. C. (2022) · Plants · doi:10.3390/plants11101297 · Cited by 107
New Strategies to Overcome Present CRISPR/Cas9 Limitations in Apple and Pear: Efficient Dechimerization and Base Editing
Malabarba, J., Chevreau, E., Dousset, N., Veillet, F., Moizan, J., & Vergne, E. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms22010319 · Cited by 80
Self-citation
Evolution of plant mutagenesis tools: a shifting paradigm from random to targeted genome editing
Shelake, R. M., Pramanik, D., & Kim, J. (2019) · Plant Biotechnology Reports · doi:10.1007/s11816-019-00562-z · Cited by 79
Perspectives of CRISPR/Cas-mediated cis-engineering in horticulture: unlocking the neglected potential for crop improvement
Li, Q., Sapkota, M., & van der Knaap, E. (2020) · Horticulture Research · doi:10.1038/s41438-020-0258-8 · Cited by 73
Heritable gene editing using FT mobile guide RNAs and DNA viruses
Lei, J., Peihong, D., Li, Y., Zhang, W., Zhou, G., Liu, C., & Liu, X. (2021) · Plant Methods · doi:10.1186/s13007-021-00719-4 · Cited by 73
Editing sterol side chain reductase 2 gene ( StSSR2 ) via CRISPR/Cas9 reduces the total steroidal glycoalkaloids in potato
Zheng, Z., Ye, G., Zhou, Y., Pu, X., Su, W., & Wang, J. (2021) · All Life · doi:10.1080/26895293.2021.1925358 · Cited by 69
The caffeoyl-CoA O-methyltransferase gene SNP replacement in Russet Burbank potato variety enhances late blight resistance through cell wall reinforcement
Hegde, N., Joshi, S., Soni, N., & Kushalappa, A. C. (2020) · Plant Cell Reports · doi:10.1007/s00299-020-02629-6 · Cited by 65
Facilitating gene editing in potato: a Single-Nucleotide Polymorphism (SNP) map of the Solanum tuberosum L. cv. Desiree genome
Sevestre, F., Facon, M., Wattebled, F., & Szydlowski, N. (2020) · Scientific Reports · doi:10.1038/s41598-020-58985-6 · Cited by 64
Self-citation
Genome Editing Technology for Genetic Amelioration of Fruits and Vegetables for Alleviating Post-Harvest Loss
Kumari, C., Sharma, M., Kumar, V., Sharma, R., Kumar, V., Sharma, P., Kumar, P., & Irfan, M. (2022) · Bioengineering · doi:10.3390/bioengineering9040176 · Cited by 60
Expanding the CRISPR Toolbox in P. patens Using SpCas9-NG Variant and Application for Gene and Base Editing in Solanaceae Crops
Veillet, F., Perrot, L., Guyon‐Debast, A., Kermarrec, M.-P., Chauvin, L., Chauvin, J.-É., Gallois, J., Mazier, M., & Nogué, F. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21031024 · Cited by 59
Self-citation
CRISPR/Cas systems: opportunities and challenges for crop breeding
Biswas, S., Zhang, D., & Shi, J. (2021) · Plant Cell Reports · doi:10.1007/s00299-021-02708-2 · Cited by 58
CRISPR-induced indels and base editing using the Staphylococcus aureus Cas9 in potato
Veillet, F., Kermarrec, M.-P., Chauvin, L., Chauvin, J.-É., & Nogué, F. (2020) · PLoS ONE · doi:10.1371/journal.pone.0235942 · Cited by 55
Self-citation
Precision Breeding Made Real with CRISPR: Illustration through Genetic Resistance to Pathogens
Veillet, F., Durand, M., Kroj, T., Césari, S., & Gallois, J. (2020) · Plant Communications · doi:10.1016/j.xplc.2020.100102 · Cited by 60
Self-citation
Genome Engineering Technology for Durable Disease Resistance: Recent Progress and Future Outlooks for Sustainable Agriculture
Ali, Q., Yu, C., Hussain, A., Ali, M., Ahmar, S., Sohail, M. A., Riaz, M., Ashraf, M. F., Abdalmegeed, D., Wang, X., et al. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.860281 · Cited by 53
Potato: from functional genomics to genetic improvement
Li, Q., Huang, X., Su, X., Zhu, G., Zheng, L., Lin, J., Wang, J., & Xue, H. (2024) · Molecular Horticulture · doi:10.1186/s43897-024-00105-3 · Cited by 46
Comparative potato genome editing: Agrobacterium tumefaciens-mediated transformation and protoplasts transfection delivery of CRISPR/Cas9 components directed to StPPO2 gene
González, M. N., Massa, G. A., Andersson, M., Décima Oneto, C. A., Turesson, H., Storani, L., Olsson, N., Fält, A.-S., Hofvander, P., & Feingold, S. E. (2021) · Plant Cell, Tissue and Organ Culture (PCTOC) · doi:10.1007/s11240-020-02008-9 · Cited by 44
CRISPR/Cas Genome Editing in Potato: Current Status and Future Perspectives
Tiwari, J. K., Buckseth, T., Challam, C., Zinta, R., Bhatia, N., Dalamu, D., Naik, S., Poonia, A. K., Singh, R. K., Luthra, S. K., et al. (2022) · Frontiers in Genetics · doi:10.3389/fgene.2022.827808 · Cited by 44
Base Editors for Citrus Gene Editing
Huang, X., Wang, Y., & Wang, N. (2022) · Frontiers in Genome Editing · doi:10.3389/fgeed.2022.852867 · Cited by 44
Emerging applications of gene editing technologies for the development of climate-resilient crops
Chavhan, R. L., Jaybhaye, S. G., Hinge, V. R., Deshmukh, A. S., Shaikh, U. S., Jadhav, P. K., Kadam, U. S., & Hong, J. C. (2025) · Frontiers in Genome Editing · doi:10.3389/fgeed.2025.1524767 · Cited by 55
CRISPR/Cas9: Regulations and challenges for law enforcement to combat its dual-use
Mir, T. U. G., Wani, A. K., Akhtar, N., & Shukla, S. (2022) · Forensic Science International · doi:10.1016/j.forsciint.2022.111274 · Cited by 50
Multi-faceted CRISPR-Cas9 strategy to reduce plant based food loss and waste for sustainable bio-economy – A review
Palanivel, H., Abda, E. M., Shah, S., Prabhu, S. V., Jayakumar, M., Karmegam, N., Kim, W., & Govarthanan, M. (2023) · Journal of Environmental Management · doi:10.1016/j.jenvman.2023.117382 · Cited by 42
Recent advances and challenges in potato improvement using CRISPR/Cas genome editing
Chincinska, I., Miklaszewska, M., & Sołtys‐Kalina, D. (2022) · Planta · doi:10.1007/s00425-022-04054-3 · Cited by 41
Employing CRISPR/Cas Technology for the Improvement of Potato and Other Tuber Crops
TUSSIPKAN, D., & Manabayeva, S. A. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.747476 · Cited by 44
Research progress in gene editing technology
Huang, S., Yan, Y., Su, F., Huang, X., Xia, D., Jiang, X., Dong, Y., Lv, P., Chen, F., & Lv, Y. (2021) · Frontiers in Bioscience-Landmark · doi:10.52586/4997 · Cited by 38
State of the Art of Genetic Engineering in Potato: From the First Report to Its Future Potential
Nahirñak, V., Almasia, N. I., González, M. N., Massa, G. A., Oneto, C. A. D., Feingold, S. E., Hopp, H. E., & Vazquez-Rovere, C. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2021.768233 · Cited by 34
Genome editing in horticultural crops: augmenting trait development and stress resilience
Sohail, H., Noor, I., Hussain, H., Zhang, L., Xu, X., Chen, X., & Yang, X. (2025) · Horticultural Plant Journal · doi:10.1016/j.hpj.2025.09.001 · Cited by 34
The roles of starch branching enzymes and starch synthase in the biosynthesis of amylose in rice
Okpala, N. E., Aloryi, K. D., An, T., He, L., & Tang, X. (2022) · Journal of Cereal Science · doi:10.1016/j.jcs.2021.103393 · Cited by 29
Non‐GMO potato lines, synthesizing increased amylose and resistant starch, are mainly deficient in isoamylase debranching enzyme
Blennow, A., Skryhan, K., Tanackovic, V., Krunic, S. L., Shaik, S. S., Andersen, M. S., Kirk, H. G., & Nielsen, K. L. (2020) · Plant Biotechnology Journal · doi:10.1111/pbi.13367 · Cited by 28
CRISPR/Cas9-mediated fine-tuning of miRNA expression in tetraploid potato
Lukan, T., Veillet, F., Križnik, M., Coll, A., Povalej, T. M., Pogačar, K., Stare, K., Chauvin, L., Chauvin, J.-É., & Gruden, K. (2022) · Horticulture Research · doi:10.1093/hr/uhac147 · Cited by 28
Self-citation
Modern Plant Biotechnology: An Antidote against Global Food Insecurity
Animasaun, D. A., Adedibu, P. A., Shkryl, Y., Emmanuel, F. O., Tekutyeva, L., & Balabanova, L. (2023) · Agronomy · doi:10.3390/agronomy13082038 · Cited by 31
A blueprint for gene function analysis through Base Editing in the model plant Physcomitrium (Physcomitrella) patens
Guyon‐Debast, A., Alboresi, A., Terret, Z., Charlot, F., Berthier, F., Vendrell‐Mir, P., Casacuberta, J., Veillet, F., Morosinotto, T., Gallois, J., et al. (2021) · New Phytologist · doi:10.1111/nph.17171 · Cited by 29
Self-citation
Plant genome editing: CRISPR, base editing, prime editing, and beyond
Xie, Y., Haq, S. I. U., Jiang, X., Zheng, D., Feng, N., Wang, W., He, J., & Qiu, Q. (2022) · Grassland Research · doi:10.1002/glr2.12034 · Cited by 27
Potato trait development going fast-forward with genome editing
Hofvander, P., Andréasson, E., & Andersson, M. (2021) · Trends in Genetics · doi:10.1016/j.tig.2021.10.004 · Cited by 26
The Generic Risks and the Potential of SDN-1 Applications in Crop Plants
Kawall, K. (2021) · Plants · doi:10.3390/plants10112259 · Cited by 28
CRISPR/Cas mediated genome editing in potato: Past achievements and future directions
Tuncel, A., & Qi, Y. (2022) · Plant Science · doi:10.1016/j.plantsci.2022.111474 · Cited by 25
CRISPR/Cas genome editing system and its application in potato
Hou, X., Guo, X., Zhang, Y., & Zhang, Q. (2023) · Frontiers in Genetics · doi:10.3389/fgene.2023.1017388 · Cited by 23
CRISPR-Cas engineering in food science and sustainable agriculture: recent advancements and applications
Mohammadi, M. A., Maximiano, M. R., Hosseini, S. M., & Franco, O. L. (2023) · Bioprocess and Biosystems Engineering · doi:10.1007/s00449-022-02842-5 · Cited by 20
CRISPR/Cas9-mediated multiple guide RNA-targeted mutagenesis in the potato
Abeuova, L. S., Kali, B. R., TUSSIPKAN, D., Akhmetollayeva, A. S., Ramankulov, Y. M., & Manabayeva, S. A. (2023) · Transgenic Research · doi:10.1007/s11248-023-00356-8 · Cited by 22
Advanced Approaches for Biofortification
Kiran, K. (2020) · Springer eBooks · doi:10.1007/978-981-15-2874-3_2 · Cited by 20
Genome editing advancements in potato (Solanum tuberosum L.): operational challenges and solutions
Mali, S., Dutta, M., & Zinta, G. (2022) · Journal of Plant Biochemistry and Biotechnology · doi:10.1007/s13562-022-00812-2 · Cited by 18
CRISPR/Cas9: an advanced platform for root and tuber crops improvement
Divya, K., Thangaraj, M., & Radhika, N. K. (2024) · Frontiers in Genome Editing · doi:10.3389/fgeed.2023.1242510 · Cited by 20
Advancing vegetable genetics with gene editing: a pathway to food security and nutritional resilience in climate-shifted environments
Roychowdhury, R., Das, S. P., Das, S., Biswas, S., Patel, M. K., Kumar, A., Sarker, U., Pal, S., Das, R., Yogendra, K. N., et al. (2025) · Functional & Integrative Genomics · doi:10.1007/s10142-025-01533-0 · Cited by 20
Advances and Applications of Plant Base Editing Technologies
Peng, H. F., Li, J., Sun, K., Tang, H., Huang, W., Li, X., Wang, S., Ding, K., Han, Z., Li, Z., et al. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26199452 · Cited by 20
European genome editing regulations: threats to the European bioeconomy and unfit for purpose
Hjort, C., Cole, J. A., & Frébort, I. (2021) · EFB Bioeconomy Journal · doi:10.1016/j.bioeco.2021.100001 · Cited by 16
Modified Gene Editing Systems: Diverse Bioengineering Tools and Crop Improvement
Zhu, G., & Zhu, H. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.847169 · Cited by 16
Adoption of CRISPR-Cas for crop production: present status and future prospects
Akanmu, A. O., Asemoloye, M. D., Marchisio, M. A., & Babalola, O. O. (2024) · PeerJ · doi:10.7717/peerj.17402 · Cited by 14
Advanced Genome Editing Technologies: Potentials and Prospects in Improvement of Sugar crops
Amaresh, G., Nunavath, A., Appunu, C., Viswanathan, C., KUMAR, R. R., Gujjar, R. S., & Manimekalai, R. (2024) · Sugar Tech · doi:10.1007/s12355-024-01447-4 · Cited by 14
Comprehending the evolution of gene editing platforms for crop trait improvement
Dhakate, P., Sehgal, D., Vaishnavi, S., Chandra, A., Singh, A., Raina, S. N., & Rajpal, V. R. (2022) · Frontiers in Genetics · doi:10.3389/fgene.2022.876987 · Cited by 14
Tailoring crops with superior product quality through genome editing: an update
Ravikiran, K. T., Thribhuvan, R., Sheoran, S., Kumar, S., Kushwaha, A. K., Vineeth, T. V., & Saini, M. (2023) · Planta · doi:10.1007/s00425-023-04112-4 · Cited by 14
CRISPR/Cas technology in vegetable crops for improving biotic, abiotic stress and quality traits: Challenges and opportunities
Kushwaha, S. B., Nagesh, C. R., Lele, S. S., Viswanathan, C., Prashat, G. R., Goswami, S., Kumar, R. R., Kunchge, N. S., Gokhale, J. S., & Vinutha, T. (2025) · Scientia Horticulturae · doi:10.1016/j.scienta.2025.113957 · Cited by 11
Improvement of Crops Using the CRISPR/Cas System: New Target Genes
Ukhatova, Y., Erastenkova, M. V., Korshikova, E. S., Krylova, E. A., Mikhailova, A. S., Semilet, T. V., Tikhonova, N. G., Shvachko, N. A., & Хлесткина, Е. К. (2023) · Molecular Biology · doi:10.31857/s0026898423030151 · Cited by 11
Improvement of Crops Using the CRISPR/Cas System: New Target Genes
Ukhatova, Y. V., Erastenkova, M. V., Korshikova, E. S., Krylova, E. A., Mikhailova, A. S., Semilet, T. V., Tikhonova, N. G., Shvachko, N. A., & Хлесткина, Е. К. (2023) · Molecular Biology · doi:10.1134/s0026893323030135 · Cited by 11
Gene Editing in Potato Using CRISPR-Cas9 Technology
Chauvin, L., Sevestre, F., Lukan, T., Nogué, F., Gallois, J., Chauvin, J.-É., & Veillet, F. (2021) · Methods in molecular biology · doi:10.1007/978-1-0716-1609-3_16 · Cited by 10
Self-citation
CRISPR-Cas Gene Editing Technology in Potato
Сапахова, З., Канат, Р., Choi, K., Дауров, Д., Даурова, А., Жамбакин, К., & Шамекова, М. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26157496 · Cited by 9
Cytosine base editors optimized for genome editing in potato protoplasts
Westberg, I. N., Carlsen, F. M., Johansen, I. E., & Petersen, B. L. (2023) · Frontiers in Genome Editing · doi:10.3389/fgeed.2023.1247702 · Cited by 10
Mechanistic insights into granule-bound starch synthase I (GBSSI.L539P) allele in high amylose starch biosynthesis in wheat (Triticum aestivum L.)
Sharma, V., Jahan, K., Kumar, P., Puri, A., Sharma, V. K., Mishra, A., Bharatam, P. V., Sharma, D., Rishi, V., & Roy, J. K. (2022) · Functional & Integrative Genomics · doi:10.1007/s10142-022-00923-y · Cited by 9
Delivery of CRISPR/Cas9 Ribonucleoprotein Complex into Plant Apical Meristem Cells Leads to Large Deletions in an Editing Gene
Хромов, А. В., Makhotenko, A. V., Makarova, S. S., Suprunova, T. P., Kalinina, N. O., & Taliansky, M. E. (2020) · Russian Journal of Bioorganic Chemistry · doi:10.1134/s1068162020060138 · Cited by 8
CRISPR/Cas-Mediated Genome Editing for Improving Key Traits in Potato (Solanum tuberosum L.)
Sood, S., Dipta, B., Mangal, V., Thakur, A. K., Dutt, S., Sharma, N., Kumar, V. D., & Singh, B. (2024) · Journal of Plant Growth Regulation · doi:10.1007/s00344-024-11514-5 · Cited by 7
CRISPR/Cas9 Technology for Potato Functional Genomics and Breeding
González, M. N., Massa, G. A., Andersson, M., Storani, L., Olsson, N., Oneto, C. A. D., Hofvander, P., & Feingold, S. E. (2023) · Methods in molecular biology · doi:10.1007/978-1-0716-3131-7_21 · Cited by 7
CRISPR/Cas system for the traits enhancement in potato (Solanum tuberosum L.): present status and future prospectives
Kumari, N., Kumar, A., Sharma, S., Thakur, P., Chadha, S., & Dhiman, A. (2024) · Journal of Plant Biochemistry and Biotechnology · doi:10.1007/s13562-024-00878-0 · Cited by 6
Crop plants with improved culture and quality traits for food, feed and other uses
Nogué, F., Vergne, P., Chèvre, A., Chauvin, J.-É., Bouchabké‐Coussa, O., Déjardin, A., Chevreau, E., Oyant, L. H., Mazier, M., Barret, P., et al. (2019) · Transgenic Research · doi:10.1007/s11248-019-00135-4 · Cited by 6
Self-citation
From Lab to Field: CRISPRing Major Cultivated Solanaceae for Crop Improvement
Martina, F., Acquadro, A., & Moglia, A. (2026) · International Journal of Molecular Sciences · doi:10.3390/ijms27052238 · Cited by 5
Plant Mutagenesis Tools for Precision Breeding: Conventional CRISPR/Cas9 Tools and Beyond
Shelake, R. M., Pramanik, D., Waghunde, R. R., & Kim, J. (2023) · Springer eBooks · doi:10.1007/978-981-16-9720-3_10 · Cited by 6
Development and optimization of base editors and its application in crops
Jiang, C., Li, Y., Wang, R., Sun, X., Zhang, Y., & Zhang, Q. (2024) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2024.150942 · Cited by 7
Starch biosynthesis and crop bioengineering
Blennow, A., Hebelstrup, K. H., & Petersen, B. L. (2025) · Grain & Oil Science and Technology · doi:10.1016/j.gaost.2025.04.003 · Cited by 4
Emerging trends in the development of efficient CAS nucleases for meticulous gene editing in plants
Gujjar, R. S., Sherin, S., Batra, S., Mahto, A., Goswami, S. K., KUMAR, R. R., Tiwari, R. K., Singh, A., & Kumari, M. (2025) · Plant Science · doi:10.1016/j.plantsci.2025.112820 · Cited by 4
Rapid analysis of GBSS1 and Vinv genes expressed in potato tubers using microtubers produced in liquid culture medium
Wakasa, Y., Kasai, A., Yamazaki, M., Tabei, Y., Tsuyama, M., Igarashi, T., Okazaki, T., Yamamoto, K., Fujihara, H., Kanno, A., et al. (2020) · Plant Cell Reports · doi:10.1007/s00299-020-02572-6 · Cited by 4
Effect of a Radical Mutation in Plastidic Starch Phosphorylase PHO1a on Potato Growth and Cold Stress Response
Nezhdanova, A. V., Efremov, G. I., Slugina, M. A., Kamionskaya, A. M., Kochieva, E. Z., & Shchennikova, A. V. (2022) · Horticulturae · doi:10.3390/horticulturae8080730 · Cited by 4
The United States Potato Genebank Holding of cv. Desiree is a Somatic Mutant of cv. Urgenta
Amundson, K., Henry, I. M., & Comai, L. (2022) · American Journal of Potato Research · doi:10.1007/s12230-022-09892-1 · Cited by 4
Prospects for Genome Editing of Potato
Dev, S. S., Joseph, J., & Rosario, L. L. D. (2021) · IntechOpen eBooks · doi:10.5772/intechopen.99278 · Cited by 5
Nutrient Biofortification in Crop Plants by the CRISPR/Cas9 Technology: A Potential Approach for Sustainable Food Security
Sankhla, I. S., Kumar, A., & Singh, C. P. (2024) · Springer eBooks · doi:10.1007/978-981-99-8529-6_12 · Cited by 3
Genome Editing for Trait Improvement in Potato (Solanum tuberosum L.)
Mali, S., & Zinta, G. (2024) · Springer eBooks · doi:10.1007/978-981-97-3119-0_16 · Cited by 3
Recent Trends in Genome Editing Technologies for Agricultural Crop Improvement
Ahmad, H. M., Iqbal, M. S., Abdullah, M., El‐Tabakh, M. A. M., Oranab, S., Mudassar, M., Shimira, F., & Zahid, G. (2023) · Springer eBooks · doi:10.1007/978-3-031-15568-0_17 · Cited by 3
Transgenic and genome editing approaches for starch modification in potato- a review
Batta, S., Sharma, N., Gupta, R., Singh, B., & Thakur, A. K. (2026) · Plant Gene · doi:10.1016/j.plgene.2026.100585 · Cited by 2
Overcoming breeding barriers with genome editing in autopolyploid crops
Enciso-Rodríguez, F. E., Barrero, L. S., Garzón-Martínez, G. A., Kim, J. H., Kumam, Y., Pagliai, F. A., Jiang, T., Huo, H., & Muńoz, P. (2026) · BMC Plant Biology · doi:10.1186/s12870-026-08862-7 · Cited by 2
The transcription factor StC3H14 enhances cold tolerance through the CBF-dependent pathway in potato
Sun, S., Geng, H., Sina, Q., Fu, H., Wu, Y., Yang, R., Kong, W., Wang, Y., & Huang, B. (2026) · PLANT PHYSIOLOGY · doi:10.1093/plphys/kiag450 · Cited by 2
Biodiversidad, conservación y mejoramiento del cultivo de la papa (Solanum tuberosum) con énfasis en el grupo Chilotanum
Fernández, R., Lizana, C. N. R., & Behn, A. (2022) · Agro Sur · doi:10.4206/agrosur.2022.v50n2-01 · Cited by 2
Role of genetic engineering in improving potato production
Saeed, F., Dangol, S. D., Hashmi, M. H., Hossain, M. J., & Bakhsh, A. (2022) · Elsevier eBooks · doi:10.1016/b978-0-12-822925-5.00006-2 · Cited by 2
CRISPR-Cas and Its Applications in Food Production
Rai, N., Singh, N. A., Goyal, J., Upadhayay, P., & Jain, V. (2024) · Springer eBooks · doi:10.1007/978-981-99-8529-6_13 · Cited by 2
Commercialization of CRISPR-edited crops: Opportunities and challenges
Saxena, S., Sushmita, L. C., Babu, Y. M., Chavan, D. D., Venu, E., Mandal, S., Hurrah, I. M., Mohture, V. M., & Mishra, T. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-13229-2.00031-4 · Cited by 4
CRISPR: A Promising Toolbox for Better Crops
Sharma, N., Batta, S., Bhagta, S., Pathania, S., Dipta, B., Kumar, A., Singh, B., Siddappa, S., Sood, S., & Bhardwaj, V. (2024) · Springer eBooks · doi:10.1007/978-981-97-1223-6_2 · Cited by 2
Genome-wide association analysis and transgenic characterization for amylose content regulating gene in tuber of Dioscorea zingiberensis
Sun, S., Guan, B., Xing, Y., Li, X., Liu, L., Li, Y., Jia, L., Ye, S., Dossa, K., Li, Z., et al. (2024) · BMC Plant Biology · doi:10.1186/s12870-024-05122-4 · Cited by 1
Genome Editing in Potato Using CRISPR/Cas Technology: Applications and Challenges
Beniwal, D., Chauhan, S., & Dhillon, H. K. (2024) · Genetics. · doi:10.5772/intechopen.1003940 · Cited by 1
CRISPR/Cas: A precise and advanced tool for quality trait improvement in crops
Kumar, R., Singh, S., GANGWAR, L. K., Kumar, V., Ojre, A., Singh, A., Yadav, N., Kaur, N., Puri, P., Negi, R., et al. (2025) · Ecological Genetics and Genomics · doi:10.1016/j.egg.2025.100346 · Cited by 1
CRISPR/Cas system and AI tools for effective gene editing in vegetable crops: a systematic review
Kumar, M., Chaudhary, V., Chaudhary, V., Kumar, R., Sirohi, U., Singh, R., Singh, D., & Teotia, S. (2026) · Discover Applied Sciences · doi:10.1007/s42452-026-08305-w · Cited by 1
Advancements in genome editing tools and technologies for improvement of horticultural crops
Sivasankarreddy, K., Pandey, V., Pandey, S., Singh, S. K., & Patil, B. L. (2026) · Journal of Plant Biochemistry and Biotechnology · doi:10.1007/s13562-026-01079-7 · Cited by 1
Enzymatic Modification of Starch Using Recombinant Genes from Sorghum in Escherichia coli: Insights and Potential Applications
Kang, X., Gao, W., Cheng, Y., Cui, B., Qiao, X., & El‐Aty, A. M. A. (2023) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.3c07576 · Cited by 1

Authors: Veillet, F., Chauvin, L., Kermarrec, M.-P., Sevestre, F., Merrer, M., Terret, Z., Szydlowski, N., Devaux, P., Gallois, J.-L., & Chauvin, J.-E.
Affiliations: Institut Agro Rennes-Angers (FR)
Centre National de la Recherche Scientifique (FR)
Génétique et amélioration des fruits et légumes (FR)
Corresponding author: Veillet, Florian
ORCID: Veillet, Florian
Chauvin, Laura
Sevestre, François
Terret, Zoé
Szydlowski, Nicolas
Devaux, Pierre
Gallois, Jean-Luc
Venue: Plant Cell Reports
Publisher: Springer Science and Business Media LLC
Published: 2019-05-17
Volume: 38
Issue: 9
Pages: 1065-1080
ISSN: 0721-7714 (ISSN-L)
1432-203X
DOI: 10.1007/s00299-019-02426-w
OpenAlex: W2942972964
Cited by: 124
References: 79
Citations by year: 2026: 10 · 2025: 10 · 2024: 21 · 2023: 16 · 2022: 25 · 2021: 23 · 2020: 16 · 2019: 3
FWCI: 5.55
Type: journal article
Language: en
Open access: green
doi.org
Licence: cc-by-nc-nd
www.springer.com/tdm (tdm)
www.springer.com/tdm (vor)
Locations: Plant Cell Reports (publishedVersion)
bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
PubMed (publishedVersion)
HAL (submittedVersion)
LillOA (Université de Lille (University Of Lille)) (submittedVersion)
LillOA (Université de Lille (University Of Lille)) (submittedVersion, other-oa)
Funders: Agence Nationale de la Recherche (ANR-11-BTBR-0001_GENIUS)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
Keywords: tetraploid potato, potato, CRISPR/Cas9 base editing, amylose biosynthesis, starch synthase, loss-of-function mutants, high-resolution melting analysis, genome editing, molecular breeding
MeSH terms: Gene Editing, Alleles, Solanum tuberosum, Starch Synthase, Tetraploidy, CRISPR-Cas Systems

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks