Estimating the number of coding mutations in genotypic and phenotypic driven N-ethyl-N-nitrosourea (ENU) screens: revisited
Abstract
We recently described methods for estimating the number of N-ethyl-N-nitrosourea (ENU)-induced coding mutations in phenotypic and genotypic screens. In this article we revisit these methods, clarifying their application. In particular, we focus on the difference between unconditional and conditional probabilities. We also introduce a website to assist investigators in the application of these equations ( http://www.well.ox.ac.uk/enuMutRat ).
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The chance of a second point mutation occurring in a coding region in this linked interval is extremely small (p = 0.0008, based on a mutation rate of 1 in 1.82 Mb).
“The chance of a second point mutation occurring in a coding region in this linked interval is extremely small (p = 0.0008, based on a mutation rate of 1 in 1.82 Mb) [15-17].”
From A genome-wide screen for modifiers of transgene variegation identifies genes with critical roles in development (2008) · cites this paper as [15]
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- Authors
- Keays, D. A., Clark, T. G., Campbell, T. G., Broxholme, J., & Valdar, W.
- Affiliations
- Centre for Human Genetics (GB)
- Corresponding author
- Keays, David A.
- Venue
- Mammalian Genome
- Published
- 2007-02
- Volume
- 18
- Issue
- 2
- Pages
- 123-124
- ISSN
- 0938-8990 (ISSN-L)
- 1432-1777
- OpenAlex
- W2021794729
- Cited by
- 77
- References
- 43
- Citations by year
- 2019: 1 · 2018: 2 · 2017: 4 · 2016: 2 · 2015: 4 · 2014: 3 · 2013: 5 · 2012: 8
- FWCI
- 4.45
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Mammalian Genome (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genomics and Rare Diseases
- Keywords
- N-ethyl-N-nitrosourea, ENU mutagenesis screen, coding mutations, mutation rate estimation, conditional probability, phenotypic screening
- MeSH terms
- Ethylnitrosourea, Genetic Testing, Genotype, Mutation, Phenotype, Probability, Mutagenesis
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