Genome-wide, large-scale production of mutant mice by ENU mutagenesis
Open accessAbstract
In the post-genome era, the mouse will have a major role as a model system for functional genome analysis. This requires a large number of mutants similar to the collections available from other model organisms such as Drosophila melanogaster and Caenorhabditis elegans. Here we report on a systematic, genome-wide, mutagenesis screen in mice. As part of the German Human Genome Project, we have undertaken a large-scale ENU-mutagenesis screen for dominant mutations and a limited screen for recessive mutations. In screening over 14,000 mice for a large number of clinically relevant parameters, we recovered 182 mouse mutants for a variety of phenotypes. In addition, 247 variant mouse mutants are currently in genetic confirmation testing and will result in additional new mutant lines. This mutagenesis screen, along with the screen described in the accompanying paper, leads to a significant increase in the number of mouse models available to the scientific community. Our mutant lines are freely accessible to non-commercial users (for information, see http://www.gsf.de/ieg/groups/enu-mouse.html).
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Several mouse coat color mutants are ribosomal proteins, although they are not the ribosomal components enriched here.
“Pigment cell enrichment of ribosomal components is not surprising, considering several mouse coat color mutants are ribosomal proteins, although they are not the ribosomal components enriched here [50]–[53].”
From Gene Expression Analysis of Zebrafish Melanocytes, Iridophores, and Retinal Pigmented Epithelium Reveals Indicators of Biological Function and Developmental Origin (2013) · cites this paper as [53]
The indexed copy's bibliographic entry (section ‘1999 (7)’, no page) identifies the paper but provides no findings about coat color mutants or ribosomal proteins. Searches for those terms and for pigmentation returned only catalog material, so the claim cannot be checked against the available text. (LLM, conf=0.72)
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- Authors
- de Angelis, M. H., Flaswinkel, H., Fuchs, H., Rathkolb, B., Soewarto, D., Marschall, S., Heffner, S., Pargent, W., Wuensch, K., Jung, M., Reis, A., Richter, T., Alessandrini, F., Jakob, T., Fuchs, E., Kolb, H., Kremmer, E., Schaeble, K., Rollinski, B., Roscher, A., Peters, C., Meitinger, T., Strom, T., Steckler, T., Holsboer, F., Klopstock, T., Gekeler, F., Schindewolf, C., Jung, T., Avraham, K., Behrendt, H., Ring, J., Zimmer, A., Schughart, K., Pfeffer, K., Wolf, E., & Balling, R.
- Affiliations
- Technical University of Munich (DE)
- Helmholtz Munich (DE)
- Ludwig-Maximilians-Universität München (DE)
- Max Delbrück Center (DE)
- München Klinik Harlaching (DE)
- University of Freiburg (DE)
- Max Planck Institute of Psychiatry (DE)
- Max Planck Society (DE)
- Federal Office for Radiation Protection (DE)
- Tel Aviv University (IL)
- National Institute of Mental Health (US)
- Transgene (France) (FR)
- ORCID
- Venue
- Nature Genetics
- Published
- 2000-08
- Volume
- 25
- Issue
- 4
- Pages
- 444-447
- ISSN
- 1061-4036 (ISSN-L)
- 1546-1718
- OpenAlex
- W2121157101
- Cited by
- 664
- References
- 0
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 11 · 2023: 6 · 2022: 6 · 2021: 10 · 2020: 7 · 2019: 9 · 2018: 9 · 2017: 17 · 2016: 10 · 2015: 17 · 2014: 20 · 2013: 23 · 2012: 38
- FWCI
- 78.79
- Type
- journal article
- Language
- en
- Open access
- green
- mediatum.ub.tum.de
- Licence
- other-oa
- www.springer.com/tdm (tdm)
- Locations
- Nature Genetics (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- FreiDok plus (Universitätsbibliothek Freiburg) (submittedVersion)
- mediaTUM (Technical University of Munich) (submittedVersion, other-oa)
- Site cant be reached (submittedVersion)
- Open Repository and Bibliography (University of Luxembourg) (submittedVersion, cc-by-nc-sa)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Keywords
- ENU mutagenesis, mouse mutants, dominant mutations, recessive mutations, mouse model, functional genomics
- MeSH terms
- Animals, Crosses, Genetic, Ethylnitrosourea, Female, Forelimb, Immunoglobulin E, Immunoglobulin G, Immunoglobulin M, Immunity, Male, Mice, Inbred C3H, Mice, Inbred C57BL, Mutagens, Mutation, Phenotype, Cryopreservation, Mutagenesis, Genome, Mice
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