Ethanol and acetone stimulate the proliferation of HaCaT keratinocytes
Abstract
Alcohol has been reported to be a risk factor in psoriasis mainly based on the observation that there is a higher prevalence of alcohol abuse in individuals with psoriasis. The mechanism by which alcohol affects this disease is still elusive. So far there are no reports describing the effects of metabolites relevant to alcohol metabolism on the growth of human keratinocytes. In the present study we examined the effects of ethanol and acetone, which exceeds its normal endogenous level in the blood of heavy drinkers, on the proliferation of HaCaT keratinocytes. HaCaT cells were incubated for 30 min in the presence of various concentrations of ethanol (2.14 m M-1.71 M) and acetone (1.7 mM-1.36 M). The numbers of viable and proliferating cells were determined at different times after ethanol and acetone treatment. The effects of ethanol and acetone on the mRNA levels of genes characteristic for proliferating keratinocytes such as alpha5 integrin, keratinocyte growth factor receptor and cyclin D1 were studied by reverse-transcriptase polymerase chain reaction. Both ethanol and acetone induced proliferation of HaCaT cells. The maximum increase in the number of viable cells and the maximum proliferative response was observed with 4.28 m M ethanol and 13.6 m M acetone. The alpha5 integrin, keratinocyte growth factor receptor and cyclin D1 mRNA levels were higher compared to the controls as early as 2 h after ethanol and 30 min after acetone treatment of the cells. The stimulatory effect of ethanol and acetone on human keratinocytes may be one of the reasons why psoriasis can be precipitated by alcohol misuse.
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Alcohol and acetone produced by alcohol metabolism can promote proliferation of skin keratinocytes, thereby enhancing skin permeability and damaging its barrier function.
“While we cannot conclude completely on the harmful effect of alcohol on the body, alcohol and acetone produced by alcohol metabolism can promote the proliferation of skin keratinocytes, thereby enhancing skin permeability and damaging its barrier function.”
From Diet and Skin Aging—From the Perspective of Food Nutrition (2020) · cites this paper as [81]
Alcohol affects metabolism of triglycerides and cholesterol and affects lipid composition of the skin.
“Alcohol also affects the metabolism of triglycerides and cholesterol and affects the lipid composition of the skin [81,82].”
From Diet and Skin Aging—From the Perspective of Food Nutrition (2020) · cites this paper as [81]
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- Authors
- Farkas, �., Kem�ny, L., Sz�ll, M., Dobozy, A., & Bata-Cs�rgo, Z.
- Affiliations
- University of Szeged (HU)
- Corresponding author
- Farkas, �rp�d
- Venue
- Archives of Dermatological Research
- Published
- 2003-06-01
- Volume
- 295
- Issue
- 2
- Pages
- 56-62
- ISSN
- 0003-9187 (ISSN-L)
- 0340-3696
- 1432-069X
- OpenAlex
- W2072133488
- Cited by
- 69
- References
- 68
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 4 · 2023: 3 · 2022: 2 · 2021: 7 · 2020: 8 · 2019: 3 · 2018: 2 · 2017: 3 · 2015: 2 · 2014: 1 · 2013: 3 · 2012: 6
- FWCI
- 0.32
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Archives of Dermatological Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Hungarian Scientific Research Fund
- Magyar Tudományos Akadémia
- Bolyai Foundation
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Psoriasis: Treatment and Pathogenesis
- Health Sciences › Medicine › Pathology and Forensic Medicine › Alcohol Consumption and Health Effects
- Health Sciences › Medicine › Dermatology › Dermatology and Skin Diseases
- Keywords
- ethanol, acetone, keratinocyte proliferation, HaCaT keratinocytes, psoriasis, alcohol abuse, integrin alpha5, keratinocyte growth factor receptor, cyclin D1, gene expression
- MeSH terms
- Acetone, Ethanol, Cell Count, Cell Division, Cell Line, Transformed, Cell Survival, Humans, RNA, Messenger, Solvents, Keratinocytes, Receptors, Fibroblast Growth Factor, Cyclin D1, Integrin alpha5, Receptor, Fibroblast Growth Factor, Type 2
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