Multiplex PCR for rapid diagnosis of tuberculous meningitis
Abstract
Rapid and specific diagnosis of tubercular meningitis is of paramount importance to decrease morbidity and mortality. The aim of the study was to evaluate multiplex PCR using protein b, MPB 64, and IS6110 primers directed against M. tuberculosis complex for the diagnosis of tuberculous meningitis (TBM). Multiplex PCR was performed on 18 TBM confirmed cases (culture was positive), 92 clinically suspected TBM cases and 100 non-TBM (control group) patients. Multiplex PCR had a sensitivity of 94.4% for confirmed cases and specificity of 100% for confirmed TBM cases. In 92 clinically diagnosed but unconfirmed TBM cases, multiplex PCR was positive in 84.78% cases. The overall sensitivity of microscopy, culture and multiplex cases were 1.81, 16.73, and 86.63% and specificity was 100, 100, and 100% respectively. Multiplex PCR using protein b, MPB 64, and IS6110 primers has a high sensitivity and specificity in diagnosis of tubercular meningitis.
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Studies of IS6110 PCR for tuberculous meningitis reported sensitivity between 72% and 100% (median 91%) and specificity between 82% and 100% (median 99%).
“Several studies, with sample sizes ranging from 13 to 102 definite tuberculous meningitis patients and 17 to 127 controls, studied the performance of IS6110 PCR and reported a sensitivity between 72% and 100% (median 91%) with a specificity between 82% and 100% (median 99%) (73–78).”
From Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections (2024) · cites this paper as [76]
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Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections
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Self-citation
- Authors
- Kusum, S., Aman, S., Pallab, R., Kumar, S. S., Manish, M., Sudesh, P., Subhash, V., & Meera, S.
- Affiliations
- Post Graduate Institute of Medical Education and Research (IN)
- Venue
- Journal of Neurology
- Published
- 2011-03-31
- Volume
- 258
- Issue
- 10
- Pages
- 1781-1787
- ISSN
- 0012-1037 (ISSN-L)
- 0340-5354
- 1432-1459
- OpenAlex
- W2006679543
- Cited by
- 79
- References
- 28
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 6 · 2021: 1 · 2020: 5 · 2019: 7 · 2018: 4 · 2017: 8 · 2016: 8 · 2015: 9 · 2014: 9 · 2013: 10 · 2012: 9
- FWCI
- 9.50
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Journal of Neurology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Infectious Diseases and Tuberculosis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
- Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
- Keywords
- tuberculous meningitis, multiplex PCR, Mycobacterium tuberculosis complex, protein B, IS6110, rapid diagnosis, sensitivity and specificity, molecular diagnosis
- MeSH terms
- Adolescent, Adult, Aged, Aged, 80 and over, Child, Child, Preschool, DNA, Bacterial, Humans, Infant, Middle Aged, Mycobacterium tuberculosis, Sensitivity and Specificity, Tuberculosis, Meningeal, Young Adult, Multiplex Polymerase Chain Reaction
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