Cited by 28Pancreatic function and diabetesCalcium signaling and nucleotide metabolismIon channel regulation and function

A nutrient-regulated cytosolic calcium oscillator in endocrine pancreatic glucagon-secreting cells

Bode, H.-P., Weber, Stefanie, Fehmann, Hans-Christoph, Göke, Burkhard·1999·Pfl�gers Archiv European Journal of Physiology

Abstract

We investigated the influence of nutrients on spontaneous cytosolic calcium oscillations in InR1-G9 glucagonoma cells, a model for pancreatic alpha-cells. The oscillations depended on calcium release from stores and on calcium influx, partly through voltage-dependent calcium channels. Oscillations required the presence of at least 1 mM glucose, 50 microM alanine, or 50 microM glutamine, but were terminated by higher nutrient concentrations (40 mM glucose, or above 2 mM alanine or glutamine). The effects depended on the metabolism of the nutrients. Glutamine and alanine hyperpolarized the cells. This effect was inhibited (glutamine) or attenuated (alanine) by 1 mM ouabain. Our findings suggest that [Ca2+]i regulation in alpha-cells is dominated by slow oscillations induced by a lack of metabolic energy, resulting in decreased calcium export and storage, as well as increased calcium influx, partly due to depolarization caused by reduced sodium pump activity. These processes, leading to an elevated cytosolic calcium concentration, may mediate oscillations by calcium-induced calcium release from intracellular stores.

Integrity signals

Registry

Crossref · 10.1007/s004240050786

Cites

46 of 46 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Authors
Bode, H.-P., Weber, S., Fehmann, H.-C., & Göke, B.
Affiliations
  • University of Bern (CH)
  • Philipps University of Marburg (DE)
Venue
Pfl�gers Archiv European Journal of Physiology
Published
1999-01-18
Volume
437
Issue
3
Pages
324-334
ISSN
  • 0031-6768 (ISSN-L)
  • 1432-2013
OpenAlex
W2055769427
Cited by
28
References
46
Citations by year
2023: 1 · 2022: 1 · 2020: 1 · 2019: 2 · 2016: 1 · 2015: 2 · 2014: 5 · 2013: 4 · 2012: 2
FWCI
0.35
Type
journal article
Language
en
Open access
closed
Locations
  • Pflügers Archiv - European Journal of Physiology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
Keywords
cytosolic calcium oscillations, alpha cells, glucagon secretion, nutrient regulation, calcium store release, voltage-gated calcium channels, glucose, alanine, glutamine, membrane hyperpolarization, Na+/K+-ATPase, ouabain, calcium-induced calcium release
MeSH terms
Sodium-Potassium-Exchanging ATPase, Alanine, Animals, Calcium, Carbachol, Cytosol, Energy Metabolism, Glucagon, Glucagonoma, Glucose, Glutamine, Cricetinae, Homeostasis, Islets of Langerhans, Membrane Potentials, Pancreatic Neoplasms, Periodicity, Tumor Cells, Cultured, Muscarinic Agonists

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review