A nutrient-regulated cytosolic calcium oscillator in endocrine pancreatic glucagon-secreting cells
Abstract
We investigated the influence of nutrients on spontaneous cytosolic calcium oscillations in InR1-G9 glucagonoma cells, a model for pancreatic alpha-cells. The oscillations depended on calcium release from stores and on calcium influx, partly through voltage-dependent calcium channels. Oscillations required the presence of at least 1 mM glucose, 50 microM alanine, or 50 microM glutamine, but were terminated by higher nutrient concentrations (40 mM glucose, or above 2 mM alanine or glutamine). The effects depended on the metabolism of the nutrients. Glutamine and alanine hyperpolarized the cells. This effect was inhibited (glutamine) or attenuated (alanine) by 1 mM ouabain. Our findings suggest that [Ca2+]i regulation in alpha-cells is dominated by slow oscillations induced by a lack of metabolic energy, resulting in decreased calcium export and storage, as well as increased calcium influx, partly due to depolarization caused by reduced sodium pump activity. These processes, leading to an elevated cytosolic calcium concentration, may mediate oscillations by calcium-induced calcium release from intracellular stores.
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Glucose inhibits glucagon secretion from clonal glucagon-releasing cells.
“Glucose inhibits glucagon secretion from clonal glucagonreleasing cells [5,11,17]”
From Glucose inhibits glucagon secretion by a direct effect on mouse pancreatic alpha cells (2007) · cites this paper as [11]
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- Authors
- Bode, H.-P., Weber, S., Fehmann, H.-C., & Göke, B.
- Affiliations
- University of Bern (CH)
- Philipps University of Marburg (DE)
- ORCID
- Weber, Stefanie
- Venue
- Pfl�gers Archiv European Journal of Physiology
- Published
- 1999-01-18
- Volume
- 437
- Issue
- 3
- Pages
- 324-334
- ISSN
- 0031-6768 (ISSN-L)
- 1432-2013
- OpenAlex
- W2055769427
- Cited by
- 28
- References
- 46
- Citations by year
- 2023: 1 · 2022: 1 · 2020: 1 · 2019: 2 · 2016: 1 · 2015: 2 · 2014: 5 · 2013: 4 · 2012: 2
- FWCI
- 0.35
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Pflügers Archiv - European Journal of Physiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Keywords
- cytosolic calcium oscillations, alpha cells, glucagon secretion, nutrient regulation, calcium store release, voltage-gated calcium channels, glucose, alanine, glutamine, membrane hyperpolarization, Na+/K+-ATPase, ouabain, calcium-induced calcium release
- MeSH terms
- Sodium-Potassium-Exchanging ATPase, Alanine, Animals, Calcium, Carbachol, Cytosol, Energy Metabolism, Glucagon, Glucagonoma, Glucose, Glutamine, Cricetinae, Homeostasis, Islets of Langerhans, Membrane Potentials, Pancreatic Neoplasms, Periodicity, Tumor Cells, Cultured, Muscarinic Agonists
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