Sucrose synthase is involved in the conversion of sucrose to polysaccharides in filamentous nitrogen-fixing cyanobacteria
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Higher plants and cyanobacteria metabolize sucrose (Suc) by a similar set of enzymes. Suc synthase (SuS, UDP-glucose: D: -fructose 2-alpha-D: -glucosyl transferase, EC 2.4.1.13) catalyses the synthesis and cleavage of Suc, and in higher plants, it plays an important role in polysaccharides biosynthesis and carbon allocation. In this work, we have studied the functional relationship between SuS and the metabolism of polysaccharides in filamentous nitrogen-fixing cyanobacteria. We show that the nitrogen and carbon sources and light regulate the expression of the SuS encoding gene (susA), in a similar way that they regulate the accumulation of polysaccharides. Furthermore, glycogen content in an Anabaena sp. mutant strain with an insertion inactivation of susA was lower than in the wild type strain under diazotrophic conditions, while both glycogen and polysaccharides levels were higher in a mutant strain constitutively overexpressing susA. We also show that there are soluble and membrane-bound forms of SuS in Anabaena. Taken together, these results strongly suggest that SuS is involved in the Suc to polysaccharides conversion according to nutritional and environmental signals in filamentous nitrogen-fixing cyanobacteria.
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Sucrose synthase seems to be present only in filamentous N2-fixing cyanobacteria, where it is involved in the accumulation of storage and structural polysaccharides.
“This enzyme seems to be present only in filamentous N2-fixing cyanobacteria (Curatti et al. 2000; Salerno and Curatti 2003) where it is involved in the accumulation of storage (glycogen and sucrose) and structural (such as cellulose, see below) polysaccharides (Curatti et al. 2008).”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Curatti et al. 2008
Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302 · see citing works
Basic local alignment search tool
Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990) · Journal of Molecular Biology · doi:10.1006/jmbi.1990.9999 · Cited by 98,669 · see citing works
ABSORPTION OF LIGHT BY CHLOROPHYLL SOLUTIONS
Mackinney, G. (1941) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)51320-x · Cited by 4,018 · see citing works
Encyclopedia of plant physiology.
Rühland, W. (1958) · Cited by 2,708 · see citing works
Encyclopedia of plant physiology
Jelke, E. (1980) · Bioelectrochemistry and Bioenergetics · doi:10.1016/0302-4598(80)80054-7 · Cited by 2,426 · see citing works
Sucrose metabolism: regulatory mechanisms and pivotal roles in sugar sensing and plant development
Koch, K. E. (2004) · Current Opinion in Plant Biology · doi:10.1016/j.pbi.2004.03.014 · Cited by 1,487 · see citing works
Falkowski, P. G. (1997) · Nature · doi:10.1038/387272a0 · Cited by 1,308 · see citing works
Amor, Y., Haigler, C. H., Johnson, S., Wainscott, M., & Delmer, D. P. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.20.9353 · Cited by 655 · see citing works
Sederoff, H. W., & Huber, S. C. (2000) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.1080/10409230008984165 · Cited by 627 · see citing works
Zrenner, R. M., Salanoubat, M., Willmitzer, L., & Sonnewald, U. (1995) · The Plant Journal · doi:10.1046/j.1365-313x.1995.07010097.x · Cited by 536 · see citing works
Cai, Y., & Wölk, C. P. (1990) · Journal of Bacteriology · doi:10.1128/jb.172.6.3138-3145.1990 · Cited by 500 · see citing works
Hydrogenases and Hydrogen Photoproduction in Oxygenic Photosynthetic Organisms
Ghirardi, M. L., Posewitz, M. C., Maness, P., Dubini, A., Yu, J., & Seibert, M. (2006) · Annual Review of Plant Biology · doi:10.1146/annurev.arplant.58.032806.103848 · Cited by 371 · see citing works
Ting, C. S., Rocap, G., King, J., & Chisholm, S. W. (2002) · Trends in Microbiology · doi:10.1016/s0966-842x(02)02319-3 · Cited by 347 · see citing works
Carbon partitioning to cellulose synthesis
Haigler, C. H., Ivanova-Datcheva, M., Hogan, P. S., Salnikov, V. V., Hwang, S., Martin, K. A., & Delmer, D. P. (2001) · Plant Molecular Biology · doi:10.1023/a:1010615027986 · Cited by 337 · see citing works
Hydrogen production by Cyanobacteria
Dutta, D., De, D., Chaudhuri, S., & Bhattacharya, S. K. (2005) · Microbial Cell Factories · doi:10.1186/1475-2859-4-36 · Cited by 314 · see citing works
The enzymatic deficiency conditioned by the shrunken-1 mutations in maize
Chourey, P. S., & Nelson, O. E. (1976) · Biochemical Genetics · doi:10.1007/bf00485135 · Cited by 293 · see citing works
ADP-Glucose pyrophosphorylase, a regulatory enzyme for bacterial glycogen synthesis
Ballicora, M. A., Iglesias, A. A., & Preiss, J. (2003) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.67.2.213-225.2003 · Cited by 259 · see citing works
Origin of sucrose metabolism in higher plants: when, how and why?
Salerno, G. (2003) · Trends in Plant Science · doi:10.1016/s1360-1385(02)00029-8 · Cited by 202 · see citing works
Self-citationSucrose and Other Disaccharides
Avigad, G. (1982) · Springer eBooks · doi:10.1007/978-3-642-68275-9_7 · Cited by 198 · see citing works
Sederoff, H. W., & Huber, S. C. (2000) · Critical Reviews in Plant Sciences · doi:10.1080/07352680091139178 · Cited by 194 · see citing works
Sederoff, H. W., Huber, J. L. A., & Huber, S. C. (1997) · FEBS Letters · doi:10.1016/s0014-5793(97)01506-8 · Cited by 125 · see citing works
2D‐isolation of pure plasma and thylakoid membranes from the cyanobacteriumSynechocystissp. PCC 6803
Norling, B., Zak, E., Andersson, B., et al. (1998) · FEBS Letters · doi:10.1016/s0014-5793(98)01123-5 · Cited by 124 · see citing works
Cyanobacterial alkaline/neutral invertases. Origin of sucrose hydrolysis in the plant cytosol?
Vargas, W., Cumino, A. C., & Salerno, G. L. (2002) · Planta · doi:10.1007/s00425-002-0943-x · Cited by 117 · see citing works
Self-citationHeterocyst Formation inAnabaena
Wölk, C. P. (2014) · ASM Press eBooks · doi:10.1128/9781555818166.ch4 · Cited by 108 · see citing works
Tang, G.-Q., & Sturm, A. (1999) · Plant Molecular Biology · doi:10.1023/a:1006327606696 · Cited by 105 · see citing works
Regulatory effects of ammonia on carbon metabolism in photosynthesizing Chlorella pyrenoidosa
Kanazawa, T., Kirk, M. R., & Bassham, J. A. (1970) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/0005-2728(70)90106-4 · Cited by 103 · see citing works
Muñoz, F. J., Baroja‐Fernández, E., Morán-Zorzano, M. T., Viale, A. M., Etxeberria, E., Alonso-Casajús, N., & Pozueta‐Romero, J. (2005) · Plant and Cell Physiology · doi:10.1093/pcp/pci148 · Cited by 103 · see citing works
Evidence for plasma membrane-associated forms of sucrose synthase in maize
Carlson, S. J., Chourey, P. S., & Chourey, P. S. (1996) · Molecular and General Genetics MGG · doi:10.1007/bf02173776 · Cited by 97 · see citing works
Curatti, L., Flores, E., & Salerno, G. L. (2002) · FEBS Letters · doi:10.1016/s0014-5793(02)02283-4 · Cited by 95 · see citing works
Self-citationCumino, A. C., Marcozzi, C., Barreiro, R., & Salerno, G. L. (2007) · PLANT PHYSIOLOGY · doi:10.1104/pp.106.091736 · Cited by 89 · see citing works
Self-citationPorchia, A. C., & Salerno, G. L. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.24.13600 · Cited by 83 · see citing works
Self-citationMiao, X., Wu, Q., Wu, G., & Zhao, N. (2003) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2003.tb11500.x · Cited by 83 · see citing works
Interactions Between Carbon and Nitrogen Metabolism
Foyer, C. H., Ferrario, S., & Noctor, G. (2001) · Springer eBooks · doi:10.1007/978-3-662-04064-5_9 · Cited by 76 · see citing works
Modes of reduction of nitrogenase in heterocysts isolated from Anabaena species
Lockau, W., Peterson, R. B., Wölk, C. P., et al. (1978) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/0005-2728(78)90051-8 · Cited by 75 · see citing works
Promoting R & D in Photobiological Hydrogen Production Utilizing Mariculture-Raised Cyanobacteria
Sakurai, H., & Masukawa, H. (2007) · Marine Biotechnology · doi:10.1007/s10126-006-6073-x · Cited by 75 · see citing works
Heterotrophic micro- and macrocultures of a nitrogen-fixing cyanobacterium
Wölk, C. P., & Shaffer, P. W. (1976) · Archives of Microbiology · doi:10.1007/bf00690221 · Cited by 73 · see citing works
Mannan, R. M., & Pakrasi, H. B. (1993) · PLANT PHYSIOLOGY · doi:10.1104/pp.103.3.971 · Cited by 71 · see citing works
Cyanobacterial nitrogen metabolism and environmental biotechnology
Kumar, A. (1997) · Medical Entomology and Zoology · Cited by 68 · see citing works
Sowokinos, J. R., Spychalla, J. P., & Desborough, S. L. (1993) · PLANT PHYSIOLOGY · doi:10.1104/pp.101.3.1073 · Cited by 66 · see citing works
Porchia, A. C., Curatti, L., & Salerno, G. L. (1999) · Planta · doi:10.1007/s004250050651 · Cited by 65 · see citing works
Self-citationEtxeberria, E. (2003) · Journal of Experimental Botany · doi:10.1093/jxb/erg148 · Cited by 64 · see citing works
Komina, O., Zhou, Y., Sarath, G., et al. (2002) · PLANT PHYSIOLOGY · doi:10.1104/pp.002360 · Cited by 63 · see citing works
Factors influencing dark nitrogen fixation in a blue-green alga
Fay, P. K. (1976) · Applied and Environmental Microbiology · doi:10.1128/aem.31.3.376-379.1976 · Cited by 61 · see citing works
Tissue-specific expression of two genes for sucrose synthase in carrot (Daucus carota L.)
Sturm, A., Lienhard, S., Schatt, S., et al. (1999) · Plant Molecular Biology · doi:10.1023/a:1006199003756 · Cited by 60 · see citing works
Curatti, L., Folco, E. J. E., Desplats, P., et al. (1998) · Journal of Bacteriology · doi:10.1128/jb.180.24.6776-6779.1998 · Cited by 59 · see citing works
Self-citationFructose uptake and influence on growth of and nitrogen fixation by Anabaena variabilis
Haury, J. F., & Spiller, H. (1981) · Journal of Bacteriology · doi:10.1128/jb.147.1.227-235.1981 · Cited by 56 · see citing works
Cumino, A. C., Curatti, L., Giarrocco, L. E., et al. (2002) · FEBS Letters · doi:10.1016/s0014-5793(02)02516-4 · Cited by 53 · see citing works
Self-citationCuratti, L., Porchia, A. C., Herrera-Estrella, L., & Salerno, G. L. (2000) · Planta · doi:10.1007/s004250000343 · Cited by 49 · see citing works
Self-citationFructan metabolism in two species of Bromus subjected to chilling and water stress
Puebla, A., Salerno, G. L., & Pontis, H. G. (1997) · New Phytologist · doi:10.1111/j.1469-8137.1997.tb04738.x · Cited by 42 · see citing works
Self-citationERNST, A., & Böger, P. (1985) · Microbiology · doi:10.1099/00221287-131-12-3147 · Cited by 36 · see citing works
Curatti, L., Giarrocco, L. E., & Salerno, G. L. (2005) · Planta · doi:10.1007/s00425-005-0142-7 · Cited by 32 · see citing works
Self-citationWinfried, R. E. N. G., RIESSLAND, R., Scheibe, R., et al. (1993) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1993.tb18233.x · Cited by 31 · see citing works
Valiente, E. F., Nieva, M., Avendaño, M. C., et al. (1992) · Plant and Cell Physiology · doi:10.1093/oxfordjournals.pcp.a078255 · Cited by 28 · see citing works
Ammonia Induces Starch Degradation in Chlorella Cells
Miyachi, S., & Miyachi, S. (1985) · Plant and Cell Physiology · doi:10.1093/oxfordjournals.pcp.a076903 · Cited by 27 · see citing works
Reddy, P. M., Spiller, H., Albrecht, S. L., et al. (1996) · Applied and Environmental Microbiology · doi:10.1128/aem.62.4.1220-1226.1996 · Cited by 25 · see citing works
Lang, N. J., Krupp, J. M., & Koller, A. L. (1987) · Journal of Bacteriology · doi:10.1128/jb.169.2.920-923.1987 · Cited by 20 · see citing works
García‐González, M., Sivak, M. N., Guerrero, M. G., et al. (1992) · Physiologia Plantarum · doi:10.1111/j.1399-3054.1992.tb01331.x · Cited by 15 · see citing works
Zhu, Z., Sun, D., & Davidson, V. L. (1999) · Journal of Bacteriology · doi:10.1128/jb.181.20.6540-6542.1999 · Cited by 11 · see citing works
Torres, L. L., & Salerno, G. L. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0706709104 · Cited by 10 · see citing works
Self-citationJensen, B. (1990) · Archives of Microbiology · doi:10.1007/bf00249184 · Cited by 5 · see citing works
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Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering
Quintana, N., Van der Kooy, F., Van de Rhee, M. D., Voshol, G. P., & Verpoorte, R. (2011) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-011-3394-0 · Cited by 338 · see citing works
- Authors
- Curatti, L., Giarrocco, L. E., Cumino, A. C., & Salerno, G. L.
- Corresponding author
- Salerno, Graciela L.
- Venue
- Planta
- Published
- 2008-06-17
- Volume
- 228
- Issue
- 4
- Pages
- 617-625
- ISSN
- 0032-0935 (ISSN-L)
- 1432-2048
- OpenAlex
- W2061229641
- Cited by
- 41
- References
- 60
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 1 · 2023: 1 · 2021: 5 · 2019: 3 · 2018: 2 · 2017: 1 · 2016: 4 · 2015: 8 · 2014: 2 · 2013: 3 · 2012: 3
- FWCI
- 0.69
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- cc-by-nc-sa
- www.springer.com/tdm (tdm)
- Locations
- Planta (publishedVersion)
- PubMed (publishedVersion)
- CONICET Digital (CONICET) (submittedVersion, cc-by-nc-sa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
- Keywords
- sucrose synthase, sucrose metabolism, polysaccharide synthesis, glycogen, nitrogen-fixing cyanobacteria, Anabaena, carbon allocation, light regulation
- MeSH terms
- Quaternary Ammonium Compounds, Cloning, Molecular, Fructose, Glucosyltransferases, Light, Mutation, Nitrogen Fixation, Polysaccharides, Sucrose, Gene Expression, Gene Expression Regulation, Bacterial, Anabaena
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