Cited by 41Photosynthetic Processes and MechanismsPlant nutrient uptake and metabolismPolysaccharides and Plant Cell Walls

Sucrose synthase is involved in the conversion of sucrose to polysaccharides in filamentous nitrogen-fixing cyanobacteria

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Curatti, Leonardo, Giarrocco, Laura E., Cumino, Andrea C., Salerno, Graciela L.·2008·Planta

Abstract

Higher plants and cyanobacteria metabolize sucrose (Suc) by a similar set of enzymes. Suc synthase (SuS, UDP-glucose: D: -fructose 2-alpha-D: -glucosyl transferase, EC 2.4.1.13) catalyses the synthesis and cleavage of Suc, and in higher plants, it plays an important role in polysaccharides biosynthesis and carbon allocation. In this work, we have studied the functional relationship between SuS and the metabolism of polysaccharides in filamentous nitrogen-fixing cyanobacteria. We show that the nitrogen and carbon sources and light regulate the expression of the SuS encoding gene (susA), in a similar way that they regulate the accumulation of polysaccharides. Furthermore, glycogen content in an Anabaena sp. mutant strain with an insertion inactivation of susA was lower than in the wild type strain under diazotrophic conditions, while both glycogen and polysaccharides levels were higher in a mutant strain constitutively overexpressing susA. We also show that there are soluble and membrane-bound forms of SuS in Anabaena. Taken together, these results strongly suggest that SuS is involved in the Suc to polysaccharides conversion according to nutritional and environmental signals in filamentous nitrogen-fixing cyanobacteria.

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Crossref · 10.1007/s00425-008-0764-7

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  • Sucrose synthase seems to be present only in filamentous N2-fixing cyanobacteria, where it is involved in the accumulation of storage and structural polysaccharides.

    “This enzyme seems to be present only in filamentous N2-fixing cyanobacteria (Curatti et al. 2000; Salerno and Curatti 2003) where it is involved in the accumulation of storage (glycogen and sucrose) and structural (such as cellulose, see below) polysaccharides (Curatti et al. 2008).”

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Authors
Curatti, L., Giarrocco, L. E., Cumino, A. C., & Salerno, G. L.
Corresponding author
Salerno, Graciela L.
Venue
Planta
Published
2008-06-17
Volume
228
Issue
4
Pages
617-625
ISSN
  • 0032-0935 (ISSN-L)
  • 1432-2048
OpenAlex
W2061229641
Cited by
41
References
60
Citations by year
2026: 1 · 2025: 2 · 2024: 1 · 2023: 1 · 2021: 5 · 2019: 3 · 2018: 2 · 2017: 1 · 2016: 4 · 2015: 8 · 2014: 2 · 2013: 3 · 2012: 3
FWCI
0.69
Type
journal article
Language
en
Open access
Licence
Locations
  • Planta (publishedVersion)
  • PubMed (publishedVersion)
  • CONICET Digital (CONICET) (submittedVersion, cc-by-nc-sa)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
Keywords
sucrose synthase, sucrose metabolism, polysaccharide synthesis, glycogen, nitrogen-fixing cyanobacteria, Anabaena, carbon allocation, light regulation
MeSH terms
Quaternary Ammonium Compounds, Cloning, Molecular, Fructose, Glucosyltransferases, Light, Mutation, Nitrogen Fixation, Polysaccharides, Sucrose, Gene Expression, Gene Expression Regulation, Bacterial, Anabaena

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