A prokaryotic sucrose synthase gene ( susA ) isolated from a filamentous nitrogen-fixing cyanobacterium encodes a protein similar to those of plants
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Sucrose synthase (SS), a key enzyme in plant carbohydrate metabolism, has recently been isolated from Anabaena sp. strain PCC 7119, and biochemically characterized; two forms (SS-I and SS-II) were detected (Porchia et al. 1999, Planta 210: 34-40). The present study describes the first isolation and characterization of a prokaryotic SS gene, susA, encoding SS-II from that strain of Anabaena. A 7 kbp DNA fragment containing an open reading frame (EMBL accession number AJ010639) with about 30-40% amino acid identity with plant SSs was isolated from an Anabaena subgenomic library. The putative SS gene was demonstrated to encode an SS protein by expression in Escherichia coli. The biochemical properties of the recombinant enzyme were identical to those of the enzyme purified from the cyanobacterial cells. The deduced amino acid sequence of the Anabaena SS diverged from every plant SS reported. The occurrence of SS in cyanobacteria of different taxonomic groups was investigated. The enzyme occurs in several filamentous nitrogen-fixing cyanobacteria but not in two species of unicellular, non-diazotrophic cyanobacteria.
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Sucrose synthase seems to be present only in filamentous N2-fixing cyanobacteria, where it is involved in the accumulation of storage and structural polysaccharides.
“This enzyme seems to be present only in filamentous N2-fixing cyanobacteria (Curatti et al. 2000; Salerno and Curatti 2003) where it is involved in the accumulation of storage (glycogen and sucrose) and structural (such as cellulose, see below) polysaccharides (Curatti et al. 2008).”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Curatti et al. 2000
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Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering
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- Authors
- Curatti, L., Porchia, A. C., Herrera-Estrella, L., & Salerno, G. L.
- Venue
- Planta
- Published
- 2000-10-06
- Volume
- 211
- Issue
- 5
- Pages
- 729-735
- ISSN
- 0032-0935 (ISSN-L)
- 1432-2048
- OpenAlex
- W2021076298
- Cited by
- 49
- References
- 25
- Citations by year
- 2025: 1 · 2023: 2 · 2021: 2 · 2020: 2 · 2019: 2 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 4 · 2013: 1 · 2012: 3
- FWCI
- 6.15
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- cc-by-nc-sa
- www.springer.com/tdm (tdm)
- Locations
- Planta (publishedVersion)
- PubMed (publishedVersion)
- Conicet (submittedVersion, cc-by-nc-sa)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- sucrose synthase, cyanobacteria, nitrogen-fixing cyanobacteria, enzyme characterization, recombinant protein expression, amino acid sequence divergence, filamentous cyanobacteria
- MeSH terms
- Amino Acid Sequence, Cloning, Molecular, Genes, Bacterial, Glucosyltransferases, Kinetics, Molecular Sequence Data, Phylogeny, Plants, Recombinant Proteins, Substrate Specificity, Genomic Library, Polymerase Chain Reaction, Open Reading Frames, Sequence Alignment, Anabaena, Genes, Plant, Sequence Homology, Amino Acid
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