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Immunologic and metabolic characteristics of HPV-negative and HPV-positive head and neck squamous cell carcinomas are strikingly different

Krupar, Rosemarie, Robold, Katharina, Gaag, Doris, Spanier, Gerrit, Kreutz, Marina, Renner, Kathrin, Hellerbrand, Claus, Hofstaedter, Ferdinand, Bosserhoff, Anja K.·2014·Virchows Archiv
DOI 10.1007/s00428-014-1630-6
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Abstract

An HPV infection is involved in the etiology of about 25 % of head and neck squamous cell carcinomas (HNSCC). It has been postulated that a strong antitumoral immune response in HPV-positive tumors represents an important underlying mechanism for their good response to therapy. Recently, the Warburg phenomenon has returned to the center of attention because it affects antitumoral immune response and response to therapy. Accumulation of tumor cell-derived lactate inhibits cytotoxic T cells, as these, analogous to cancer cells, depend on glycolysis and lactate secretion for fulfillment of energy needs. Sparse information exists on the Warburg effect in HNSCC. This study aimed to characterize the metabolic and immunological features of HPV-negative and HPV-positive HNSCC. An immunohistochemical analysis of oropharyngeal carcinomas showed an enhanced antitumoral immune response (CD8/CD4 ratio) together with increased levels of proteins involved in transmembranous metabolite transportation (GLUT1 and CD147) and respiratory metabolism (COX5B) in HPV-positive tumors as compared to HPV-negative tumors. mRNA and Western blot analyses of an HPV-positive and HPV-negative HNSCC cell line revealed metabolic characteristics similar to the in vivo situation. Additionally, the HPV-negative cell line showed stronger extracellular lactate accumulation. In contrast, the HPV-positive cell line presented with better adaption to lactic acidosis suggesting an ability to metabolize lactate. Our resu…

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Metadata

Authors
Krupar, R., Robold, K., Gaag, D., Spanier, G., Kreutz, M., Renner, K., Hellerbrand, C., Hofstaedter, F., & Bosserhoff, A. K.
Venue
Virchows Archiv
Publisher
Springer Science and Business Media LLC
Published
2014-07-16
Volume
465
Issue
3
Pages
299-312
ISSN
  • 0945-6317
  • 1432-2307
DOI
10.1007/s00428-014-1630-6
Cited by
75
References
1
Language
en
Licence
www.springer.com/tdm (tdm)

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CoChat. (2026). Citation check: Immunologic and metabolic characteristics of HPV-negative and HPV-positive head and neck squamous cell carcinomas are strikingly different. https://app.cochat.ai/papers/10.1007/s00428-014-1630-6
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Krupar, R., Robold, K., Gaag, D., Spanier, G., Kreutz, M., Renner, K., Hellerbrand, C., Hofstaedter, F., & Bosserhoff, A. K. (2014). Immunologic and metabolic characteristics of HPV-negative and HPV-positive head and neck squamous cell carcinomas are strikingly different. Virchows Archiv. https://doi.org/10.1007/s00428-014-1630-6