Telomere shortening in gastric carcinoma with aging despite telomerase activation
Open accessAbstract
In the present study, we analyzed both telomere length and telomerase activity in surgical and autopsy samples of non-neoplastic mucosa and carcinomas of the stomach. Telomere length, determined by Southern blot analysis, demonstrated progressive shortening with age in non-neoplastic gastric mucosal specimens from 38 human subjects aged between 0 and 99 years, with an average annual loss rate of 46 base pairs (bp). The mean (+/- SD) telomere length in 21 gastric carcinomas was 7.0 +/- 1.6 x 10(3) base pairs (1.6 kbp). In 20 (95%) of the 21 subjects, the values were smaller than those in the nonneoplastic gastric mucosa (mean shortening 1.8 kbp), although a strong correlation was observed for the paired data (r = 0.69, P = 0.0004). Similarly, telomere lengths in carcinomas were shorter than those for intestinal metaplasia (a mean difference of 1.1 kbp). Telomerase activity, estimated using the telomeric repeat amplification protocol assay, was positive in 18 (86%) of the 21 gastric carcinomas, without significant differences among the three histological types (well, moderately, and poorly differentiated adenocarcinomas) or with sex or age. The results suggest that telomere length and possibly shortening rates vary with the individual, and that examination of both non-neoplastic mucosa and tumors is necessary to improve our understanding of the significance of telomerase in neoplasia.
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Shorter telomeres are frequently observed in cancer relative to adjacent normal tissue.
“Shorter telomeres are frequently observed in cancer relative to their adjacent normal tissue135–142.”
From Telomeres in cancer: tumour suppression and genome instability (2017) · cites this paper as [137]
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Telomeres in cancer: tumour suppression and genome instability
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- Authors
- Furugori, E., Hirayama, R., Nakamura, K.-I., Kammori, M., Esaki, Y., & Takubo, K.
- Affiliations
- Tokyo Metropolitan Institute of Gerontology (JP)
- Saitama Medical University (JP)
- The University of Tokyo (JP)
- ORCID
- Takubo, Kaiyo
- Venue
- Journal of Cancer Research and Clinical Oncology
- Published
- 2000-07-16
- Volume
- 126
- Issue
- 8
- Pages
- 481-485
- ISSN
- 0084-5353 (ISSN-L)
- 0171-5216
- 1432-1335
- PMC
- PMC12165231
- OpenAlex
- W2040591541
- Cited by
- 54
- References
- 13
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 3 · 2018: 2 · 2017: 2 · 2016: 2 · 2015: 1 · 2014: 1 · 2013: 2 · 2012: 2
- FWCI
- 2.46
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Journal of Cancer Research and Clinical Oncology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Gastric Cancer Management and Outcomes
- Keywords
- telomere shortening, gastric cancer, telomerase activity, aging, telomere length, gastric mucosa, intestinal metaplasia, adenocarcinoma, telomeric repeat amplification protocol
- MeSH terms
- Adenocarcinoma, Adult, Aged, Aged, 80 and over, Aging, Child, Preschool, Enzyme Activation, Female, Gastric Mucosa, Humans, Infant, Infant, Newborn, Male, Middle Aged, Polymorphism, Restriction Fragment Length, Stomach Neoplasms, Blotting, Southern, Telomere, Cellular Senescence, Telomerase, Terminal Repeat Sequences
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