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Isolation and characterization of cDNAs and genomic DNAs encoding ADP-glucose pyrophosphorylase large and small subunits from sweet potato
Abstract
Sweet potato [Ipomoea batatas (L.) Lam.], the world's seventh most important food crop, is also a major industrial raw material for starch and ethanol production. In the plant starch biosynthesis pathway, ADP-glucose pyrophosphorylase (AGPase) catalyzes the first, rate-limiting step and plays a pivotal role in regulating this process. In spite of the importance of sweet potato as a starch source, only a few studies have focused on the molecular aspects of starch biosynthesis in sweet potato and almost no intensive research has been carried out on the AGPase gene family in this species. In this study, cDNAs encoding two small subunits (SSs) and four large subunits (LSs) of AGPase isoforms were cloned from sweet potato and the genomic organizations of the corresponding AGPase genes were elucidated. Expression pattern analysis revealed that the two SSs were constitutively expressed, whereas the four LSs displayed differential expression patterns in various tissues and at different developmental stages. Co-expression of SSs with different LSs in Escherichia coli yielded eight heterotetramers showing different catalytic activities. Interactions between different SSs and LSs were confirmed by a yeast two-hybrid experiment. Our findings provide comprehensive information about AGPase gene sequences, structures, expression profiles, and subunit interactions in sweet potato. The results can serve as a foundation for elucidation of molecular mechanisms of starch synthesis in tuberous roots, and should contribute to future regulation of starch biosynthesis to improve sweet potato starch yield.
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A previous study showed that IbAGPb3 was only expressed in leaves and IbAGPb2 was expressed in sink tissues at low levels.
“This result is similar to the findings of a previous study that examined the expression of genes encoding AGPase subunits in sweet potato (Zhou et al., 2016), which showed that IbAGPb3 was only expressed in leaves and IbAGPb2 was expressed in sink tissues at low levels.”
From Comparative Transcriptome Analysis Reveals Critical Function of Sucrose Metabolism Related-Enzymes in Starch Accumulation in the Storage Root of Sweet Potato (2017) · cites this paper as Zhou et al., 2016
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Metadata
- Authors
- Zhou, Y.-X., Chen, Y.-X., Tao, X., Cheng, X.-J., & Wang, H.-Y.
- Affiliations
- Chinese Academy of Sciences (CN)
- ORCID
- Tao, Xiang
- Venue
- Molecular Genetics and Genomics
- Published
- 2015-10-24
- Volume
- 291
- Issue
- 2
- Pages
- 609-620
- ISSN
- 1617-4615 (ISSN-L)
- 1617-4623
- OpenAlex
- W1815575374
- Cited by
- 19
- References
- 40
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 3 · 2023: 3 · 2022: 3 · 2021: 1 · 2020: 1 · 2019: 1 · 2017: 3
- FWCI
- 1.21
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Molecular Genetics and Genomics (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Glycogen Storage Diseases and Myoclonus
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- ADP-glucose pyrophosphorylase, Ipomoea batatas, starch biosynthesis, cDNA cloning, gene structure, tissue-specific gene expression, subunit interactions, tuberous roots, starch yield
- MeSH terms
- Cloning, Molecular, Starch, Sequence Homology, Amino Acid, DNA, Complementary, Gene Expression Regulation, Plant, Plant Roots, Protein Subunits, Ipomoea batatas, Glucose-1-Phosphate Adenylyltransferase
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