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Presence of Multiple cDNAs Encoding an Isoform of ADP-Glucose Pyrophosphorylase Large Subunit from Sweet Potato and Characterization of Expression Levels
Abstract
Three cDNAs (iAGPLI-1, iAGPLI-2, and iAGPLI-3) encoding an isoform of AGPase large subunit were isolated from a sweet potato cDNA library constructed from tuberous root tissue. iAGPLI-1 was 2,161 bp in length and contained an open reading frame of 517 amino acids with a calculated molecular mass of 57,689 Da. iAGPLI-2 and iAGPLI-3 were 1,804 and 1,524 bp in length, respectively, and contained partial open reading frames of 490 and 385 amino acids. Deduced amino acid sequence comparison analysis showed that iAGPLI-1 has sequence identity with iAGPLI-2 (97.9) and iAGPLI-3 (98.7%) while iAGPLI-2 and iAGPLI-3 have 96.8% sequence identity. iAGPLI-1 had the highest sequence identity of 77.8% with potato AGPase (sAGPL1). Steady-state levels of iAGPLI-1 transcripts were expressed predominantly in the stem, and moderately in the tuberous root, but not in either the roots or leaves. However, AGPase activity was present in all tissues. The expression level in the stem declined dramatically after a 12 h incubation in the dark to nearly 3% of the value under light, although the activity under a dark condition remained at half the levels in light. The activity levels were not correlated with the transcript levels. iAGPL transcripts in leaves were induced by sucrose feeding but not by glucose or fructose. Therefore, the expression of iAGPLI-1 is regulated in stem tissue preferentially and by sucrose. Southern blot analysis showed that the sweet potato genome contained several copies of iAGPLI gene probably due to polyploidy.
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In sweet potato, the key enzymes involved in starch biosynthesis, including AGPase, GBSS, ISA, SBE, SP, and SSS, and their associated genes have been isolated and studied.
“In sweet potato, the key enzymes involved in starch biosynthesis, including ADP-glucose pyrophosphorylase (AGPase, EC 2.7.7.27), granule-bound starch synthases (GBSS, EC 2.4.1.21), isoamylase (ISA, EC 3.2.1.68), starch-branching enzyme (SBE, EC 2.4.1.18), starch phosphorylase (SP, EC 2.4.1.1), and soluble starch synthase (SSS, EC 2.4.1.21), and their associated genes have been isolated and studied”
From Comparative Transcriptome Analysis Reveals Critical Function of Sucrose Metabolism Related-Enzymes in Starch Accumulation in the Storage Root of Sweet Potato (2017) · cites this paper as Harn et al., 2000
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Metadata
- Authors
- Harn, C. H., Bae, J. M., Lee, S. S., Min, S. R., & Liu, J. R.
- Affiliations
- Nongwoo Bio (South Korea) (KR)
- ORCID
- Min, Sung Ran
- Venue
- Plant and Cell Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2000-11-15
- Volume
- 41
- Issue
- 11
- Pages
- 1235-1242
- ISSN
- 0032-0781 (ISSN-L)
- 1471-9053
- OpenAlex
- W2109074392
- Cited by
- 38
- References
- 27
- Citations by year
- 2024: 2 · 2023: 2 · 2022: 1 · 2021: 1 · 2020: 1 · 2018: 4 · 2017: 2 · 2016: 4 · 2015: 2 · 2014: 3 · 2013: 1 · 2012: 2
- FWCI
- 2.79
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Plant and Cell Physiology (publishedVersion)
- PubMed (publishedVersion)
- KRIBB Repository (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Glycogen Storage Diseases and Myoclonus
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
- Keywords
- ADP-glucose pyrophosphorylase, Ipomoea batatas, cDNA cloning, gene expression, light regulation, tuberous roots, stem tissue, polyploidy, gene family, starch biosynthesis
- MeSH terms
- Amino Acid Sequence, Isoenzymes, Molecular Sequence Data, Nucleotidyltransferases, Tissue Distribution, Blotting, Southern, Blotting, Northern, Gene Expression Regulation, Enzymologic, Sequence Alignment, Sequence Homology, Amino Acid, Sequence Analysis, DNA, DNA, Complementary, Gene Expression Regulation, Plant, Gene Expression Regulation, Developmental, Gene Dosage, DNA, Plant, RNA, Plant, Solanaceae, Protein Subunits, Glucose-1-Phosphate Adenylyltransferase
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