The DOI matches its Crossref record; 74 of the 74 references it cites were found in a registry or an index.
Arbuscular mycorrhiza increase artemisinin accumulation in Artemisia annua by higher expression of key biosynthesis genes via enhanced jasmonic acid levels
Abstract
It is becoming increasingly evident that the formation of arbuscular mycorrhiza (AM) enhances secondary metabolite production in shoots. Despite mounting evidence, relatively little is known about the underlying mechanisms. This study suggests that increase in artemisinin concentration in Artemisia annua colonized by Rhizophagus intraradices is due to altered trichome density as well as transcriptional patterns that are mediated via enhanced jasmonic acid (JA) levels. Mycorrhizal (M) plants had higher JA levels in leaf tissue that may be due to induction of an allene oxidase synthase gene (AOS), encoding one of the key enzymes for JA production. Non-mycorrhizal (NM) plants were exogenously supplied with a range of methyl jasmonic acid concentrations. When leaves of NM and M plants with similar levels of endogenous JA were compared, these matched closely in terms of shoot trichome density, artemisinin concentration, and transcript profile of artemisinin biosynthesis genes. Mycorrhization increased artemisinin levels by increasing glandular trichome density and transcriptional activation of artemisinin biosynthesis genes. Transcriptional analysis of some rate-limiting enzymes of mevalonate and methyl erythritol phosphate (MEP) pathways revealed that AM increases isoprenoids by induction of the MEP pathway. A decline in artemisinin concentration in shoots of NM and M plants treated with ibuprofen (an inhibitor of JA biosynthesis) further confirmed the implication of JA in the mechanism of artemisinin production.
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“Mandal et al. (2015) showed that A. annua mutualism with AM fungus Rhizophagus intraradices results in increased artemisinin accumulation due to enhanced expression of certain artemisinin biosynthesis genes, namely ADS, CYP71AV1, ALDH1 and DBR2 mediated through enhanced JA levels, most probably due to stimulation of the allene oxide synthase gene.”
From Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches (2021) · cites this paper as Mandal et al. 2015
In the citing paper
“The mycorrhizal infection increased glandular trichome density and transcriptional activation of artemisinin biosynthesis genes.”
From Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches (2021) · cites this paper as Mandal et al. 2015
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Cites
A formaldehyde - glutaraldehyde fixative of high osmolarity for use in electron microscopy
Karnovsky, M. J., & Karnovsky, M. J. (1965) · The Journal of Cell Biology · Cited by 9,412
Phillips, J. M., & Hayman, D. S. (1970) · Transactions of the British Mycological Society · doi:10.1016/s0007-1536(70)80110-3 · Cited by 8,587
AN EVALUATION OF TECHNIQUES FOR MEASURING VESICULAR ARBUSCULAR MYCORRHIZAL INFECTION IN ROOTS
Giovannetti, M., & Mosse, B. (1980) · New Phytologist · doi:10.1111/j.1469-8137.1980.tb04556.x · Cited by 5,925
A formaldehyde - glutaraldehyde fixative of high osmolarity for use in electron microscopy
Karnovsky, M. J. (1965) · The Journal of Cell Biology · Cited by 5,882
Chemical analysis of ecological materials.
Allen, S. E. (1974) · Blackwell Scientific Publications eBooks · Cited by 4,375
Production of the antimalarial drug precursor artemisinic acid in engineered yeast
Ro, D.-K., Paradise, E. M., Ouellet, M., Fisher, K. J., Newman, K. L., Ndungu, J. M., Ho, K. A., Eachus, R. A., Ham, T. S., Kirby, J., et al. (2006) · Nature · doi:10.1038/nature04640 · Cited by 2,805
Wasternack, C., & Hause, B. (2013) · Annals of Botany · doi:10.1093/aob/mct067 · Cited by 2,629
Plant sesquiterpenes induce hyphal branching in arbuscular mycorrhizal fungi
Akiyama, K., Matsuzaki, K.-I., & Hayashi, H. (2005) · Nature · doi:10.1038/nature03608 · Cited by 2,429
Elicitor signal transduction leading to production of plant secondary metabolites
Zhao, J. H., Davis, L. C., & Verpoorte, R. (2005) · Biotechnology Advances · doi:10.1016/j.biotechadv.2005.01.003 · Cited by 1,985
Natural products and plant disease resistance
Dixon, R. A. (2001) · Nature · doi:10.1038/35081178 · Cited by 1,796
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Cao, G.-H., Li, Z.-D., Wang, X.-F., Zhang, X., Zhao, R.-H., Gu, W., Chen, D., Yu, J., & He, S. (2020) · Figshare · doi:10.6084/m9.figshare.12015447
Zúñiga-Silgado, D., Cossio-Marín, E., Silva-Durango, A. D., Cesario-Solis, M. E., Reyes-Plaza, L. M., & Sarmiento-López, L. G. (2026) · Rhizosphere · doi:10.1016/j.rhisph.2026.101475
Liu, Y., Lv, Y., Jian, J., Meng, L., & Yan, J. (2026) · Mycorrhiza · doi:10.1007/s00572-026-01318-8
Recent advances in microbial regulation of plant secondary metabolism
Bai-Han, G. A. O., Duan, B., & Xue, Q. I. A. O. (2026) · Shengming kexue · doi:10.3724/cbls.2026087
Metadata
- Authors
- Mandal, S., Upadhyay, S., Wajid, S., Ram, M., Jain, D. C., Singh, V. P., Abdin, M. Z., & Kapoor, R.
- Affiliations
- University of Delhi (IN)
- Jamia Hamdard (IN)
- Ipca Laboratories (India) (IN)
- Corresponding author
- Kapoor, Rupam
- Venue
- Mycorrhiza
- Published
- 2014-11-05
- Volume
- 25
- Issue
- 5
- Pages
- 345-357
- ISSN
- 0940-6360 (ISSN-L)
- 1432-1890
- OpenAlex
- W2009545804
- Cited by
- 111
- References
- 74
- Citations by year
- 2026: 6 · 2025: 4 · 2024: 8 · 2023: 13 · 2022: 26 · 2021: 11 · 2020: 8 · 2019: 4 · 2018: 12 · 2017: 8 · 2016: 5 · 2015: 6
- FWCI
- 2.60
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Mycorrhiza (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Mycorrhizal Fungi and Plant Interactions
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Plant and fungal interactions
- Keywords
- arbuscular mycorrhizal symbiosis, artemisinin biosynthesis, Artemisia annua, Glomus intraradices, jasmonic acid, glandular trichomes, trichome density, allene oxide synthase, methyl jasmonic acid, mevalonate pathway, methylerythritol phosphate pathway, terpenoids, secondary metabolite production, gene expression, ibuprofen
- MeSH terms
- Cyclopentanes, Plant Proteins, Gene Expression Regulation, Plant, Plant Leaves, Plant Roots, Intramolecular Oxidoreductases, Artemisia annua, Artemisinins, Mycorrhizae, Biosynthetic Pathways, Oxylipins
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