The jasmonate‐responsive Aa MYC 2 transcription factor positively regulates artemisinin biosynthesis in Artemisia annua
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Summary: The plant Artemisia annua is well known due to the production of artemisinin, a sesquiterpene lactone that is widely used in malaria treatment. Phytohormones play important roles in plant secondary metabolism, such as jasmonic acid (JA), which can induce artemisinin biosynthesis in A. annua. Nevertheless, the JA‐inducing mechanism remains poorly understood. The expression of gene Aa MYC 2 was rapidly induced by JA and Aa MYC 2 binds the G‐box‐like motifs within the promoters of gene CYP 71 AV 1 and DBR 2, which are key structural genes in the artemisinin biosynthetic pathway. Overexpression of Aa MYC 2 in A. annua significantly activated the transcript levels of CYP 71 AV 1 and DBR 2, which resulted in an increased artemisinin content. By contrast, artemisinin content was reduced in the RNA i transgenic A. annua plants in which the expression of Aa MYC 2 was suppressed. Meanwhile, the RNA i transgenic A. annua plants showed lower sensitivity to methyl jasmonate treatment than the wild‐type plants. These results demonstrate that Aa MYC 2 is a positive regulator of artemisinin biosynthesis and is of great value in genetic engineering of A. annua for increased artemisinin production.
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Overexpression of AaMYC2 increased expression of DBR2 and CYP71AV1 involved in artemisinin biosynthesis and AaMYC2 suppression decreased artemisinin biosynthesis.
“Using RNAi, overexpression of AaMYC2 increased expression of DBR2 and CYP71AV1 involved in artemisinin biosynthesis and AaMYC2 suppression decreased artemisinin biosynthesis (Shen et al. 2016).”
From Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches (2021) · cites this paper as Shen et al. 2016
The ‘AaMYC2 promotes artemisinin biosynthesis’ section (Fig. 5b, 5f) reports that overexpression increased DBR2 and CYP71AV1 transcripts, while AaMYC2 suppression reduced artemisinin content. The methods are conflated in the citing sentence: the paper used RNAi to suppress AaMYC2, not to overexpress it; overexpression used a CaMV35S-driven construct. (LLM, conf=0.86)
Experimental — accuracy not yet measuredJA application induces artemisinin biosynthesis by increasing expression of AaMYC2, a transcription factor that binds to the DBR2 and CYP71AV1 promoters.
“JA application induces artemisinin biosynthesis by increasing expression of AaMYC2 , a transcription factor that binds to the DBR2 and CYP71AV1 promoters.”
From Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches (2021) · cites this paper as Shen et al. 2016
In A. annua, two bHLH transcription factors, AabHLH1 and AaMYC2, are positive regulators of artemisinin biosynthesis.
“In A. annua two bHLH transcription factors, AabHLH1 (Ji et al. 2014) and AaMYC2 (Shen et al. 2016) are positive regulators of artemisinin biosynthesis suggesting they may play a role in artemisinin regulation in response to cold stress.”
From Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches (2021) · cites this paper as Shen et al. 2016
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- Authors
- Shen, Q., Lu, X., Yan, T., Fu, X., Lv, Z., Zhang, F., Pan, Q., Wang, G., Sun, X., & Tang, K.
- Affiliations
- Shanghai Jiao Tong University (CN)
- China Pharmaceutical University (CN)
- Corresponding author
- Tang, Kexuan
- ORCID
- Venue
- New Phytologist
- Publisher
- Wiley
- Published
- 2016-02-10
- Volume
- 210
- Issue
- 4
- Pages
- 1269-1281
- ISSN
- 0028-646X (ISSN-L)
- 1469-8137
- OpenAlex
- W2279195969
- Cited by
- 296
- References
- 54
- Citations by year
- 2026: 23 · 2025: 30 · 2024: 34 · 2023: 32 · 2022: 34 · 2021: 53 · 2020: 22 · 2019: 27 · 2018: 23 · 2017: 9 · 2016: 9
- FWCI
- 11.78
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- New Phytologist (publishedVersion)
- PubMed (publishedVersion)
- Funders
- China National High-Tech ‘863’ program (2011AA100605)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant Gene Expression Analysis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Sesquiterpenes and Asteraceae Studies
- Keywords
- artemisinin biosynthesis, Artemisia annua, MYC transcription factors, jasmonic acid, methyl jasmonate, CYP71AV1, sesquiterpene lactone biosynthesis, secondary metabolism regulation
- MeSH terms
- Acetates, Cyclopentanes, Plant Growth Regulators, Plant Proteins, Promoter Regions, Genetic, Transcription Factors, Gene Expression, Plants, Genetically Modified, Artemisia annua, RNA Interference, Artemisinins, Biosynthetic Pathways, Oxylipins
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