Acute hyperglycemia and acute hyperinsulinemia decrease plasma fibrinolytic activity and increase plasminogen activator inhibitor type 1 in the rat

Pandolfi, A.; Giaccari, A.; Cilli, C.; Alberta, M. M.; Morviducci, L.; De Filippis, E. A.; Buongiorno, A.; Pellegrini, G.; Capani, G.; Consoli, A.

doi:10.1007/s005920170016

Cited by 144Blood Coagulation and Thrombosis MechanismsBlood properties and coagulationAdipokines, Inflammation, and Metabolic Diseases

Acute hyperglycemia and acute hyperinsulinemia decrease plasma fibrinolytic activity and increase plasminogen activator inhibitor type 1 in the rat

Pandolfi, A., Giaccari, A., Cilli, C., Alberta, M. M., Morviducci, L., De Filippis, E. A., Buongiorno, A., Pellegrini, G. +2 more·2001·Acta Diabetologica

DOI 10.1007/s005920170016

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Abstract

Decreased plasma fibrinolysis may contribute to accelerated atherothrombosis in diabetes. To observe whether hyperglycemia and hyperinsulinemia, common findings in type 2 diabetes, acutely affect plasma fibrinolysis in vivo, we evaluated plasma fibrinolysis (lysis of fibrin plates, free PAI-1 activity and t-PA activity) in the rat after a hyperglycemic euinsulinemic clamp (n=8), an euglycemic hyperinsulinemic clamp (n=7) or a saline infusion (n=15). Plasma fibrinolytic activity was sharply reduced after both the hyperglycemic and hyperinsulinemic clamps as compared to the respective controls (mean lysis areas on the fibrin plate, 139+/-21 vs. 323+/-30 mm2, p<0.001; 78+/-27 vs. 312+/-27 mm2 p<0.001, respectively). Plasma PAI-1 activity was greater after both hyperglycemic and hyperinsulinemic clamps as compared to saline infusion (6.6+/-2.6 vs. 1.6+/-0.6 IU/ml, p<0.001; 26+/-4 vs. 1.3+/-0.7 IU/ml, p<0.0001, respectively). Plasma t-PA activity was significantly reduced both after the hyperglycemic (0.36+/-0.15 vs. 2.17+/-0.18 IU/ml in controls, p<0.001) and the hyperinsulinemic (0.3+/-0.1 vs. 2.3+/-0.3 IU/ml in control, p<0.001) clamps. These data show that in vivo both acute hyperglycemia and acute hyperinsulinemia can decrease plasma fibrinolytic potential and that this is due to increased plasma PAI-1 and decreased free t-PA activities.

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Acute glucose and insulin administration decrease t-PA activity in the rat.
“Acute glucose and insulin administration decrease t-PA activity in the rat.”
From The Vasculature in Prediabetes (2018) · cites this paper as [132]

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Authors: Pandolfi, A., Giaccari, A., Cilli, C., Alberta, M. M., Morviducci, L., De Filippis, E. A., Buongiorno, A., Pellegrini, G., Capani, G., & Consoli, A.
Affiliations: University of Chieti-Pescara (IT)
Università Cattolica del Sacro Cuore (IT)
Istituto Superiore di Sanità (IT)
ORCID: Pandolfi, A.
Giaccari, A.
Consoli, A.
Venue: Acta Diabetologica
Publisher: Springer Science and Business Media LLC
Published: 2001-06-01
Volume: 38
Issue: 2
Pages: 71-76
ISSN: 0940-5429 (ISSN-L)
DOI: 10.1007/s005920170016
OpenAlex: W2044594443
Cited by: 144
References: 30
Citations by year: 2026: 2 · 2025: 6 · 2024: 6 · 2023: 9 · 2022: 5 · 2021: 8 · 2020: 10 · 2019: 3 · 2018: 6 · 2017: 8 · 2016: 3 · 2015: 8 · 2014: 6 · 2013: 6 · 2012: 8
FWCI: 1.27
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Acta Diabetologica (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health
Topics: Health Sciences › Medicine › Hematology › Blood Coagulation and Thrombosis Mechanisms
Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Blood properties and coagulation
Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
Keywords: hyperglycemia, hyperinsulinemia, plasma fibrinolysis, plasminogen activator inhibitor-1, tissue plasminogen activator, type 2 diabetes, atherothrombosis, hyperinsulinemic-euglycemic clamp, hyperglycemic clamp
MeSH terms: Animals, Diabetes Mellitus, Type 2, Fibrinolysis, Glucose, Hyperglycemia, Hyperinsulinism, Insulin, Male, Tissue Plasminogen Activator, Glucose Clamp Technique, Rats, Sprague-Dawley, Plasminogen Activator Inhibitor 1, Rats

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Acute hyperglycemia and acute hyperinsulinemia decrease plasma fibrinolytic activity and increase plasminogen activator inhibitor type 1 in the rat

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