Correlation of low-density lipoprotein modification by myeloperoxidase with hypochlorous acid formation
Abstract
Myeloperoxidase is an enzyme in phagocytes which catalyzes several redox reactions. A major product is hypochlorous acid which appears to be important in inflammatory processes such as atherosclerosis. The aim of this study was to investigate whether the kinetics of low-density lipoprotein modification by the myeloperoxidase/hydrogen peroxide/chloride system in vitro conform to the established kinetics of hypochlorous acid formation and to compare the results with known in vivo data. The absorbance at 234 nm was applied to study the kinetics of the modification of low-density lipoprotein. Variation of the concentration of low-density lipoprotein, hydrogen peroxide, and chloride, respectively, had a biphasic effect on the maximal rate of low-density lipoprotein modification. Increasing the substrates up to certain threshold levels resulted in increased modification, however, further increases caused inhibition of low-density lipoprotein modification. The inhibitory effect of higher low-density lipoprotein concentrations might be relevant, since these concentrations occur in the human aortic intima. Furthermore, a positive correlation was found between the maximal rate of low-density lipoprotein modification and the acidity of the medium. In summary, low-density lipoprotein modification is affected by the myeloperoxidase/hydrogen peroxide/chloride system in a similar manner to hypochlorous acid production. We conclude that myeloperoxidase, which has been detected in atherosclerotic lesions, is able to modify low-density lipoprotein into the form which is taken up by macrophages in an uncontrolled manner.
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In the context of atherosclerosis, HOCl has been shown to oxidize both high and low density lipoproteins.
“In the context of atherosclerosis, HOCl has been shown to oxidize both high and low density lipoproteins (90, 470, 881, 1042).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [470]
Cites
The pathogenesis of atherosclerosis: a perspective for the 1990s
Ross, R. (1993) · Nature · doi:10.1038/362801a0 · Cited by 10,618 · see citing works
A Receptor-Mediated Pathway for Cholesterol Homeostasis
Brown, M. S., & Goldstein, J. L. (1986) · Science · doi:10.1126/science.3513311 · Cited by 5,703 · see citing works
Biochemistry and pathology of radical-mediated protein oxidation
Dean, R. T., Fu, S., Stocker, R., & Davies, M. J. (1997) · Biochemical Journal · doi:10.1042/bj3240001 · Cited by 1,678 · see citing works
Daugherty, A., Dunn, J. L., Rateri, D. L., & Heinecke, J. W. (1994) · Journal of Clinical Investigation · doi:10.1172/jci117342 · Cited by 1,269 · see citing works
Presence of hypochlorite-modified proteins in human atherosclerotic lesions.
Hazell, L. J., Arnold, L., Flowers, D., Waeg, G., Malle, E., & Stocker, R. (1996) · Journal of Clinical Investigation · doi:10.1172/jci118576 · Cited by 592 · see citing works
Heinecke, J. W., Li, W., Daehnke, H. L., & Goldstein, J. A. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)53581-x · Cited by 485 · see citing works
Arterial foam cells with distinctive immunomorphologic and histochemical features of macrophages.
Schaffner, T., Taylor, K., Bartucci, E. J., Fischer-Dzoga, K., Beeson, J. H., Glagov, S. K., & Wissler, R. W. (1980) · PubMed Central · Cited by 475 · see citing works
Tyrosyl radical generated by myeloperoxidase catalyzes the oxidative cross-linking of proteins.
Heinecke, J. W., Li, W., Francis, G. A., & Goldstein, J. A. (1993) · Journal of Clinical Investigation · doi:10.1172/jci116531 · Cited by 354 · see citing works
Kleinveld, H. A., Hak-Lemmers, H. L. M., Stalenhoef, A. F. H., & Demacker, P. N. M. (1992) · Clinical Chemistry · doi:10.1093/clinchem/38.10.2066 · Cited by 348 · see citing works
Savenkova, M. L., Mueller, D. M., & Heinecke, J. W. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)32005-7 · Cited by 339 · see citing works
The Reticuloendothelial System
Friedman, H. P., Escobar, M., Reichard, S. M., & Filkins, J. P. (1985) · Springer eBooks · doi:10.1007/978-1-4613-2353-2 · Cited by 314 · see citing works
Biochemistry and Physiology of the Neutrophil
Edwards, S. W. (1994) · Cambridge University Press eBooks · doi:10.1017/cbo9780511608421 · Cited by 286 · see citing works
Hazell, L. J., van den Berg, J. J. M., & Stocker, R. (1994) · Biochemical Journal · doi:10.1042/bj3020297 · Cited by 267 · see citing works
Chlorohydrin Formation From Unsaturated Fatty Acids Reacted With Hypochlorous Acid
Winterbourn, C. C., van den Berg, J. J. M., Roitman, E., & Kuypers, F. A. (1992) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(92)90609-z · Cited by 258 · see citing works
Hazen, S. L., Hsu, F. F., Duffin, K., & Heinecke, J. W. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.38.23080 · Cited by 221 · see citing works
Neutrophils Kill Pulmonary Endothelial Cells by a Hydrogen-Peroxide-Dependent Pathway
Martin, W. J. (1984) · American Review of Respiratory Disease · doi:10.1164/arrd.1984.130.2.209 · Cited by 206 · see citing works
Weening, R. S., Roos, D., & Loos, J. A. (1974) · PubMed · Cited by 201 · see citing works
Aruoma, O. I., & Halliwell, B. B. (1987) · Biochemical Journal · doi:10.1042/bj2480973 · Cited by 179 · see citing works
Myeloperoxidase of Human Leukaemic Leucocytes
Zgliczyński, J. M., Stelmaszyńska, T., Ostrowski, W., Naskalski, J. W., & Sznajd, J. (1968) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1968.tb00246.x · Cited by 169 · see citing works
Smith, E. B., & Staples, E. M. (1980) · Atherosclerosis · doi:10.1016/0021-9150(80)90065-9 · Cited by 167 · see citing works
Winterbourn, C. C., Garcia, R. C., & Segal, A. W. (1985) · Biochemical Journal · doi:10.1042/bj2280583 · Cited by 153 · see citing works
Heinecke, J. W., Li, W., Mueller, D. M., Bohrer, A., & TURK, J. W. (1994) · Biochemistry · doi:10.1021/bi00199a041 · Cited by 141 · see citing works
Francis, G. A., Mendez, A. J., Bierman, E. L., & Heinecke, J. W. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.14.6631 · Cited by 129 · see citing works
Does an acidic pH explain why low density lipoprotein is oxidised in atherosclerotic lesions?
Leake, D. S. (1997) · Atherosclerosis · doi:10.1016/s0021-9150(96)06035-2 · Cited by 116 · see citing works
Development of intimal lesions after leukocyte migration into the vascular wall.
PRESCOTT, M. F., McBride, C. K., & Court, M. (1989) · PubMed · Cited by 115 · see citing works
Andrews, P. C., & Krinsky, N. I. (1982) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)33436-7 · Cited by 107 · see citing works
Omeprazole attenuates oxygen-derived free radical production from human neutrophils
Suzuki, M., Mori, M., Miura, S., Suematsu, M., Fukumura, D., Kimura, H., & Ishii, H. (1996) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(96)00180-3 · Cited by 106 · see citing works
(1989) · De Gruyter eBooks · doi:10.1515/9783110873665 · Cited by 103 · see citing works
Possible involvement of myeloperoxidase in lipid peroxidation
Stelmaszyńska, T., Kukovetz, E. M., Egger, G., & Schaur, R. J. (1992) · International Journal of Biochemistry · doi:10.1016/0020-711x(92)90237-u · Cited by 87 · see citing works
Self-citationInfluence of superoxide on myeloperoxidase kinetics measured with a hydrogen peroxide electrode
Kettle, A. J., & Winterbourn, C. C. (1989) · Biochemical Journal · doi:10.1042/bj2630823 · Cited by 66 · see citing works
Panasenko, O. M., Evgina, S. A., Aidyraliev, R. K., Сергиенко, В. И., & IuA, V. (1994) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(94)90137-6 · Cited by 65 · see citing works
Chen, Q., Esterbauer, H., & Jürgens, G. (1992) · Biochemical Journal · doi:10.1042/bj2880249 · Cited by 64 · see citing works
Self-citationHypochlorite induces lipid peroxidation in blood lipoproteins and phospholipid liposomes
Panasenko, O. M., Evgina, S. A., Driomina, E. S., Sharov, V. S., Сергиенко, В. И., & IuA, V. (1995) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(94)00211-2 · Cited by 63 · see citing works
The Respiratory Burst and Its Physiological Significance
Sbarra, A. J., & Strauss, R. R. (1988) · Springer eBooks · doi:10.1007/978-1-4684-5496-3 · Cited by 62 · see citing works
Matheson, N., & Travis, J. W. (1985) · Biochemistry · doi:10.1021/bi00329a021 · Cited by 60 · see citing works
Völker, W. (1997) · Atherosclerosis · doi:10.1016/s0021-9150(96)06039-x · Cited by 57 · see citing works
Human Low Density Lipoprotein as a Target of Hypochlorite Generated by Myeloperoxidase
Jerlich, A., Fabjan, J. S., Tschabuschnig, S., Smirnova, A. V., Horáková, Ľ., Hayn, M., Auer, H., Guttenberger, H., Leis, H., Tatzber, F., et al. (1998) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(97)00439-5 · Cited by 52 · see citing works
Self-citationSepe, S. M., & Clark, R. A. (1985) · The Journal of Immunology · doi:10.4049/jimmunol.134.3.1888 · Cited by 50 · see citing works
The permeability of rabbit leucocytes to sodium, potassium and chloride
Wilson, D. L., & Manery, J. F. (1949) · Journal of Cellular and Comparative Physiology · doi:10.1002/jcp.1030340312 · Cited by 40 · see citing works
Kling, D., Holzschuh, T., & BETZ, E. (1993) · Atherosclerosis · doi:10.1016/0021-9150(93)90104-3 · Cited by 32 · see citing works
Hypochlorite oxidation causes cross-lingking of Lp(a)
O'Connell, A. M., Gieseg, S. P., & Stanley, K. K. (1994) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/0925-4439(94)90076-0 · Cited by 30 · see citing works
Cofactor Role of Iodide in Peroxidase Antimicrobial Action Against Escherichia coli
Thomas, E. L., & Aune, T. M. (1978) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.13.6.1000 · Cited by 29 · see citing works
Jacobsen, D. W. (2000) · Developments in cardiovascular medicine · doi:10.1007/978-94-017-1789-2_3 · Cited by 23 · see citing works
Löffler, G., Petrides, P. E., Weiss, L., & Harper, H. A. (1979) · Springer eBooks · doi:10.1007/978-3-662-09352-8 · Cited by 18 · see citing works
Bergmann, A., Ramos, P., Esterbauer, H., & Winklhofer‐Roob, B. M. (1997) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)30042-0 · Cited by 17 · see citing works
Characteristics of Myeloperoxidase from Neutrophils and Other Peroxidases from Different Cell Types
Zgliczyński, J. M. (1980) · Springer eBooks · doi:10.1007/978-1-4615-9134-4_10 · Cited by 16 · see citing works
Chapter 2. Chemical and Physical Properties of Lipoproteins
Kostner, G. M., & Laggner, P. (1989) · De Gruyter eBooks · doi:10.1515/9783110873665-005 · Cited by 16 · see citing works
The Reticuloendothelial System a Comprehensive Treatise: Biochemistry and Metabolism
Starra, A. J., & Struss, R. R. (1980) · Cited by 12 · see citing works
Serum protein degradation by hypochlorite.
Bp, S., NJu, G., & Sn, L. (1989) · PubMed · Cited by 11 · see citing works
Altered Susceptibility to in Vitro Oxidation of LDL in LDL Complexes and LDL Aggregates
Hermann, M., & Gmeiner, B. M. K. (1993) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1993.tb35734.x · Cited by 10 · see citing works
The Respiratory Burst of Neutrophilic Granulocytes and Its Influence on Infected Tissues
Zgliczyński, J. M., & Stelmaszyńska, T. (1988) · Springer eBooks · doi:10.1007/978-1-4684-5496-3_15 · Cited by 8 · see citing works
A comparative study of serum proteins ability to scavenge active oxygen species: O2-. and OCl-.
Bp, S., NJu, G., & Sn, L. (1988) · PubMed · Cited by 8 · see citing works
Differentiation and Role of Macrophages in the Early Human Atherosclerotic Plaque
Roessner, A., Vollmer, E., Jaeger, E., Rauterberg, J., & Böcker, W. (1993) · Current topics in pathology · doi:10.1007/978-3-642-76849-1_3 · Cited by 6 · see citing works
Recent Progress in Atherosclerosis Research
(1993) · Current topics in pathology · doi:10.1007/978-3-642-76849-1 · Cited by 4 · see citing works
Flagged first, then by citations
1 of the 55 references couldn’t be matched to a known paper.
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Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation
Tejero, J., Shiva, S., & Gladwin, M. T. (2019) · Physiological Reviews · doi:10.1152/physrev.00036.2017 · Cited by 541 · see citing works
Human myeloperoxidase in innate and acquired immunity
Arnhold, J., & Flemmig, J. (2010) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2010.04.008 · Cited by 251 · see citing works
Malle, E., Marsche, G., Arnhold, J., & Davies, M. J. (2006) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2006.03.024 · Cited by 184 · see citing works
Hawkins, C. L., & Davies, M. J. (2021) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2021.07.007 · Cited by 181 · see citing works
The Dual Role of Myeloperoxidase in Immune Response
Arnhold, J. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21218057 · Cited by 161 · see citing works
Lipid analysis of human HDL and LDL by MALDI-TOF mass spectrometry and 31P-NMR
Schiller, J., Zschörnig, O., Petković, M., Müller, M., Arnhold, J., & Arnold, K. (2001) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)34196-1 · Cited by 124 · see citing works
Kinetics and Thermodynamics of Halide and Nitrite Oxidation by Mammalian Heme Peroxidases
Arnhold, J., Monzani, E., Furtmüller, P. G., Zederbauer, M., Casella, L., & Obinger, C. (2006) · European Journal of Inorganic Chemistry · doi:10.1002/ejic.200600436 · Cited by 113 · see citing works
Zhang, C., Yang, J., Jacobs, J. D., & Jennings, L. K. (2003) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00435.2003 · Cited by 86 · see citing works
Schröter, J., & Schiller, J. (2016) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2016/8386362 · Cited by 51 · see citing works
Jerlich, A., Fritz, G., Kharrazi, H., Hammel, M., Tschabuschnig, S., Glatter, O., & Schaur, R. J. (2000) · Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology · doi:10.1016/s0167-4838(00)00112-6 · Cited by 43 · see citing works
Self-citationHalogenation Activity of Mammalian Heme Peroxidases
Arnhold, J., & Malle, E. (2022) · Antioxidants · doi:10.3390/antiox11050890 · Cited by 40 · see citing works
Shariat, S. Z. A. S., Mostafavi, S. A., & Khakpour, F. (1996) · Europe PMC (PubMed Central) · doi:10.6091/ibj.1092.2012 · Cited by 34 · see citing works
Red cabbage anthocyanins may protect blood plasma proteins and lipids
Kołodziejczyk-Czepas, J., Saluk, J., Posmyk, M. M., Janas, K. M., & Wachowicz, B. (2011) · Open Life Sciences · doi:10.2478/s11535-011-0037-5 · Cited by 28 · see citing works
The Role of Halogenative Stress in Atherogenic Modification of Low-Density Lipoproteins
Panasenko, O. M., Torkhovskaya, T. I., Gorudko, I. V., & Av, S. (2020) · Biochemistry (Moscow) · doi:10.1134/s0006297920140035 · Cited by 23 · see citing works
Detection of the halogenating activity of heme peroxidases in leukocytes by aminophenyl fluorescein
Flemmig, J., Remmler, J., Zschaler, J., & Arnhold, J. (2014) · Free Radical Research · doi:10.3109/10715762.2014.999676 · Cited by 21 · see citing works
Panasenko, O. M., Vakhrusheva, T. V., Tretyakov, V., Spalteholz, H., & Arnhold, J. (2007) · Chemistry and Physics of Lipids · doi:10.1016/j.chemphyslip.2007.05.005 · Cited by 20 · see citing works
Kinetics of chlorination of monochlorodimedone by myeloperoxidase
Jerlich, A., Tschabuschnig, S., Fabjan, J. S., & Schaur, R. J. (2000) · International Journal of Clinical & Laboratory Research · doi:10.1007/s005990070031 · Cited by 7 · see citing works
Self-citationRole of Reactive Species in Destructions
Arnhold, J. (2019) · Elsevier eBooks · doi:10.1016/b978-0-12-816388-7.00002-4 · Cited by 5 · see citing works
Jerlich, A., Hammel, M., Horáková, Ľ., & Schaur, R. J. (2001) · Redox Report · doi:10.1179/135100001101536319 · Cited by 2 · see citing works
Self-citation
- Authors
- Jerlich, A., Horakova, L., Fabjan, J. S., Giessauf, A., Schaur, R. J., Jürgens, G., & Schaur, R. J.
- Affiliations
- University of Graz (AT)
- Venue
- International Journal of Clinical & Laboratory Research
- Published
- 1999-12
- Volume
- 29
- Issue
- 4
- Pages
- 155-161
- ISSN
- 0940-5437 (ISSN-L)
- 1434-4467
- OpenAlex
- W2325357782
- Cited by
- 19
- References
- 55
- Citations by year
- 2022: 1 · 2021: 1 · 2020: 2 · 2019: 1 · 2018: 1 · 2016: 1 · 2014: 1
- FWCI
- 0.45
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- International Journal of Clinical & Laboratory Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Life Sciences › Immunology and Microbiology › Immunology › Atherosclerosis and Cardiovascular Diseases
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Keywords
- myeloperoxidase, hypochlorous acid, hydrogen peroxide, chloride, reaction kinetics, atherosclerosis, phagocytes, redox reactions, medium acidity
- MeSH terms
- Adult, Arteriosclerosis, Chlorides, Female, Humans, Hydrogen Peroxide, Hydrogen-Ion Concentration, Hypochlorous Acid, Kinetics, Cholesterol, LDL, Male, Peroxidase, Oxidants, Oxidative Stress
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