Cited by 246Animal Nutrition and PhysiologyGut microbiota and healthRuminant Nutrition and Digestive Physiology

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Utilization of amino acids by bacteria from the pig small intestine

Dai, Zhao-Lai, Zhang, Jing, Wu, Guoyao, Zhu, Wei-Yun·2010·Amino Acids

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Abstract

This study determined the utilization of amino acids (AA) by bacteria from the lumen of the pig small intestine. Digesta samples from different segments of the small intestine were inoculated into media containing 10 mmol/L each of select AA (L-lysine, L-threonine, L-arginine, L-glutamate, L-histidine, L-leucine, L-isoleucine, L-valine, L-proline, L-methionine, L-phenylalanine or L-tryptophan) and incubated for 24 h. The previous 24-h culture served as an inoculum for a subsequent 24-h subculture during each of 30 subcultures. Results of the in vitro cultivation experiment indicated that the 24-h disappearance rates for lysine, arginine, threonine, glutamate, leucine, isoleucine, valine or histidine were 50-90% in the duodenum, jejunum or ileum groups. After 30 subcultures, the 24-h disappearance rates for lysine, threonine, arginine or glutamate remained greater than 50%. The denaturing gradient gel electrophoresis analysis showed that Streptococcus sp., Mitsuokella sp., and Megasphaera elsdenii-like bacteria were predominant in subcultures for utilizing lysine, threonine, arginine and glutamate. In contrast, Klebsiella sp. was not a major user of arginine or glutamate. Furthermore, analysis of AA composition and the incorporation of AA into polypeptides indicated that protein synthesis was a major pathway for AA metabolism in all the bacteria studied. The current work identified the possible predominant bacterial species responsible for AA metabolism in the pig small intestine. The findings provide a new framework for future studies to characterize the metabolic fate of AA in intestinal microbes and define their nutritional significance for both animals and humans.

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Authors: Dai, Z.-L., Zhang, J., Wu, G., & Zhu, W.-Y.
Affiliations: Nanjing Agricultural University (CN)
Texas A&M University (US)
ORCID: Zhang, Jing
Wu, Guoyao
Venue: Amino Acids
Publisher: Springer Science and Business Media LLC
Published: 2010-03-19
Volume: 39
Issue: 5
Pages: 1201-1215
ISSN: 0939-4451 (ISSN-L)
1438-2199
DOI: 10.1007/s00726-010-0556-9
OpenAlex: W2021934488
Cited by: 246
References: 73
Citations by year: 2026: 11 · 2025: 15 · 2024: 17 · 2023: 29 · 2022: 30 · 2021: 31 · 2020: 16 · 2019: 15 · 2018: 15 · 2017: 13 · 2016: 7 · 2015: 8 · 2014: 9 · 2013: 11 · 2012: 8
FWCI: 16.08
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Amino Acids (publishedVersion)
PubMed (publishedVersion)
Funders: Texas AgriLife Research
National Key Research and Development Program of China
Topics: Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Ruminant Nutrition and Digestive Physiology
Keywords: amino acid utilization, intestinal bacteria, pig small intestine, amino acid metabolism, lysine, L-arginine, glutamate, threonine, protein synthesis, Streptococcus, Mitsuokella, Megasphaera elsdenii, Klebsiella, bacterial community composition
MeSH terms: Amino Acids, Animals, Bacteria, Intestine, Small, Swine, Denaturing Gradient Gel Electrophoresis

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Utilization of amino acids by bacteria from the pig small intestine

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