The DOI matches its Crossref record; 74 of the 74 references it cites were found in a registry or an index.
Utilization of amino acids by bacteria from the pig small intestine
Abstract
This study determined the utilization of amino acids (AA) by bacteria from the lumen of the pig small intestine. Digesta samples from different segments of the small intestine were inoculated into media containing 10 mmol/L each of select AA (L-lysine, L-threonine, L-arginine, L-glutamate, L-histidine, L-leucine, L-isoleucine, L-valine, L-proline, L-methionine, L-phenylalanine or L-tryptophan) and incubated for 24 h. The previous 24-h culture served as an inoculum for a subsequent 24-h subculture during each of 30 subcultures. Results of the in vitro cultivation experiment indicated that the 24-h disappearance rates for lysine, arginine, threonine, glutamate, leucine, isoleucine, valine or histidine were 50-90% in the duodenum, jejunum or ileum groups. After 30 subcultures, the 24-h disappearance rates for lysine, threonine, arginine or glutamate remained greater than 50%. The denaturing gradient gel electrophoresis analysis showed that Streptococcus sp., Mitsuokella sp., and Megasphaera elsdenii-like bacteria were predominant in subcultures for utilizing lysine, threonine, arginine and glutamate. In contrast, Klebsiella sp. was not a major user of arginine or glutamate. Furthermore, analysis of AA composition and the incorporation of AA into polypeptides indicated that protein synthesis was a major pathway for AA metabolism in all the bacteria studied. The current work identified the possible predominant bacterial species responsible for AA metabolism in the pig small intestine. The findings provide a new framework for future studies to characterize the metabolic fate of AA in intestinal microbes and define their nutritional significance for both animals and humans.
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“Additionally, BCAAs can be utilized by microbes, potentially regulating intestinal microbial species, diversity, and metabolism (333, 334).”
From Signalling cognition: The gut microbiota and hypothalamic-pituitary-adrenal axis (2023) · cites this paper as [333]
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Self-citationAmino acids and immune function
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Self-citationArginine metabolism and nutrition in growth, health and disease
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Research works printed but not linked: 1
- Zhang J (2009) Isolation and identification of amino acid utilizing bacteria from the porcine small intestine. Thesis, Nanjing Agricultural University, Nanjing, China
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Rowland, I., Gibson, G., Heinken, A., Scott, K., Swann, J., Thiele, I., & Tuohy, K. (2018) · European Journal of Nutrition · doi:10.1007/s00394-017-1445-8 · Cited by 2,878
The role of microbial amino acid metabolism in host metabolism
Neis, E., Dejong, C., & Rensen, S. (2015) · Nutrients · doi:10.3390/nu7042930 · Cited by 920
Functional Amino Acids in Growth, Reproduction, and Health
Wu, G. (2010) · Advances in Nutrition · doi:10.3945/an.110.1008 · Cited by 791
Self-citationGlycine metabolism in animals and humans: implications for nutrition and health
Wang, W., Wu, Z., Dai, Z., Yang, Y., Wang, J., & Wu, G. (2013) · Amino Acids · doi:10.1007/s00726-013-1493-1 · Cited by 766
Self-citationProline and hydroxyproline metabolism: implications for animal and human nutrition
Wu, G., Bazer, F. W., Burghardt, R. C., Johnson, G. A., Kim, S. W., Knabe, D. A., Li, P., Li, X., McKnight, J. R., Satterfield, M. C., et al. (2010) · Amino Acids · doi:10.1007/s00726-010-0715-z · Cited by 707
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Metadata
- Authors
- Dai, Z.-L., Zhang, J., Wu, G., & Zhu, W.-Y.
- Affiliations
- Nanjing Agricultural University (CN)
- Texas A&M University (US)
- Venue
- Amino Acids
- Published
- 2010-03-19
- Volume
- 39
- Issue
- 5
- Pages
- 1201-1215
- ISSN
- 0939-4451 (ISSN-L)
- 1438-2199
- OpenAlex
- W2021934488
- Cited by
- 246
- References
- 73
- Citations by year
- 2026: 11 · 2025: 15 · 2024: 17 · 2023: 29 · 2022: 30 · 2021: 31 · 2020: 16 · 2019: 15 · 2018: 15 · 2017: 13 · 2016: 7 · 2015: 8 · 2014: 9 · 2013: 11 · 2012: 8
- FWCI
- 16.08
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Amino Acids (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Texas AgriLife Research
- National Key Research and Development Program of China
- Topics
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Ruminant Nutrition and Digestive Physiology
- Keywords
- amino acid utilization, intestinal bacteria, pig small intestine, amino acid metabolism, lysine, L-arginine, glutamate, threonine, protein synthesis, Streptococcus, Mitsuokella, Megasphaera elsdenii, Klebsiella, bacterial community composition
- MeSH terms
- Amino Acids, Animals, Bacteria, Intestine, Small, Swine, Denaturing Gradient Gel Electrophoresis
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