Solute accumulation in the deep-sea bacterium Photobacterium profundum
Abstract
The identity and amounts of intracellular solutes in the deep-sea bacterium Photobacterium profundum strain SS9 were studied using nuclear magnetic resonance techniques. P. profundum strain SS9, a moderate piezophile which grows optimally at 20-30 MPa primarily accumulated glutamate and betaine, with lesser amounts of alanine, beta-hydroxybutyrate (beta-HB) and oligomers composed of the beta-HB units when grown at 0.1 MPa to early stationary phase. When grown at the optimal pressure, the cells preferentially increased intracellular concentrations of beta-HB and beta-HB oligomers, while the amino acid pools remained relatively constant. Since the organic solutes increased with increasing external NaCl in the medium, they are functioning as osmolytes. The beta-HB molecules represent a novel class of osmolytes, termed 'piezolytes,' whose cellular levels responded to hydrostatic pressure as well as osmotic pressure. Factors such as cell growth stage and temperature were also examined for their effect on the solute distribution in these cells.
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Piezolytes are small organic solutes that counteract pressure effects on proteins, potentially allowing proteins to work over greater depth ranges but possibly constraining species depths if their regulation is limited.
“Recently, another mechanism for pressure adaptation has been hypothesized involving “piezolytes” (11,15): small organic solutes that counteract the effects of pressure on proteins, potentially allowing proteins to work over greater depth ranges but possibly also constraining species depths if regulation of these solutes is limited (11,(13)(14)(15)(16)(17).”
From Marine fish may be biochemically constrained from inhabiting the deepest ocean depths (2014) · cites this paper as [15]
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- Authors
- Martin, D., Bartlett, D., & Roberts, M.
- Affiliations
- Boston College (US)
- Scripps Institution of Oceanography (US)
- Corresponding author
- Bartlett, Douglas
- Venue
- Extremophiles
- Published
- 2002-12-01
- Volume
- 6
- Issue
- 6
- Pages
- 507-514
- ISSN
- 1431-0651 (ISSN-L)
- 1433-4909
- OpenAlex
- W2026086591
- Cited by
- 133
- References
- 51
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 5 · 2023: 8 · 2022: 10 · 2021: 10 · 2020: 14 · 2019: 5 · 2018: 9 · 2017: 9 · 2016: 6 · 2015: 9 · 2014: 6 · 2013: 6 · 2012: 5
- FWCI
- 1.95
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Extremophiles (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Physical Sciences › Environmental Science › Ecology › Polar Research and Ecology
- Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
- Keywords
- Photobacterium profundum, osmolytes, piezophiles, hydrostatic pressure, glutamate, betaine, beta-hydroxybutyrate, amino acid pool, sodium chloride, growth stages, temperature effects, deep-sea bacteria
- MeSH terms
- Adaptation, Physiological, Alanine, Betaine, Biological Transport, Hydrostatic Pressure, Intracellular Fluid, Models, Biological, Photobacterium, Saline Solution, Hypertonic, Temperature, Water Microbiology, Water-Electrolyte Balance, Glutamic Acid, Nuclear Magnetic Resonance, Biomolecular, 3-Hydroxybutyric Acid
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