Irradiation by high-intensity red light-emitting diode enhances human bone marrow mesenchymal stem cells osteogenic differentiation and mineralization through Wnt/β-catenin signaling pathway
Abstract
Photobiomodulation therapy (PBMT) using a light-emitting diode (LED) has been employed for various photomedicine studies. The aim of this study was to determine the effects of a high-intensity red LED on the proliferation and osteogenic differentiation of human bone marrow mesenchymal stem cells (BMSCs) and the related mechanism. BMSCs were subjected to high-intensity red LED (LZ1-00R205 Deep Red LED) irradiations for 0 to 40 s with energy densities ranging from 0 to 8 J/cm 2. The distance from the LED to the cell layer was 40 mm. The spot size on the target was 4 cm 2. Cell proliferation was measured at 3, 24, 48, and 72 h. The effects of LED irradiation on osteogenic differentiation and mineralization were examined with a particular focus on the Wnt/β-catenin signaling pathway. The high-intensity red LED irradiations did not alter BMSC proliferation after 72 h. LED exposure of 6 J/cm 2 (30 s) led to significant enhancements of osteogenic differentiation and mineralization. Additionally, the high-intensity LED irradiation induced activation of Wnt/β-catenin. The effects of the high-intensity LED irradiation on BMSC osteogenic differentiation and mineralization were suppressed by treatment with the Wnt/β-catenin inhibitor XAV939. P < 0.05 was considered significant. The results indicate that high-intensity red LED irradiation increases BMSC osteogenic differentiation and mineralization via Wnt/β-catenin activation. Therefore, short duration irradiation with a portable high-intensity LED may be used as a potential approach in hard tissue regeneration therapy.
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The Wnt/β-catenin pathway enhances osteogenic differentiation of mesenchymal stem cells, bone formation and mineralization.
“Apart from suppressing osteoclastogenesis, it also enhances the osteogenic differentiation of MSCs [97], bone formation [98] and mineralization [99].”
From An Overlooked Bone Metabolic Disorder: Cigarette Smoking-Induced Osteoporosis (2022) · cites this paper as [99]
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An Overlooked Bone Metabolic Disorder: Cigarette Smoking-Induced Osteoporosis
Weng, W., Li, H., & Zhu, S. (2022) · Genes · doi:10.3390/genes13050806 · Cited by 65 · see citing works
- Authors
- Ruan, Y., Kato, H., Taguchi, Y., Yamauchi, N., & Umeda, M.
- Affiliations
- Osaka Dental University (JP)
- Corresponding author
- Taguchi, Yoichiro
- Venue
- Lasers in Medical Science
- Published
- 2020-06-25
- Volume
- 36
- Issue
- 1
- Pages
- 55-65
- ISSN
- 0268-8921 (ISSN-L)
- 1435-604X
- OpenAlex
- W3037071505
- Cited by
- 37
- References
- 54
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 10 · 2023: 4 · 2022: 6 · 2021: 7 · 2020: 1
- FWCI
- 2.20
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Lasers in Medical Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Laser Applications in Dentistry and Medicine
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Health Sciences › Medicine › Physiology › Biofield Effects and Biophysics
- Keywords
- low-level laser therapy, red light-emitting diodes, bone marrow mesenchymal stem cells, osteogenic differentiation, mineralization, Wnt/β-catenin signaling, XAV939, cell proliferation, hard tissue regeneration
- MeSH terms
- Calcification, Physiologic, Cell Differentiation, Cells, Cultured, Humans, Light, Osteogenesis, Cell Proliferation, Mesenchymal Stem Cells, Wnt Signaling Pathway
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