Cited by 37Laser Applications in Dentistry and MedicineMesenchymal stem cell researchBiofield Effects and Biophysics

Irradiation by high-intensity red light-emitting diode enhances human bone marrow mesenchymal stem cells osteogenic differentiation and mineralization through Wnt/β-catenin signaling pathway

Ruan, Yaru, Kato, Hirohito, Taguchi, Yoichiro, Yamauchi, Nobuhiro, Umeda, Makoto·2021·Lasers in Medical Science

Abstract

Photobiomodulation therapy (PBMT) using a light-emitting diode (LED) has been employed for various photomedicine studies. The aim of this study was to determine the effects of a high-intensity red LED on the proliferation and osteogenic differentiation of human bone marrow mesenchymal stem cells (BMSCs) and the related mechanism. BMSCs were subjected to high-intensity red LED (LZ1-00R205 Deep Red LED) irradiations for 0 to 40 s with energy densities ranging from 0 to 8 J/cm 2. The distance from the LED to the cell layer was 40 mm. The spot size on the target was 4 cm 2. Cell proliferation was measured at 3, 24, 48, and 72 h. The effects of LED irradiation on osteogenic differentiation and mineralization were examined with a particular focus on the Wnt/β-catenin signaling pathway. The high-intensity red LED irradiations did not alter BMSC proliferation after 72 h. LED exposure of 6 J/cm 2 (30 s) led to significant enhancements of osteogenic differentiation and mineralization. Additionally, the high-intensity LED irradiation induced activation of Wnt/β-catenin. The effects of the high-intensity LED irradiation on BMSC osteogenic differentiation and mineralization were suppressed by treatment with the Wnt/β-catenin inhibitor XAV939. P < 0.05 was considered significant. The results indicate that high-intensity red LED irradiation increases BMSC osteogenic differentiation and mineralization via Wnt/β-catenin activation. Therefore, short duration irradiation with a portable high-intensity LED may be used as a potential approach in hard tissue regeneration therapy.

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Crossref · 10.1007/s10103-020-03002-5

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54 of 54 references verified0 citation errors · 0 not checkable

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  • The Wnt/β-catenin pathway enhances osteogenic differentiation of mesenchymal stem cells, bone formation and mineralization.

    “Apart from suppressing osteoclastogenesis, it also enhances the osteogenic differentiation of MSCs [97], bone formation [98] and mineralization [99].”

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Authors
Ruan, Y., Kato, H., Taguchi, Y., Yamauchi, N., & Umeda, M.
Affiliations
Osaka Dental University (JP)
Corresponding author
Taguchi, Yoichiro
Venue
Lasers in Medical Science
Published
2020-06-25
Volume
36
Issue
1
Pages
55-65
ISSN
  • 0268-8921 (ISSN-L)
  • 1435-604X
OpenAlex
W3037071505
Cited by
37
References
54
Citations by year
2026: 4 · 2025: 5 · 2024: 10 · 2023: 4 · 2022: 6 · 2021: 7 · 2020: 1
FWCI
2.20
Type
journal article
Language
en
Open access
closed
Locations
  • Lasers in Medical Science (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Laser Applications in Dentistry and Medicine
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
  • Health Sciences › Medicine › Physiology › Biofield Effects and Biophysics
Keywords
low-level laser therapy, red light-emitting diodes, bone marrow mesenchymal stem cells, osteogenic differentiation, mineralization, Wnt/β-catenin signaling, XAV939, cell proliferation, hard tissue regeneration
MeSH terms
Calcification, Physiologic, Cell Differentiation, Cells, Cultured, Humans, Light, Osteogenesis, Cell Proliferation, Mesenchymal Stem Cells, Wnt Signaling Pathway

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