Kefiran Protects Caco-2 Cells from Cytopathic Effects Induced by Bacillus cereus Infection
Open accessAbstract
The aim of this work was to evaluate the ability of kefiran to antagonize cytopathic effects triggered by Bacillus cereus strain B10502 on cultured human enterocytes (Caco-2 cells). Cell damage was evaluated by F-actin labelling, scanning electron microscopy and determination of ratios of necrotic and detached cells. To assess the interaction between kefiran and bacteria or eukaryotic cells, flow cytometric analysis was conducted with FITC-labelled kefiran. Kefiran significantly protected infected cells from cytopathic effects induced by B. cereus such as cell necrosis, F-actin disorganisation and microvilli effacement, although presence of kefiran did not modify the adhesion of microorganisms to cultured human enterocytes. Results could be ascribed to the ability of kefiran to interact with both bacteria and eukaryotic cells thus antagonizing interactions necessary for maximal biological effects. Our findings encourage further research on the use of bacterial exopolysaccharides to antagonize virulence factors associated to direct bacteria-cell interactions.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Lb. kefiranofaciens is the best producer of kefiran, a water-soluble EPS produced in kefir grains; kefiran is a branched glucogalactan composed of hexa- or heptasaccharide repeating structure with almost equal amounts of glucose and galactose.
“Lb. kefiranofaciens is the best producer of kefiran, a water-soluble EPS produced in kefir grains. It is a branched glucogalactan composed of hexa- or heptasaccharide repeating structure with almost equal amounts of glucose and galactose [180].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [180]
Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302 · see citing works
Biological roles of oligosaccharides: all of the theories are correct
Varki, A. P. (1993) · Glycobiology · doi:10.1093/glycob/3.2.97 · Cited by 5,203 · see citing works
Food microbiology : fundamentals and frontiers
Doyle, M. P., Beuchat, L. R., & Montville, T. J. (2013) · doi:10.1128/9781555819972 · Cited by 3,589 · see citing works
Pinto, M. (1983) · HAL · Cited by 1,752 · see citing works
One Hundred and Twenty-Seven Cultured Human Tumor Cell Lines Producing Tumors in Nude Mice23
Fogh, J., Fogh, J. M., & Orfeo, T. (1977) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/59.1.221 · Cited by 1,599 · see citing works
Hakomori, S. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)30565-3 · Cited by 878 · see citing works
Bacillus cereus and related species
Drobniewski, F. A. (1993) · Clinical Microbiology Reviews · doi:10.1128/cmr.6.4.324 · Cited by 836 · see citing works
Epidemiology and pathogenesis of infections
Kotiranta, A. K., Lounatmaa, K., & Haapasalo, M. (2000) · Microbes and Infection · doi:10.1016/s1286-4579(00)00269-0 · Cited by 646 · see citing works
INFECTIONS OF THE GASTRO-INTESTINAL TRACT
(1980) · Elsevier eBooks · doi:10.1016/b978-0-433-30301-5.50017-5 · Cited by 619 · see citing works
A new cytotoxin from Bacillus cereus that may cause necrotic enteritis
Lund, T., Buyser, M. D., & Granum, P. E. (2000) · Molecular Microbiology · doi:10.1046/j.1365-2958.2000.02147.x · Cited by 591 · see citing works
Infections of the Gastrointestinal Tract
Wong, E. S. (1996) · JAMA · doi:10.1001/jama.1996.03530280083052 · Cited by 515 · see citing works
Ganglioside-mediated modulation of cell growth, growth factor binding, and receptor phosphorylation.
Bremer, E., Hakomori, S., Bowen‐Pope, D. F., Raines, E. W., & Ross, R. (1984) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)39801-0 · Cited by 498 · see citing works
Crystallographic structure of an intact IgG1 monoclonal antibody 1 1Edited by I. A. Wilson
Harris, L. J., Skaletsky, E., & McPherson, A. (1998) · Journal of Molecular Biology · doi:10.1006/jmbi.1997.1508 · Cited by 440 · see citing works
Chemical and microbiological characterisation of kefir grains
GARROTE, G. L., ABRAHAM, A. G., & DE ANTONI, G. L. (2001) · Journal of Dairy Research · doi:10.1017/s0022029901005210 · Cited by 332 · see citing works
Self-citationBacterial Protein Toxins: An Overview
Alouf, J. E. (2003) · Humana Press eBooks · doi:10.1385/1-59259-052-7:1 · Cited by 329 · see citing works
A novel dodecadepsipeptide, cereulide, is an emetic toxin of
Agata, N., Ohta, M., MORI, M., & Isobe, M. (1995) · FEMS Microbiology Letters · doi:10.1016/0378-1097(95)00119-p · Cited by 316 · see citing works
Enterotoxic activity of hemolysin BL from Bacillus cereus
Beecher, D. J., Schoeni, J. L., & Wong, A. C. L. (1995) · Infection and Immunity · doi:10.1128/iai.63.11.4423-4428.1995 · Cited by 315 · see citing works
Vinderola, G., Perdigón, G., Duarte, J., Farnworth, E. R., & Matar, C. (2006) · Cytokine · doi:10.1016/j.cyto.2007.01.003 · Cited by 291 · see citing works
Agata, N., Mori, M., Ohta, M., et al. (1994) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1994.tb07071.x · Cited by 253 · see citing works
Ruas‐Madiedo, P., Gueimonde, M., Margollés, A., Reyes‐Gavilán, C. G. D. L., & Salminen, S. (2006) · Journal of Food Protection · doi:10.4315/0362-028x-69.8.2011 · Cited by 241 · see citing works
Role of Oligosaccharide Residues of IgG1-Fc in FcγRIIb Binding
Mimura, Y., Sondermann, P., Ghirlando, R., Lund, J., Young, S. P., Goodall, M., & Jefferis, R. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m107478200 · Cited by 236 · see citing works
Gangliosides as modulators of cell function
Zeller, C. B., & Marchase, R. B. (1992) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1992.262.6.c1341 · Cited by 208 · see citing works
Adhesion of bacillus spores in relation to hydrophobicity
Rönner, U., Husmark, U., & Henriksson, A. (1990) · Journal of Applied Bacteriology · doi:10.1111/j.1365-2672.1990.tb01547.x · Cited by 194 · see citing works
Beecher, D. J., & Wong, A. C. L. (1994) · Applied and Environmental Microbiology · doi:10.1128/aem.60.5.1646-1651.1994 · Cited by 166 · see citing works
Lund, T., & Granum, P. E. (1997) · Microbiology · doi:10.1099/00221287-143-10-3329 · Cited by 162 · see citing works
Carbohydrate-mediated recognition systems in innate immunity
Feizi, T. (2000) · Immunological Reviews · doi:10.1034/j.1600-065x.2000.917310.x · Cited by 161 · see citing works
Gelling properties of kefiran, a food-grade polysaccharide obtained from kefir grain
Piermaría, J., de la Canal, M. L., & Abraham, A. G. (2007) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2007.10.005 · Cited by 143 · see citing works
Self-citationMcKillip, J. L. (2000) · Antonie van Leeuwenhoek · doi:10.1023/a:1002706906154 · Cited by 140 · see citing works
Ghelardi, E., Celandroni, F., Salvetti, S., et al. (2002) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2002.tb11072.x · Cited by 139 · see citing works
Micheli, L., Uccelletti, D., Palleschi, C., & Crescenzi, V. (1999) · Applied Microbiology and Biotechnology · doi:10.1007/s002530051616 · Cited by 137 · see citing works
Andersson, A., Granum, P. E., & Rönner, U. (1998) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(97)00121-9 · Cited by 123 · see citing works
Beecher, D. J., Pulido, J. S., Barney, N. P., et al. (1995) · Infection and Immunity · doi:10.1128/iai.63.2.632-639.1995 · Cited by 119 · see citing works
Beecher, D. J., Olsen, T. W., Somers, E. B., et al. (2000) · Infection and Immunity · doi:10.1128/iai.68.9.5269-5276.2000 · Cited by 116 · see citing works
Callegan, M. C., Jett, B. D., Hancock, L. E., et al. (1999) · Infection and Immunity · doi:10.1128/iai.67.7.3357-3366.1999 · Cited by 113 · see citing works
Kefiran antagonizes cytopathic effects of Bacillus cereus extracellular factors
Medrano, M., Pérez, P. F., & Abraham, A. G. (2007) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2007.11.046 · Cited by 107 · see citing works
Self-citationRamarao, N., & Lereclus, D. (2006) · Microbes and Infection · doi:10.1016/j.micinf.2006.01.005 · Cited by 104 · see citing works
Hardy, S. P., Lund, T., & Granum, P. E. (2001) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2001.tb10581.x · Cited by 104 · see citing works
Polysaccharide production by kefir grains during whey fermentation
Rimada, P. S., & Abraham, A. G. (2001) · Journal of Dairy Research · doi:10.1017/s0022029901005131 · Cited by 89 · see citing works
Self-citationThe contribution of gC1qR/p33 in infection and inflammation
Peerschke, E. I. B., & Ghebrehiwet, B. (2007) · Immunobiology · doi:10.1016/j.imbio.2006.11.011 · Cited by 85 · see citing works
Guignot, J., Bernet‐Camard, M., Poüs, C., Plançon, L., Bouguénec, C. L., & Servin, A. L. (2001) · Infection and Immunity · doi:10.1128/iai.69.3.1856-1868.2001 · Cited by 78 · see citing works
(2022) · Cellular Microbiology · doi:10.1155/cmi · Cited by 78 · see citing works
Beecher, D. J., & Wong, A. C. L. (2000) · Microbiology · doi:10.1099/00221287-146-6-1371 · Cited by 77 · see citing works
Characterization of kefir grains grown in cows' milk and in soya milk
Abraham, A. G., & Antoni, G. L. D. (1999) · Journal of Dairy Research · doi:10.1017/s0022029999003490 · Cited by 63 · see citing works
Self-citationEffect of Bacillus cereus Exocellular Factors on Human Intestinal Epithelial Cells
Minnaard, J., Humen, M. A., & Pérez, P. F. (2001) · Journal of Food Protection · doi:10.4315/0362-028x-64.10.1535 · Cited by 48 · see citing works
Self-citationCellular Microbiology: Bacteria-Host Interactions in Health and Disease
Henderson, B. E. (1999) · UCL Discovery (University College London) · Cited by 44 · see citing works
Minnaard, J., Moal, V. L.-L., Coconnier, M. H., et al. (2004) · Infection and Immunity · doi:10.1128/iai.72.6.3106-3112.2004 · Cited by 44 · see citing works
Mikami, T., Horikawa, T., Murakami, T., et al. (1994) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1994.tb06866.x · Cited by 39 · see citing works
Virulence of Bacillus cereus: A multivariate analysis
Minnaard, J., Delfederico, L., Vasseur, V., Hollmann, A., Rolny, I. S., Semorile, L., & Pérez, P. F. (2007) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2006.12.013 · Cited by 38 · see citing works
Self-citationInteraction of a rat lung lectin with the exopolysaccharides of Pseudomonas aeruginosa
McArthur, H. A. I., & Ceri, H. (1983) · Infection and Immunity · doi:10.1128/iai.42.2.574-578.1983 · Cited by 30 · see citing works
Ghebrehiwet, B., Tantral, L., Titmus, M. A., et al. (2007) · Springer eBooks · doi:10.1007/978-0-387-71767-8_13 · Cited by 25 · see citing works
Sphingomyelinase is part of the ‘enterotoxin complex’ produced byBacillus cereus
Granum, P. E., & Nissen, H. (1993) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1993.tb06301.x · Cited by 25 · see citing works
Glycoconjugates for the recognition of Bacillus spores
Tarasenko, O., Islam, S., Paquiot, D., et al. (2004) · Carbohydrate Research · doi:10.1016/j.carres.2004.10.008 · Cited by 19 · see citing works
Bacterial Adherence to Cell Surfaces and Extracellular Matrix
Finlay, B. B., & Caparon, M. G. (2014) · ASM Press eBooks · doi:10.1128/9781555817633.ch5 · Cited by 15 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Lynch, K. M., Zannini, E., Coffey, A., & Arendt, E. K. (2018) · Annual Review of Food Science and Technology · doi:10.1146/annurev-food-030117-012537 · Cited by 289 · see citing works
Exopolysaccharides as Antimicrobial Agents: Mechanism and Spectrum of Activity
Abdalla, A. K., Ayyash, M. M., Olaimat, A. N., Osaili, T. M., Al-Nabulsi, A. A., Shah, N. P., & Holley, R. (2021) · Frontiers in Microbiology · doi:10.3389/fmicb.2021.664395 · Cited by 268 · see citing works
Kefir and Health: A Contemporary Perspective
Ahmed, Z., Wang, Y., Ahmad, A., Khan, S. T., Nisa, M., Ahmad, H., & Afreen, A. (2013) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2010.540360 · Cited by 242 · see citing works
Biopolymers from Lactic Acid Bacteria. Novel Applications in Foods and Beverages
Torino, M. I., Font de Valdez, G., & Mozzi, F. (2015) · Frontiers in Microbiology · doi:10.3389/fmicb.2015.00834 · Cited by 201 · see citing works
Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods
Prete, R., Alam, M. K., Perpetuini, G., Perla, C., Pittia, P., & Corsetti, A. (2021) · Foods · doi:10.3390/foods10071653 · Cited by 194 · see citing works
A review: chemical, microbiological and nutritional characteristics of kefir
Arslan, S. (2015) · CyTA - Journal of Food · doi:10.1080/19476337.2014.981588 · Cited by 160 · see citing works
- Authors
- Medrano, M., Hamet, M. F., Abraham, A. G., & Pérez, P. F.
- Affiliations
- Consejo Nacional de Investigaciones Científicas y Técnicas (AR)
- Corresponding author
- Pérez, Pablo F.
- Venue
- Antonie van Leeuwenhoek
- Published
- 2009-07-25
- Volume
- 96
- Issue
- 4
- Pages
- 505-513
- ISSN
- 0003-6072 (ISSN-L)
- 1572-9699
- OpenAlex
- W2006995028
- Cited by
- 46
- References
- 53
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 4 · 2023: 2 · 2022: 3 · 2021: 6 · 2020: 4 · 2019: 1 · 2018: 3 · 2017: 1 · 2016: 3 · 2015: 3 · 2014: 4 · 2013: 2 · 2012: 1
- FWCI
- 3.71
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- cc-by-nc-sa
- www.springer.com/tdm (tdm)
- Locations
- Antonie van Leeuwenhoek (publishedVersion)
- PubMed (publishedVersion)
- Conicet (submittedVersion, cc-by-nc-sa)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacillus and Francisella bacterial research
- Keywords
- kefiran, Bacillus cereus, Caco-2 cells, enterocytes, cytopathic effect, bacterial exopolysaccharides, necrosis, bacterial adhesion, probiotic protection, virulence factors
- MeSH terms
- Actins, Anti-Bacterial Agents, Bacillus cereus, Bacterial Adhesion, Cell Survival, Flow Cytometry, Humans, Microscopy, Electron, Scanning, Polysaccharides, Staining and Labeling, Cell Death, Caco-2 Cells
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
