Using redox potential to detect microbial activities during clavulanic acid biosynthesis in Streptomyces clavuligerus
Abstract
Production of clavulanic acid (CA) by Streptomyces clavuligerus in a shake-flask culture increased from 92 to 180 mg l(-1) with an increased O2 transfer efficiency (0.039 --> 0.058 s(-1)), which maintained the redox potential values above -250 mV. Compared with traditional measures, such as dissolved O2 concentration and respiratory activity, the redox potential can easily be determined and correlates closely with CA production. It can therefore be used to monitor microbial activities during biosyntheses of secondary metabolites.
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Lin et al. (2005) found that a flask with higher baffle height had a slight increase in clavulanic acid production (180 mg/L) compared to a normal Erlenmeyer flask (90 mg/L).
“The results showed that the flask with a higher baffle height had a slight increase in the production of clavulanic acid (180 mg/L), as compared to a normal Erlenmeyer flask (90 mg/L).”
From Fermentation Conditions that Affect Clavulanic Acid Production in Streptomyces clavuligerus: A Systematic Review (2016) · cites this paper as Lin et al., 2005
Cites
Naturally-occurring beta-lactamase inhibitors with antibacterial activity
BROWN, A. G., BUTTERWORTH, D., COLE, M., HANSCOMB, G., HOOD, J. D., READING, C., & ROLINSON, G. N. (1976) · The Journal of Antibiotics · doi:10.7164/antibiotics.29.668 · Cited by 399 · see citing works
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Ser, H.-L., Law, J. W.-F., Chaiyakunapruk, N., Jacob, S. A., Palanisamy, U. D., Chan, K.-G., Goh, B.-H., & Lee, L.-H. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00522 · Cited by 90 · see citing works
“In fact, the effect of aeration on the production of clavulanic acid can be tested with a direct experiment involving the use of the Erlenmeyer flask with different baffle heights (Lin et al., 2005).”
Method
- Authors
- Lin, Y.-H., Hwang, S.-C. J., Gong, J. T., Wu, J.-Y., & Chen, K.-C.
- Affiliations
- Industrial Technology Research Institute (TW)
- Chung Hua University (TW)
- National Tsing Hua University (TW)
- Dayeh University (TW)
- Corresponding author
- Hwang, Sz-Chwun John
- ORCID
- Wu, Jane-Yii
- Venue
- Biotechnology Letters
- Published
- 2005-11
- Volume
- 27
- Issue
- 22
- Pages
- 1791-1795
- ISSN
- 0141-5492 (ISSN-L)
- 1573-6776
- OpenAlex
- W2090482640
- Cited by
- 7
- References
- 1
- Citations by year
- 2025: 1 · 2021: 1 · 2019: 1 · 2016: 2
- FWCI
- 0.42
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Biotechnology Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
- Physical Sciences › Engineering › Biomedical Engineering › Fluid Dynamics and Mixing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- clavulanic acid, Streptomyces clavuligerus, redox potential, microbial activity monitoring, oxygen transfer efficiency, secondary metabolite biosynthesis, dissolved oxygen, respiratory activity, shake flask culture
- MeSH terms
- Oxidation-Reduction, Oxygen, Oxygen Consumption, Polarography, Streptomyces, Cell Culture Techniques, Bioreactors, Clavulanic Acid
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