Chemical and biological characterization of siderophore produced by the marine-derived Aureobasidium pullulans HN6.2 and its antibacterial activity
Abstract
After analysis using HPLC and electronic ion spray mass spectroscopy, the purified siderophore produced by the marine-derived Aureobasidium pullulans HN6.2 was found to be fusigen. The purified desferric fusigen still had strong inhibition of growth of the pathogenic Vibrio anguillarum while the fusigen chelated by Fe3+ lost the ability to inhibit the growth of the pathogenic bacterium. The added iron in the medium repressed expression of the hydroxylase gene encoding ornithine N5-oxygenase that catalyzes the N5-hydroxylation of ornithine for the first step of siderophore biosynthesis in the yeast cells while expression of the hydroxylase gene in the yeast cells grown in the medium plus ornithine was enhanced.
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In Aureobasidium pullulans, a siderophore identified as fusarinine C (fusigen) was identified and shown to exhibit antibacterial activity.
“In Aureobasidium pullulans, a siderophore identified as fusarinine C (fusigen) was identified and shown to exhibit antibacterial activity (Wang et al. 2009a, b).”
From Biocontrol yeasts: mechanisms and applications (2019) · cites this paper as Wang et al. 2009a, b
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Self-citation
- Authors
- Wang, W., Chi, Z., Liu, G., Buzdar, M. A., Chi, Z., & Gu, Q.
- Affiliations
- Ocean University of China (CN)
- Venue
- BioMetals
- Published
- 2009-05-21
- Volume
- 22
- Issue
- 6
- Pages
- 965-972
- ISSN
- 0966-0844 (ISSN-L)
- 1572-8773
- OpenAlex
- W2067429517
- Cited by
- 53
- References
- 25
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 1 · 2023: 3 · 2022: 4 · 2021: 7 · 2020: 3 · 2019: 3 · 2018: 4 · 2017: 2 · 2016: 4 · 2015: 3 · 2014: 2 · 2013: 2 · 2012: 1
- FWCI
- 1.92
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- BioMetals (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China
- Topics
- Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Metabolism and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Marine Sponges and Natural Products
- Keywords
- siderophores, Aureobasidium pullulans, Vibrio anguillarum, antibacterial activity, iron chelation, ornithine, siderophore biosynthesis, marine fungi
- MeSH terms
- Anti-Bacterial Agents, Ascomycota, Chromatography, High Pressure Liquid, Ferric Compounds, Hydroxamic Acids, Mixed Function Oxygenases, Hydroxylation, Iron, Ornithine, Saccharomyces cerevisiae, Mass Spectrometry, Vibrio, Siderophores
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