Characterization of microbial siderophores by mass spectrometry
Abstract
Siderophores play important roles in microbial iron piracy, and are applied as infectious disease biomarkers and novel pharmaceutical drugs. Inductively coupled plasma and molecular mass spectrometry (ICP‐MS) combined with high resolution separations allow characterization of siderophores in complex samples taking advantages of mass defect data filtering, tandem mass spectrometry, and iron‐containing compound quantitation. The enrichment approaches used in siderophore analysis and current ICP‐MS technologies are reviewed. The recent tools for fast dereplication of secondary metabolites and their databases are reported. This review on siderophores is concluded with their recent medical, biochemical, geochemical, and agricultural applications in mass spectrometry context. © 2015 Wiley Periodicals, Inc. Mass Spec Rev 35: 35–47, 2016.
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Previous techniques for elucidating metal-binding relied on properties of the metal-adduct itself, including metal-specific isotopic signatures, mass defect/Kendrick analysis, or hyperfine splitting isotope patterns.
“Previous techniques for elucidating metal-binding have relied on properties of the metal-adduct itself, including the presence of metal-specific isotopic signatures,87,88 mass defect/Kendrick analysis,89 or analysis of hyperfine splitting isotope patterns.90”
From Connecting metabolome and phenotype: recent advances in functional metabolomics tools for the identification of bioactive natural products (2024) · cites this paper as [89]
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- Authors
- Pluháček, T., Lemr, K., Ghosh, D., Milde, D., Novák, J., & Havlíček, V.
- Affiliations
- Regional Centre of Advanced Technologies and Materials (CZ)
- Jawaharlal Nehru University (IN)
- Czech Academy of Sciences, Institute of Microbiology (CZ)
- Department of Analytical Chemistry, Faculty of Science Regional Centre of Advanced Technologies and Materials Palacky University 17. listopadu 12 771 46 Olomouc Czech Republic
- Venue
- Mass Spectrometry Reviews
- Publisher
- Wiley
- Published
- 2015-05-15
- Volume
- 35
- Issue
- 1
- Pages
- 35-47
- ISSN
- 0277-7037 (ISSN-L)
- 1098-2787
- OpenAlex
- W1517104873
- Cited by
- 74
- References
- 105
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 6 · 2023: 9 · 2022: 5 · 2021: 6 · 2020: 12 · 2019: 3 · 2018: 10 · 2017: 5 · 2016: 9 · 2015: 2
- FWCI
- 0.83
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Mass Spectrometry Reviews (publishedVersion)
- PubMed (publishedVersion)
- ASEP (submittedVersion, public-domain)
- Funders
- Czech Science Foundation (P206/12/1150)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
- Keywords
- siderophores, inductively coupled plasma mass spectrometry, tandem mass spectrometry, iron compounds
- MeSH terms
- Biodegradation, Environmental, Chelating Agents, Drug Resistance, Microbial, Microbial Sensitivity Tests, Spectrophotometry, Atomic, Mass Spectrometry, Siderophores, Secondary Metabolism
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